In vitro shoot formation and enrooted mini-corm production by direct organogenesis in saffron (crocus sativus L.)

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-023-00639-9
First Page: 1045
Last Page: 1050
Views: 119

Keywords: Saffron, Regeneration, Bud sprouting, Adventitious shoots, Progeny corms


Saffron’s triploidy and male sterility result in generative limitations that highly impact its regeneration. Therefore, its natural reproduction is exclusively vegetative through progeny corms. The present work aims to improve in vitro saffron bud sprouting and adventitious shoot regeneration through direct organogenesis using different combinations of plant growth regulators, and then investigate novel conditions for mini-corm production. Murashige and Skoog medium (MS) containing 1 mg/L of 6-benzyl aminopurine (BAP) and α-naphthalene acetic acid (NAA) resulted in the highest bud sprouting rate (96.67%) and growth length (8.87 cm ± 0.27 cm). The best adventitious shoot initiation rate of 80% with 10.2 ± 0.23 shoots per explant was obtained using 0.5 mg/L NAA and 2.75 mg/L BAP. Remarkably, the critical step of mini-corm regeneration was improved using ½ MS, 6% sucrose, 1 mg/L NAA, and dark incubation. The corms produced weighed 7.9 ± 0.8 g, and 93.80% developed roots with an average of 14.9 ± 3.1 roots per corm. Improving the critical stages of saffron tissue culture is essential to meet the urgent need for its large-scale and low-cost regeneration.

Saffron, Regeneration, Bud sprouting, Adventitious shoots, Progeny corms

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The authors appreciate Souktana Cooperative and Dar Azaafarane’s supply of plant materials.

Author Information

Lagram Khalid
Department of Environment and Life Sciences, Faculty of Applied Sciences, Ibn Zohr University, Agadir, Morocco