Soil fertility influences leave quality of Morus alba L. in Mizoram, Northeast India

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Singh, Ngangbam Somen, Vanlalruati, Melody C., Tripathi, Shri Kant

Research Articles | Published: 18 February, 2022

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-022-00351-0
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Last Page: 0
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Keywords: Soil nutrients, Leaf quality, n Morus alba L. plantations, Proximate analysis, Total nitrogen


The present study was designed to assess the effect of soil fertility levels on leaf quality of Morus alba L. plantations (denoted by S1, S2, and S3) growing on different soil nutrient status in Mizoram. Soil samples (0–15 cm depth) were collected from 5 random locations from each site and analyzed for physico-chemical parameters. Further, mature M. alba leaves were randomly collected from different locations at the site and proximity analysis was conducted. The soil properties (i.e. soil total carbon, SC; total nitrogen, TN; available phosphate, Pavail; exchangeable K; Kexch, and microbial biomass carbon, MBC) in these sites were in the order: S3 > S2 > S1. All soil properties were significantly different in these sites except TN, Pavail and Kexch values, but they did not differ significantly in sites S2 and S3. Proximity analysis showed high moisture and protein contents (i.e. 69.02% and 157.8 mg g−1) and (i.e. 68.89% and 148.4 mg g−1) in S2 and S3, respectively, compared to S1 (68.3% and − 126.4 mg g−1). However, crude fiber, ether extract, total ash and acid insoluble ash contents were significantly higher in S1 compared to S2 and S3. Soil chemical properties were significantly (p < 0.01) positively correlated with crude protein and leaf moisture contents, whereas, significant (p < 0.05 and 0.01) negative correlations were observed between soil chemical properties and remaining leaf proximity parameters. It is concluded that soil nutrient content significantly affects the quality of M. alba leaves. Therefore, proper soil fertility management through periodical assessment is recommended for the production of quality leaves of M. alba to have better silk production in the region.

Soil nutrients, Leaf quality, n                     Morus alba L. plantations, Proximate analysis, Total nitrogen


Alipanah M, Abedian Z, Nasiri A, Sarjamei F (2020) Nutritional effects of three mulberry varieties on silkworms in Torbat Heydarieh. Psyche.

Allen SE, Grimshaw HM, Parkinson JA, Quarmby C (1974) Chemical analysis of ecological materials. Blackwell Scientific Publications.

Anderson JM, Ingram ISI (1993) Tropical soil biology and fertility—a handbook of methods, 2nd edn. CAB International, Wallingford.

AOAC (2005) Official methods of analysis, 18th edn. Association of Official Analytical Chemists, Arlington.

Bongale UD, Siddalingaswamy N (1996) Physico-chemical properties of soils from major sericultural areas of Kolar and Bangalore districts, Karnataka, India. Indian J Seric 35:152–154.

Brady NC (1984) The nature and properties of soil, 9th edn. Macmillan Publ. Co., New York, p 750.

Bray RH, Kurtz LT (1945) Determination of total, organic, and available forms of phosphorus in soils. Soil Sci 59:39–46.,_Organic,_and_Available.6.aspx

Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Bio Biochem 17:837–842.

Gee GW, Bauder JW (1986) Particle-size analysis 1. Methods of soil analysis: part 1—physical and mineralogical methods, (methodsofsoilan1), pp 383–411.

Ghosh S, Tripathi SK (2021) Microbial Succession and changes in carbon and nitrogen during decomposition of leaf litters of Tephrosia candida (Roxb.) DC. and Oryza sativa L. under shifting cultivation in Mizoram, Northeast India. J Appl Nat Sci 13:1032–1040.

Hauchhum R, Tripathi SK (2017) Rhizosphere effects of Melocanna baccifera on soil microbial properties under different fallow phases following shifting cultivation. Int J Plant Soil Sci 17:1–9.

Hauchhum R, Tripathi SK (2019) Carbon and nitrogen differences in rhizosphere soil of annual plants in abandoned lands following shifting agriculture in northeast India. Nutr Cycl Agroecosyst 113:157–166.

Hiratsuka E (1917) Researches on the nutrition of the silkworm. Shangai Shkenjo Hoko Ku. Tech Bull 2:353–412.

Hiware CJ (2006) Effect of fortification of Mulberry leaves with homeopathic drug Nux Vomica on Bombyx Mori L. Homeopathy 95:148–150.

Ito T, Kobayashi M (1978) Rearing of silkworm. In: Tazima Y (ed) The silkworm, an important laboratory tool. Kodansha Ltd, Tokyo, pp 83–102.

Krishnakumar AK, Datta AK, Potty SN (1990) Moisture retention characteristics of soils under Hevea in India. Indian J Rubber Res 3:9–21.

Lalnunzira C, Tripathi SK (2018) Leaf and root production, decomposition and carbon and nitrogen fluxes during stand development in tropical moist forests, north-east India. Soil Res 56:306–317.

Mallette MF, Althous PM, Clagetta CO (1960) Biochemistry of plants and animals.

Mary LCL, Sujatha R, Chozhaa AJ, Navas PMA (2015) Influence of organic manures (Biofertilizers) on soil microbial population in the rhizosphere of mulberry (Morus Indica L.). Int J Appl Sci Biotech 3:61–66.

Murugan K, Jeyabalan D, Senthil Kumar N, Senthil Nathan S, Sivaprakasam N (1998) Growth promoting effects of plant products on silkworm: a biotechnological approach. J Sci Indus Res 57:740–745.

Muthoni J, Kabira JN (2011) Effects of different sources of nitrogen on potato at Tigoni, Kenya. J Soil Sci Environ Manag 2:167–174.

Parpiev BA (1968) Water metabolism in silkworm fed with a different mulberry strain changing diet. Sheik 39:15–17.

Paul DC, Subba Rao G, Deb DC (1992) Impact of dietary moisture on nutritional indices and growth of Bombyx mori and concomitant larval duration. J Insect Physiol 38:229.

Pearson D (1976) The chemical analysis of foods (No. Ed. 7). Longman Group Ltd.

Prithivirajan R, Balasundar P, Shyamkumar R, Al-Harbi NS, Kadaikunnan S, Ramkumar T, Narayanasamy P (2019) Characterization of cellulosic fibers from Morus alba L. stem. J Nat Fibers 16:503–511.

Rangaswami G, Narasimhanna MN, Kasiviswanathan K, Sastry CR, Jolly MS (1976) Manual on sericulture. V. 1: mulberry cultivation. Agricultural Services Bulletin (FAO).

Ravikumar C (1988) Western ghat as a bivoltine region prospects, challenges and strategies for its development. Indian Silk 26:39–54.

Riaz M, Bukhari R, Sudan K (2020) Growth rate pattern and economic traits of silkworm, (Bombyx mori L) under the influence of inorganic and organic supplementation diet. Int J Fauna Biol Stud 8:5–7.

Samanta A, Chatterjee AK, Kar R, Mandal B (2001) Assessment of manganese content in mulberry garden soils of West Bengal. Ind J Seric 40:64–70.

Sarmah MC, Neog K, Das A, Phukan JCD (2013) Impact of soil fertility and leaf nutrients status on cocoon production of muga silkworm Antheraea assamensis (Helfer) in potential muga growing areas of Assam, India. Int J Curr Microbiol Appl Sci 2:25–38.

Seidavi AR, Bizhannia AR, Sourati R, Mavvajpour M (2005) The nutritional effects of different mulberry varieties on biological characters in silkworm. Asia Pac J Clin Nutr 14:S122–S122.

Shilpashree KG, Subbarayappa CT, Doreswamy S (2015) Effect of soil application of micronutrients on quality of mulberry and cocoon production. Res J Agric Sci 6:830–833.

Shrestha S, Kazama F (2007) Assessment of surface water quality using multivariate statistical techniques: a case study of the Fuji river basin, Japan. Environ Mod Softw 22:464–475.

Singh NS, Tripathi SK (2020) Litter mass loss rate changes as function of soil microbial diversity and litter chemical quality in tropical and sub-tropical forest of Mizoram: a microcosm study. Indian J Ecol 47:792–798.

Singh SB, Mishra BP, Tripathi SK (2015) Recovery of plant diversity and soil nutrients during stand development in subtropical forests of Mizoram. Northeast India. Biodiversitas 16:205–212.

Sudhakara SN, Narayanaswamy TK, Narayanaswamy KC (2017) Role of integrated use of organic manures and inorganic fertilizers on mulberry and its impact on cocoon parameters of silkworm (Bombyx mori L.). Int J Curr Microbiol Appl Sci 6:3922–3927.

Thangavelu K, Sahu AK (1986) Further studies on the in-door rearing of muga silkworm, Antheraea assama Westw.(Saturniidae: Lepidoptera). Sericologia 26:215–227.

Thimmareddy H, Prabhuraj DK, Bongale UD, Dandin SB (1999) Fertility status of mulberry growing soils in Mysore seed area, Karnataka. Indian J Seric 38: 26–29.

Tripathi SK, Vanlalfakawma DC, Lalnunmawia F (2017) Shifting cultivation on steep slopes of Mizoram, India: impact of policy reforms. In: Malcom C (ed) Shifting cultivation policies: balancing environmental and social sustainability. CABI International, pp 393–413.



Authors are thankful to the Sericulture Department, Government of Mizoram, the Department of Animal Nutrition, the College of Veterinary Science and Animal Husbandry, Central Agriculture University, Mizoram and the Department of Forestry, Mizoram University for providing laboratory, resources, M. alba leaves and other assistance during course of study.

Author Information

Singh, Ngangbam Somen
Department of Forestry, School of Earth Science and Natural Resources Management, Mizoram University, Aizawl, India

Vanlalruati, Melody C.
Department of Forestry, School of Earth Science and Natural Resources Management, Mizoram University, Aizawl, India

Tripathi, Shri Kant
Department of Forestry, School of Earth Science and Natural Resources Management, Mizoram University, Aizawl, India