Deciphering antimicrobial, phytochemical, GC–MS and pharmacokinetic properties of Camellia sinensis from high-altitude region

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00368-5
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Keywords: Camellia sinensis , Antimicrobial, Phytochemical, Gas chromatography–mass spectrometry, SwissADME


Abstract


Camellia sinensis of high altitude region possesses wide-range of antimicrobial activity due to the presence of various bioactive components. The qualitative screening of C. sinensis was carried out to detect the presence of various phytocompounds viz., carbohydrates, alkaloids, tannins, flavonoids, saponins, terpenoids, amino acids and steroids. The antimicrobial activity was evaluated by agar-well diffusion method and based on the presence of zone of inhibition around the wells, the most potent extract was used for further studies. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of C. sinensis were also determined against all the selected pathogenic microorganisms. Among all extracts, acetone extract of C. sinensis showed the most potent inhibitory activity and therefore, was used for further activities.The lowest MIC (0.23 mg/mL) and MBC (0.48 mg/mL) was observed against Staphylococcus aureus. A total of eight bioactive compounds were determined by GC–MS analysis. Out of eight compounds, Decane, 1-bromo 2-methyl was identified as the major compound having the highest peak area of 24.21%. This compound was subjected to SwissADME to determine the pharmaceutical potential for new drug discovery.



              Camellia sinensis
            , Antimicrobial, Phytochemical, Gas chromatography–mass spectrometry, SwissADME


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Acknowledgements


The authors acknowledge the Head of the Department of Botany and Microbiology for providing laboratory facilities.


Author Information


Pradhan Surbhi
Department of Botany and Microbiology, Gurukula Kangri (Deemed To Be University), Haridwar, India
surbhipradhan93@gmail.com