Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Analysis of microsatellites (SSRs) of Curvularia geniculata strain SKG23 isolated from leaf spot disease of Mentha (Mentha arvensis L.)
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Research Articles | Published: 30 June, 2026
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Keywords: Fungus, Genome, Sequence, Microsatellite
Abstract
Microsatellites, or SSRs (simple sequence repeats), are now used in several genome-related investigations, including DNA fingerprinting, linkage mapping, and population evolutionary studies. In an earlier publication (Ghosh and Polley 2025), we reported whole genome sequencing of the Curvularia geniculata strain SKG23, an important pathogen of the medicinal plant Mentha arvensis L. Here, we present the results of our study on the occurrence and composition of SSRs in this species. SSR analysis exhibited that the hexanucleotide repeat was the longest, bearing an average length of 31.24 bp, followed by penta- (22.81), di- (21.78), tri- (19.57), tetra- (19.00 bp), and mononucleotide repeat (15.48 bp), which was the shortest average length. The result also showed that the percentage of trinucleotide perfect SSRs was the highest, followed by di-, tetra-, mono-, and penta-, and the lowest percentage was in the case of hexanucleotide. Furthermore, the total relative abundance (RA) (loci/Mb) and total relative density (RD) (bp/Mb) of the perfect SSR of this fungus have been calculated, and we found the highest RA in trinucleotides (18.53), followed by di- (11.3), tetra- (6.89), mono- (5.48), and penta- (3.96), but the lowest was recorded in hexanucleotides (3.17). RD was highest in trinucleotide (362.53), followed by di- (245.98), tetra- (130.93), hexa- (99.06), and penta- (90.28), but the lowest was found in mononucleotide (84.81 bp/Mb). Therefore, the analysis of SSRs of this pathogenic fungus may serve as a marker for pathogenicity and environmental adaptability after experimental validation.
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Author Information
Molecular Mycopathology Lab, Biocontrol Unit and Cancer Research Unit, Ramakrishna Mission Vivekananda Centenary College (Autonomous), Rahara, India