Antioxidant and antibacterial activities evaluation of the hydroethanolic extract of Cyperus rotundus L. and its fractions

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Siroua, Karima, Belamine, Habiba, Kadiri, Fatimazahra, Ait Haj Said, Amal, Blaghen, Mohammed, El Kouali, Mhamed, Kenz, Abdelkbir


Research Articles | Published: 10 March, 2022

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00356-9
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Keywords: n Cyperus rotundusn , Rhizome, Antioxidant activity, Total phenolic content, Free radical scavenging, Antibacterial activity


Abstract


Cyperus rotundus is a medicinal plant used in various countries, despite being very scarcely studied in Morocco, it is still exploited in the traditional Moroccan medicine. In this present work, a cold maceration of the Cyperus rotundus rhizomes was carried out, followed by a Liquid–Liquid Extraction of the obtained crude extract using different solvents with ascending polarity. Then, polyphenol and flavonoid contents were measured in the crude extract and its fractions. Antioxidant activity was also studied through free radical scavenging tests (ABTS and DPPH), and antibacterial activity was investigated by the agar diffusion assay method on four bacterial strains: two Gram-positive and two Gram-negative. The latter activities were performed on Cyperus rotundus rhizomes crude extract as well as its different fractions.

n              Cyperus rotundusn            , Rhizome, Antioxidant activity, Total phenolic content, Free radical scavenging, Antibacterial activity


References


Ahn MR, Kumazawa S, Usui Y et al (2007) Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem 101:1383–1392. https://doi.org/10.1016/j.foodchem.2006.03.045


Al Jahid A, Essabaq S, Elamrani A et al (2016) Chemical composition, antimicrobial and antioxidant activities of the essential oil and the hydro-alcoholic extract of Artemisia campestris L. leaves from Southeastern Morocco. J Biol Act Prod from Nat 6:393–405. https://doi.org/10.1080/22311866.2016.1268068


Albano SM, Miguel MG (2011) Biological activities of extracts of plants grown in Portugal. Ind Crops Prod 33:338–343. https://doi.org/10.1016/j.indcrop.2010.11.012


Almahi IM, Beseni BK, Msomi NZ et al (2021) The antioxidant and antidiabetic potentials of polyphenolic-rich extracts of Cyperus rotundus (Linn.). J Biomol Struct Dyn. https://doi.org/10.1080/07391102.2021.1967197


Appelbaum PC (2012) 2012 and beyond: potential for the start of a second pre-antibiotic era? J Antimicrob Chemother 67:2062–2068. https://doi.org/10.1093/jac/dks213


Bajpai M, Pande A, Tewari SK, Prakash D (2005) Phenolic contents and antioxidant activity of some food and medicinal plants. Int J Food Sci Nutr 56:287–291. https://doi.org/10.1080/09637480500146606


Berrichi A, Bouhache M, RedaTazi M (2003) État d’infestation de la plaine des Triffa (Maroc oriental) par le souchet à tubercules (Cyperus rotundus L.). Actes L’institut Agron Vétérinaire Hassan II 23:33–69


Bouchouka E (2016) Extraction des polyphénols et étude des activités antioxydante et antibactérienne de quelques plantes Sahariennes. Badji Mokhtar Annaba University—Faculty of Sciences


Bourkhiss M, Hnach M, Paolini J et al (2010) Propriétés antioxydantes et anti-inflammatoires des huiles essentielles des différentes parties de Tetraclinis Articulata. Bull La Soc R Des Sci Liège 79:141–154


Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30. https://doi.org/10.1016/S0023-6438(95)80008-5


Corrales Ramírez L, Castillo Castañeda A, Melo Vargas A (2013) Evaluación del potencial antibacterial in vitro de Croton lechleri frente a aislamientos bacterianos de pacientes con úlceras cutáneas. Nova 11:51–63. https://doi.org/10.22490/24629448.1018


del Vega-Menchaca MC, Verde-Star J, Oranday-Cárdenas A et al (2013) Actividad antibacteriana y citotóxica de Leucophyllum frutescens (Berl) I.M. Johnst del Norte de México contra Staphylococcus aureus de aislados clínicos. Rev Mex Ciencias Farm 44:24–30


Dhar P, Dhar DG, Rawat AKS, Srivastava S (2017) Medicinal chemistry and biological potential of Cyperus rotundus Linn.: an overview to discover elite chemotype(s) for industrial use. Ind Crops Prod 108:232–247. https://doi.org/10.1016/j.indcrop.2017.05.053


Dif MM, Benyahia M, ToumiBenali F et al (2017) Phenolic content and antioxidant activity of three algerian species of lavenders. Phytotherapie 15:367–372. https://doi.org/10.1007/s10298-016-1028-5


Dorman HJD, Hiltunen R (2004) Fe(III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food Chem 88:193–199. https://doi.org/10.1016/j.foodchem.2003.12.039


Fair RJ, Tor Y (2014) Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem. https://doi.org/10.4137/PMC.S14459


Ghasemi K, Ghasemi Y, Ebrahimzadeh MA (2009) Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak J Pharm Sci 22:277–281


Gibbons S (2005) Plants as a source of bacterial resistance modulators and anti-infective agents. Phytochem Rev 4:63–78. https://doi.org/10.1007/s11101-005-2494-9


Guo L, Xie MY, Yan AP et al (2006) Simultaneous determination of five synthetic antioxidants in edible vegetable oil by GC-MS. Anal Bioanal Chem 386:1881–1887. https://doi.org/10.1007/s00216-006-0738-1


Hagerman AE, Riedl KM, Jones GA et al (1998) High molecular weight plant polyphenolics (Tannins) as biological antioxidants. J Agric Food Chem 46:1887–1892. https://doi.org/10.1021/jf970975b


Hebi M, Eddouks M (2016) Évaluation de l’activité antioxydante de Stevia rebaudiana. Phytotherapie 14:17–22. https://doi.org/10.1007/s10298-015-0999-y


Hennebelle T, Sahpaz S, Bailleul F (2004) Polyphénols végétaux, sources, utilisations et potentiel dans la lutte contre le stress oxydatif. Phytothérapie 2:3–6. https://doi.org/10.1007/s10298-004-0003-8


Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841–1856. https://doi.org/10.1021/jf030723c


Iserin P, Masson M, Restellini J-P (2001) Encyclopédie des plantes médicinales - Identification, Préparations, Soins, Larousse


Kabbashi AS, Mohammed SEA, Almagboul AZ, Ahmed IF (2015) Antimicrobial activity and cytotoxicity of ethanolic extract of Cyperus rotundus L. Am J Pharm Pharm Sci 2:1–13


Kilani S, Ben Sghaier M, Limem I et al (2008) In vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of Cyperus rotundus. Bioresour Technol 99:9004–9008. https://doi.org/10.1016/j.biortech.2008.04.066


Kilani-Jaziri S, Bhouri W, Skandrani I et al (2011) Phytochemical, antimicrobial, antioxidant and antigenotoxic potentials of Cyperus rotundus extracts, South African. J Bot 77:767–776. https://doi.org/10.1016/j.sajb.2011.03.015


Köser CU, Ellington MJ, Cartwright EJP et al (2012) Routine use of microbial whole genome sequencing in diagnostic and public health microbiology. PLoS Pathog 8:1–9. https://doi.org/10.1371/journal.ppat.1002824


Kumar GS, Nayaka H, Dharmesh SM, Salimath PV (2006) Free and bound phenolic antioxidants in amla (Emblica officinalis) and turmeric (Curcuma longa). J Food Compos Anal 19:446–452. https://doi.org/10.1016/j.jfca.2005.12.015


Mandal N, Mandal S, Hazra B et al (2011) Assessment of the antioxidant and reactive oxygen species scavenging activity of methanolic extract of Caesalpinia crista Leaf. Evid Based Compl Altern Med. https://doi.org/10.1093/ecam/nep072


Medini F, Ben Hamida M, Atwi A, Ksouri R (2018) Effect of eco-extraction on polyphenols contents and antioxidant activity of Lepidium sativum seeds. J New Sci Agric Biotechnol 54:3594–3602


Mensor LL, Menezes FS, Leitao GG et al (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phyther Res 15:127–130. https://doi.org/10.1002/ptr.687


Nagulendran KR, Velavan S, Mahesh R, Hazeena Begum V (2007) In vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. E J Chem 4:440–449. https://doi.org/10.1155/2007/903496


Nickavar B, Kamalinejad M, Izadpanah H (2007) In vitro free radical scavenging activity of five Salvia species. Pak J Pharm Sci 20:291–294


Odabasoglu F, Aslan A, Cakir A et al (2004) Comparison of antioxidant activity and phenolic content of three lichen species. Phyther Res 18:938–941. https://doi.org/10.1002/ptr.1488


Pousset J-L (1989) Plantes médicinales africaines—Utilisation Pratique, ACCT. Ellipses, Paris


Sengul M, Yildiz H, Gungor N et al (2009) Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 22:102–106


Shah BN, Seth AK (2009) Textbook of pharmacognosy and phytochemistry. Elsevier India


Singleton VL, Joseph A, Rossi J (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158


Trabsa H (2015) Activité antioxydante et anti-inflammatoire des fractions des plantes médicinales: Sedum sediforme et Lycium arabicum. Ferhat Abbas Setif 1 University—Faculty of Sciences of Nature and Life


Valko M, Rhodes CJ, Moncol J et al (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40. https://doi.org/10.1016/j.cbi.2005.12.009


Vallejo A, Feitosa A, Gourlart AE et al (2014) Tamizaje de acción antimicrobiana de 34 extractos vegetales contra bacilos gramnegativos. Salud Soc 1:34–39. https://doi.org/10.19053/19092407.3257


Wong CC, Bin LH, Cheng KW, Chen F (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 97:705–711. https://doi.org/10.1016/j.foodchem.2005.05.049

 


Acknowledgements


We thank all the authors who contributed to this work.


Author Information


Siroua, Karima
Laboratoire de Chimie Analytique et Physico Chimie des Matériaux, Faculté des Sciences Ben M’sik, Hassan II University of Casablanca, Casablanca, Morocco
karima.siroua@gmail.com
Belamine, Habiba
Laboratoire Sciences des Médicaments, Recherche Biomédicale et Biotechnologie, Faculté de Médecine et de Pharmacie, Hassan II University of Casablanca, Casablanca, Morocco


Kadiri, Fatimazahra
Laboratoire de Microbiologie, Pharmacologie, Biotechnologie et Environnement, Faculté des Sciences Aïn Chock, Hassan II University of Casablanca, Casablanca, Morocco

Ait Haj Said, Amal
Laboratoire Sciences des Médicaments, Recherche Biomédicale et Biotechnologie, Faculté de Médecine et de Pharmacie, Hassan II University of Casablanca, Casablanca, Morocco

Blaghen, Mohammed
Laboratoire de Microbiologie, Pharmacologie, Biotechnologie et Environnement, Faculté des Sciences Aïn Chock, Hassan II University of Casablanca, Casablanca, Morocco

El Kouali, Mhamed
Laboratoire de Chimie Analytique et Physico Chimie des Matériaux, Faculté des Sciences Ben M’sik, Hassan II University of Casablanca, Casablanca, Morocco

Kenz, Abdelkbir
Laboratoire de Chimie Analytique et Physico Chimie des Matériaux, Faculté des Sciences Ben M’sik, Hassan II University of Casablanca, Casablanca, Morocco