Vegetos

Abstract

Okra [Abelmoschus esculenta (L.) Moench] of all the 30 genotypes were sown during the zaid season at Horticulture Research Centre, Department of Horticulture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, developed and maintained for using conventional agronomically practices to keep the crop in good condition. Study of molecular in landrace collections was carried out during 2013. All genotypes were screened with eight RAPD primers in order to determine genetic diversity and genetic relationships. A total of 27 bands were detected using 8 polymorphic RAPD primers out of which 18 bands were polymorphic and 9 were monomorphic bands thus generating 61.57% of polymorphism with an average of 3.375 bands per primer, Maximum numbers of bands was obtained by primer OPA-1 (seven alleles). PIC value ranged from 0.75 (OPA4) to 0.998 (OPA-2) with average of 0.913. Thus, this indicated that RAPD primer OPA-2 used in the study was most polymorphic, Resolving power (Rp) varied from a lower value of 0.066 (OPA-2) to a higher value of 1.773 (OPA-5) with average of 1.166. The gene diversity was varied from 1.00 to 0.43 values. Cluster analysis clearly showed the genetic diversity among the genotypes under study.