Anti-fungal, anti-oxidant and phytochemical analysis of Plumbago zeylanica L.


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-020-00102-z
First Page: 247
Last Page: 257
Views: 516

Keywords: Medicinal properties, Inhibitory activities, Natural compounds, Free radicals, GC–MS analysis


Free radicals play a major role in development of many human diseases like cancer etc. In the present investigation Plumbago zeylanica (Pz) was used for anti-fungal activity, anti-oxidant activity as well as phytochemicals investigation. Anti-fungal activities were determined against four pathogenic fungal species Fusarium oxysporum, Rhizoctonia solanii, Alternaria sp. and Sclerotium rolfsii. Anti-oxidant activity and phytochemical investigation were determined by standard method using spectrophotometer, gas chromatography and mass spectroscopy. The excellent inhibitory activities were observed against Alternaria spp. (98%), F. oxysporum (68%), R. solanii (97.5%) followed by S. rolfsii (93.4%) at 62.5 μg/ml. The P. zeylanica showed free radicals scavenging activity by DPPH method (77.8% inhibition at 1000 µg/ml), CUPRAC assay (0.07940 TE mM at 1000 µg/ml), Anthocyanin content (2.404 CGE mg/ml), Flavonoid content (55,330 GAE/g), Phenols (14.75 µg/ml), Alkaloids (54 mg/g), Saponins (3.95%) and Tannin (7 µg/10 ml). So P. zeylanica extracts can be used as a source of bio-fungicide and scavenger of free radicals.

Medicinal properties, Inhibitory activities, Natural compounds, Free radicals, GC–MS analysis

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  1. Abidemi OO (2013) Phytochemicals and spectrophotometric determination of metals in various medicinal plants in Nigeria. Int J Eng Sci Invent 2(5):51–54

  2. Assefa AD, Keum YS (2017) Effect of extraction solvent and various drying methods on polyphenol content and antioxidant activities of yuzu (Citrus junos Sieb ex Tanaka). J Food Meas Charact 11(2):576–585

  3. Banu S, Arunachalam G, Jayaveera KN, Ashoka Babu VL, Premakumari KB (2011) Estimation of total phenolic content and in vitro anti-oxidant activity of Barleria montana. Der Pharm Lett 3(4):178

  4. Diplock AT, Charuleux JL, Crozier-Willi G, Kok FJ, Rice-Evans C, Roberfroid M, Vina-Ribes J (1998) Functional food science and defence against reactive oxidative species. Br J Nutr 80(1):77–112

  5. Giusti MM, Wrolstad RE (2001) Characterization and measurement of anthocyanins by UV-visible spectroscopy. Curr Protoc Food Anal Chem.

  6. Halliwell B, Gutteridge J, Cross CE (1992) Free radicals, anti-oxidants, and human disease: where are we now? J Lab Clin Med 119(6):598–620

  7. Jain P, Singh SK, Sharma HP, Basri F (2014a) Phytochemical screening and anti-fungal activity of Semecarpus anacardium L (an anti-cancer plant). Int J Pharm Sci Res 5(5):1884–1891

  8. Jain P, Sharma HP, Basri F, Baraik B, Kumari S, Pathak C (2014b) Pharmacological profiles of ethno-medicinal plant: Plumbago zeylanica L.—a review. Int J Pharm Sci Rev Res 24:157–163

  9. Jain P, Sharma HP, Basri F, Priya K, Singh P (2017) Phytochemical analysis of Bacopa monnieri (L) Wettst and their anti-fungal activities. Ind J Trad Knowl (IJTK) 16(2):310–318

  10. John BIJU, Sulaiman CT, George S, Reddy VRK (2014) Spectrophotometric estimation of total alkaloids in selected Justicia species. Int J Pharm Pharm Sci 6(5):647–648

  11. Khatiwora E, Adsul VB, Kulkarni MM, Deshpande NR, Kashalkar RV (2010) Spectroscopic determination of total phenol and flavonoid contents of Ipomoea carnea. Int J ChemTech Res 2(3):1698–1701


  13. Pezzuto JM (1997) Plant-derived anti-cancer agents. Biochem Pharmacol 53(2):121–133

  14. Sannigrahi S, Mazuder UK, Pal DK, Parida S, Jain S (2010) Anti-oxidant potential of crude extract and different fractions of Enhydra fluctuans Lour. Iran J Pharm Res IJPR 9(1):75–82

  15. Saxena V, Mishra G, Vishwakarma KK, Saxena A (2013) A comparative study on quantitative estimation of tannins in Terminalia chebula, Terminalia belerica, Terminalia arjuna and Saraca indica using spectrophotometer. Asian J Pharmaceutical Clin Res 6(3):148–149

  16. Sharma OP, Bhat TK (2009) DPPH antioxidant assay revisited. Food Chem 113(4):1202–1205



We are thankful to Head and Dean University Department of Botany, Ranchi University, Ranchi and AIRF, JNU, New Delhi for providing all necessary facilities. We also acknowledge UGC for providing financial assistance under Major Research Project F. No. 42-955/2013 (SR).

Author Information

Paras Jain
Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, India
Pallavi Singh
Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, India