A validated HPTLC method for quantification of active compounds in a Polyherbal formulation Guggulutiktam Kashayam

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Research Articles | Published:

E-ISSN: 2229-4473.
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DOI: 10.1007/s42535-025-01195-0
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Keywords: Guggulutiktam Kashayam, HPTLC, Polyherbal formulation, Caffeic acid, Scopoletin


Abstract

Guggulutiktam Kashayam (GTK) is an important Polyherbal formulation used in Ayurveda for the treatment of various ailments such as ulcers, cardiac diseases, skin disorders, arthritis and cancer. It is prepared from parts of 29 plants. Polyherbal formulations are rich in active phytochemicals. Active phytochemicals like Caffeic acid and Scopoletin have been quantified in GTK by a validated HPTLC method. Analysis was carried out using CAMAG HPTLC system equipped with auto sampler, documentation unit and densitometry scanner. The method was validated in terms of assessing precision, accuracy, linearity, specificity, robustness, limit of detection (LOD) and limit of quantification (LOQ) as per the International Council for Harmonization (ICH) Guidelines. The quantity of Caffeic acid and Scopoletin in GTK are found to be 0.0262 (%w/w) and 0.00209 (%w/w) respectively. A novel and validated HPTLC method has been developed for the quantification of active compounds of an important Ayurvedic formulation which can be used as a powerful analytical tool for the quality standardization of the formulation. This method can also be used for the quality checking of similar types of other Polyherbal formulations.

Guggulutiktam Kashayam, HPTLC, Polyherbal formulation, Caffeic acid, Scopoletin


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Author Information

Phytochemistry Division, Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, India