Keywords: Medicinal properties, Inhibitory activities, Natural compounds, Free radicals, GC–MS analysis
Free radicals play a major role in development of many human diseases like cancer etc. In the present investigation Plumbago zeylanica (Pz) was used for anti-fungal activity, anti-oxidant activity as well as phytochemicals investigation. Anti-fungal activities were determined against four pathogenic fungal species Fusarium oxysporum, Rhizoctonia solanii, Alternaria sp. and Sclerotium rolfsii. Anti-oxidant activity and phytochemical investigation were determined by standard method using spectrophotometer, gas chromatography and mass spectroscopy. The excellent inhibitory activities were observed against Alternaria spp. (98%), F. oxysporum (68%), R. solanii (97.5%) followed by S. rolfsii (93.4%) at 62.5 μg/ml. The P. zeylanica showed free radicals scavenging activity by DPPH method (77.8% inhibition at 1000 µg/ml), CUPRAC assay (0.07940 TE mM at 1000 µg/ml), Anthocyanin content (2.404 CGE mg/ml), Flavonoid content (55,330 GAE/g), Phenols (14.75 µg/ml), Alkaloids (54 mg/g), Saponins (3.95%) and Tannin (7 µg/10 ml). So P. zeylanica extracts can be used as a source of bio-fungicide and scavenger of free radicals.
We are thankful to Head and Dean University Department of Botany, Ranchi University, Ranchi and AIRF, JNU, New Delhi for providing all necessary facilities. We also acknowledge UGC for providing financial assistance under Major Research Project F. No. 42-955/2013 (SR).