Altitudinal variation in gallic acid content in fruits of Phyllanthus emblica L. and its correlation with antioxidant and antimicrobial activity

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00048-x
First Page: 387
Last Page: 396
Views: 1873


Keywords: Antibacterial, Antioxidant, HPTLC, Phyllamthus emblica , Phytochemical variation


Abstract


Gallic acid is one of the most important organic compounds in fruits of Phyllanthus emblica. Therefore, the objective of the present study is to find out the effect of altitude on gallic acid content in fruits of P. emblica and its correlation with antioxidant and antimicrobial activity. Phytochemicals such as phenolics, tannins, flavonoids, carbohydrates, glycosides, phytosteroids, alkaloids and saponins were detected in fruit extracts of P. emblica collected from different regions of Himachal Pradesh. Fruits extract from Mandi (239.74 ± 39.28 mg/g gallic acid equivalents, GAE) district showed a higher amount of total phenolic content (TPC), whereas, total flavonoid content (TFC) was higher from Kangra (356 ± 27.63 mg/g rutin equivalents, RE) district. Methanolic extracts showed inhibition to the growth of both Gram-positive (B. subtilis, S. aureus), and Gram-negative bacteria (E. coli, K. pneumoniae). The methanolic extract of fruits of Bilaspur district showed the highest antibacterial activity against B. subtilis (19.5 ± 0.71 mm), S. aureus (21.0 ± 1.41 mm), E. coli (17.5 ± 0.71) and K. pneumoniae (21.5 ± 2.12) as compared to other regions and amoxyclav. High-performance thin-layer chromatography (HPTLC) method was used for the quantification of gallic acid in the extracts of fruits of P. emblica. HPTLC chromatogram showed the highest content of gallic acid in methanolic extracts of fruits from Kangra followed by Bilaspur, Mandi, and Una. However, antioxidant and antibacterial activity was higher in fruits extracts of high altitude (Bilaspur region). In summary, Bilaspur region of Himachal Pradesh could be used for mass cultivation of fruits of P. emblica because of their high antioxidant and antimicrobial potential under these geographical conditions.


Antibacterial, Antioxidant, HPTLC, 
                Phyllamthus emblica
              , Phytochemical variation


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References


  1. Abbasi S, Daneshfar A, Hamdghadareh S, Farmany A (2011) Quantification of sub-nanomolar levels of gallic acid by adsorptive stripping voltammetry. Int J Electrochem Sci 6:4843–4852

  2. Al-Gbouri NM, Hamzah AM (2018) Evaluation of Phyllanthus emblica extracts as antibacterial and antibiofilm against biofilm formation bacteria. Iraqi J Agric Sci 49:142–151

  3. Alonso C, Pérez R, Nieto PM, Delgado J (2005) Gender dimorphism and altitudinal variation of secondary compounds in leaves of the gynodioecious shrub Daphne laureola. J Chem Ecol 31:139–150

  4. Anila L, Vijayalakshmi NR (2002) Flavonoids from Emblica officinalis and Mangifera indica—effectiveness for dyslipidemia. J Ethnopharmacol 79:81–87

  5. Bajpai M, Pande A, Tewari SK, Prakash D (2005) Phenolic contents and antioxidant activity of some food and medicinal plants. Int J Food Sci Nutr 56:287–291

  6. Barros L, Ferreira MJ, Queiros B, Ferreira ICFR, Baptista P (2007) Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 103:413–419

  7. Beniwal V, Kumar A, Sharma J, Chhokar V (2013) Recent advances in industrial application of tannases: a review. Recent Pat Biotechnol 7:228–233

  8. Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay. Anal Biochem 239:70–76

  9. Bhattacharya SK, Bhattacharya A, Sairam K, Ghosal S (2002) Effect of bioactive tannoid principles of Emblica officinalis on ischemia-reperfusion-induced oxidative stress in rat heart. Phytomedicine 9:171–174

  10. Carey DB, Wink M (1994) Elevational variation of quinolizidine alkaloid contents in a lupine (Lupinus argenteus) of the Rocky Mountains. J Chem Ecol 20:849–857

  11. Chandel SR, Kumar V, Guleria S, Sharma N, Sourirajan A, Khosla PK, Baumler DJ, Dev K (2019) Sequential fractionation by organic solvents enhances the antioxidant and antibacterial activity of ethanolic extracts of fruits and leaves of Terminalia bellerica from North Western Himalayas, India. Pharmacogn J 11:94–101

  12. Charmkar NK, Singh R (2017) Emblica officinalis Gaertn (Amla): a wonder gift of nature to humans. Int J Curr Microbiol App Sci 6:4267–4280

  13. Chaudhary HJ, Wadhwa BM (1984) Flora of Himachal Pradesh, vol 1–3. Botanical Survey of India, Howrah

  14. Choubey S, Varughese LR, Kumar V, Beniwal V (2015) Medicinal importance of gallic acid and its ester derivatives: a patent review. Pharm Pat Anal 4:305–315

  15. Choudhry N, Singh S, Siddiqui MB, Khatoon S (2014) Impact of seasons and dioecy on therapeutic phytoconstituents of Tinospora cordifolia, a Rasayana drug. Biomed Res Int 2014:11

  16. Dey A, Pandey DK (2014) HPTLC detection of altitudinal variation of the potential antivenin stigmasterol in different populations of the tropical ethnic antidote Rauvolfia serpentina. Asian Pac J Trop Med 7:540–545

  17. Fidrianny I, Suhendy H, Insanu M (2018) Correlation of phytochemical content with antioxidant potential of various sweet potato (Ipomoea batatas) in West Java, Indonesia. Asian Pac J Trop Biomed 8:25–30

  18. Gaire BP, Subedi L (2014) Phytochemistry, pharmacology and medicinal properties of Phyllanthus emblica Linn. Chin J Integr Med 9:1–8

  19. Gairola S, Shariff NM, Bhatt A, Kala CP (2010) Influence of climate change on production of secondary chemicals in high altitude medicinal plants: issues needs immediate attention. J Med Plants Res 4:1825–1829

  20. Gali HU, Perchellet EM, Perchellet JP (1991) Inhibition of tumor promoter-induced ornithine decarboxylase activity by tannic acid and other polyphenols in mouse epidermis in vivo. Cancer Res 51:2820–2825

  21. Gan J, Feng Y, He Z, Li X, Zhang H (2017) Correlations between antioxidant activity and alkaloids and phenols of Maca (Lepidium meyenii). J Food Quality. https://doi.org/10.1155/2017/3185945

  22. Gangwar KK, Deepali Gangwar RS (2010) Ethnomedicinal plant diversity in Kumaun Himalaya of Uttarakhand India. Nat Sci 8:66–78

  23. Harbone JB (1998) Phytochemical Methods, 3rd edn. Chapman and Hall, London, pp 117–119

  24. Hazra B, Sarkar R, Biswas S, Mandal N (2010) Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis. BMC Complement Altern Med 10:20. https://doi.org/10.1186/1472-6882-10-20

  25. Jamil K (2017) Estimation of Antibacterial Activity of Plants Extracts from Phyllanthus emblica, Terminalia Chebula and Eucalyptus globulus against oral pathogens. Int J Dent Oral Heal 3:9–100

  26. Khoo HE, Azlan A, Kong KW, Ismail A (2016) Phytochemicals and medicinal properties of indigenous tropical fruits with potential for commercial development. Evid Based Complement Altern Med 2016:2016. https://doi.org/10.1155/2016/7591951

  27. Kumar V, Sharma N, Sourirajan A, Khosla PK, Dev K (2018) Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India. J Tradit Complement Med 8:100–106

  28. Kumaran A, Karunakaran RJ (2006) Nitric oxide radical scavenging active components from Phyllanthus emblica L. Plant Foods Hum Nutr 61(1):1–5

  29. Mirunalini S, Krishnaveni M (2010) Therapeutic potential of Phyllanthus emblica (amla): the ayurvedic wonder. J Basic Clin Physiol Pharmacol 21:93–105

  30. Naik GH, Priyadarsini KI, Bhagirathi RG et al (2005a) In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents. Phytother Res 19:582–586

  31. Naik GH, Priyadarsini KI, Mohan H (2005b) Evaluating the antioxidant activity of different plant extracts and herbal formulations. Res Chem Intermed 31:145–151

  32. Nickavar B, Adeli A, Nickavar A (2014) TLC-Bioautography and GC-MS analyses for detection and identification of antioxidant constituents of Trachyspermum copticum essential oil. Iran J Pharm Res 13:127–133

  33. Nisha P, Singhal RS, Pandit AB (2004) A study on degradation kinetics of ascorbic acid in amla (Phyllanthus emblica L.) during cooking. Int J Food Sci Nutr 55:415–422

  34. Pandey G, Khatoon S, Pandey MM, Rawat AK (2018) Altitudinal variation of berberine, total phenolics and flavonoid content in Thalictrum foliolosum and their correlation with antimicrobial and antioxidant activities. J Ayurveda Integr Med 9:169–176

  35. Perez C, Paul M, Bazerque P (1990) Antibiotic assay by agar-well diffusion method. Acta Biol Med Exp 15:113–115

  36. Petridis A, Therios I, Samouris G, Tananaki C (2012) Salinity-induced changes in phenolic compounds in leaves and roots of four olive cultivars (Olea europaea L.) and their relationship to antioxidant activity. Environ Exp Bot 79:37–43

  37. Poltanov EA, Shikov AN, Dorman HJ et al (2009) Chemical and antioxidant evaluation of Indian gooseberry (Emblica officinalis Gaertn., syn. Phyllanthus emblica L.) supplements. Phytother Res 23:1309–1315

  38. Rakholiya K, Vaghela P, Rathod T, Chanda S (2014) Comparative study of hydroalcoholic extracts of Momordica charantia L. against foodborne pathogens. Ind J Pharm Sci 76:148–156

  39. Ruiz-Terán F, Medrano-Martínez A, Navarro-Ocaña A (2008) Antioxidant and free radical scavenging activities of plant extracts used in traditional medicine in Mexico. Afr J Biotechnol 7:1886–1893

  40. Rumzhum NN, Rahman MM, Kazal MK (2012) Antioxidant and cytotoxic potential of methanol extract of Tabernaemontana divaricata leaves. Int Curr Pharm J 1:27–31

  41. Samal J (2016) Medicinal plants and related developments in India: a peep into 5-year plans of India. Indian J Health Sci Biomed Res (KLEU) 9:14

  42. Samant SS, Pant S, Singh M, Lal M, Singh A, Sharma A, Bhandari S (2007) Medicinal plants in Himachal Pradesh, north western Himalaya, India. Int J Biodivers Sci Manag 3:234–251

  43. Scartezzini P, Speroni E (2000) Review on some plants of Indian traditional medicine with antioxidant activity. J Ethnopharmacol 71:23–43

  44. Shan S, Huang X, Shah MH, Abbasi AM (2019) Evaluation of polyphenolics content and antioxidant activity in edible wild fruits. BioMed Res Int. https://doi.org/10.1155/2019/1381989

  45. Sharma RN, Bala J, Singh A, Prabhjot K (2011) Antibacterial potential of Achyranthus aspera Linn procured from Himachal Pradesh, Punjab and Haryana, India. Res J Chem Sci 8:80–82

  46. Shiva MP (1996) Inventory of forestry resources for sustainable management and biodiversity conservation. Indus Publishing Company, New Delhi

  47. Singleton VL (1981) Naturally occurring food toxicants: phenolic substances of plant origin common in foods. Adv Food Res 27:149–242

  48. Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, vol 299. Academic press, London, pp 152–178

  49. Vaquero MR, Alberto MR, De Nadra MM (2007) Antibacterial effect of phenolic compounds from different wines. Food Control 18:93–101

  50. Zhang YJ, Abe T, Tanaka T, Yang CR, Kouno I (2001) Phyllanemblinins A-F, new ellagitannins from Phyllanthus Emblica. J Nat Prod 64:1527–1532

  51. Zhang YJ, Nagao T, Tanaka T, Yang CR, Okabe H, Kouno I (2004) Antiproliferative activity of the main constituents from Phyllanthus emblica. Biol Pharm Bull 27:251–255

  52. Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

  53. Zidorn C (2010) Altitudinal variation of secondary metabolites in flowering heads of the Asteraceae: trends and causes. Phytochem Rev 9:197–203


  54.  


Acknowledgements


The authors acknowledge Shoolini University, Solan, for providing infrastructure support to conduct the research work. Authors also acknowledge the support provided by Yeast Biology Laboratory, School of Biotechnology and Central Instrumentation laboratory, School of Pharmaceutical Sciences, Shoolini University, Solan, Himachal Pradesh, India.


Author Information


Sheoran Shagun
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India

Nidhi Prakriti
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India


Kumar Vikas
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India


Singh Gajender
School of Pharmaceutical Sciences, Shoolini University of Biotechnology and Management Sciences, Bajhol, India


Lal Uma Ranjan
Department of Natural Products, National Institute of Pharmaceutical Education, Ahmedabad, India

Sourirajan Anuradha
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India
asourirajan@gmail.com
Dev Kamal
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India
kamaldevbhardwaj1969@gmail.com