Research Articles | Published: 06 February, 2020
DOI: 10.1007/s42535-020-00102-z
First Page: 247
Last Page: 257
Views: 3637
Keywords: Medicinal properties, Inhibitory activities, Natural compounds, Free radicals, GC–MS analysis
Abstract
Free radicals play a major role in development of many human diseases like cancer etc. In the present investigation Plumbago zeylanica (Pz) was used for anti-fungal activity, anti-oxidant activity as well as phytochemicals investigation. Anti-fungal activities were determined against four pathogenic fungal species Fusarium oxysporum, Rhizoctonia solanii, Alternaria sp. and Sclerotium rolfsii. Anti-oxidant activity and phytochemical investigation were determined by standard method using spectrophotometer, gas chromatography and mass spectroscopy. The excellent inhibitory activities were observed against Alternaria spp. (98%), F. oxysporum (68%), R. solanii (97.5%) followed by S. rolfsii (93.4%) at 62.5 μg/ml. The P. zeylanica showed free radicals scavenging activity by DPPH method (77.8% inhibition at 1000 µg/ml), CUPRAC assay (0.07940 TE mM at 1000 µg/ml), Anthocyanin content (2.404 CGE mg/ml), Flavonoid content (55,330 GAE/g), Phenols (14.75 µg/ml), Alkaloids (54 mg/g), Saponins (3.95%) and Tannin (7 µg/10 ml). So P. zeylanica extracts can be used as a source of bio-fungicide and scavenger of free radicals.
References
- Abidemi OO (2013) Phytochemicals and spectrophotometric determination of metals in various medicinal plants in Nigeria. Int J Eng Sci Invent 2(5):51–54
- Assefa AD, Keum YS (2017) Effect of extraction solvent and various drying methods on polyphenol content and antioxidant activities of yuzu (Citrus junos Sieb ex Tanaka). J Food Meas Charact 11(2):576–585
- Banu S, Arunachalam G, Jayaveera KN, Ashoka Babu VL, Premakumari KB (2011) Estimation of total phenolic content and in vitro anti-oxidant activity of Barleria montana. Der Pharm Lett 3(4):178
- Diplock AT, Charuleux JL, Crozier-Willi G, Kok FJ, Rice-Evans C, Roberfroid M, Vina-Ribes J (1998) Functional food science and defence against reactive oxidative species. Br J Nutr 80(1):77–112
- Giusti MM, Wrolstad RE (2001) Characterization and measurement of anthocyanins by UV-visible spectroscopy. Curr Protoc Food Anal Chem. https://doi.org/10.1002/0471142913.faf0102s00
- Halliwell B, Gutteridge J, Cross CE (1992) Free radicals, anti-oxidants, and human disease: where are we now? J Lab Clin Med 119(6):598–620
- Jain P, Singh SK, Sharma HP, Basri F (2014a) Phytochemical screening and anti-fungal activity of Semecarpus anacardium L (an anti-cancer plant). Int J Pharm Sci Res 5(5):1884–1891
- Jain P, Sharma HP, Basri F, Baraik B, Kumari S, Pathak C (2014b) Pharmacological profiles of ethno-medicinal plant: Plumbago zeylanica L.—a review. Int J Pharm Sci Rev Res 24:157–163
- Jain P, Sharma HP, Basri F, Priya K, Singh P (2017) Phytochemical analysis of Bacopa monnieri (L) Wettst and their anti-fungal activities. Ind J Trad Knowl (IJTK) 16(2):310–318
- John BIJU, Sulaiman CT, George S, Reddy VRK (2014) Spectrophotometric estimation of total alkaloids in selected Justicia species. Int J Pharm Pharm Sci 6(5):647–648
- Khatiwora E, Adsul VB, Kulkarni MM, Deshpande NR, Kashalkar RV (2010) Spectroscopic determination of total phenol and flavonoid contents of Ipomoea carnea. Int J ChemTech Res 2(3):1698–1701
-
- Pezzuto JM (1997) Plant-derived anti-cancer agents. Biochem Pharmacol 53(2):121–133
- Sannigrahi S, Mazuder UK, Pal DK, Parida S, Jain S (2010) Anti-oxidant potential of crude extract and different fractions of Enhydra fluctuans Lour. Iran J Pharm Res IJPR 9(1):75–82
- Saxena V, Mishra G, Vishwakarma KK, Saxena A (2013) A comparative study on quantitative estimation of tannins in Terminalia chebula, Terminalia belerica, Terminalia arjuna and Saraca indica using spectrophotometer. Asian J Pharmaceutical Clin Res 6(3):148–149
- Sharma OP, Bhat TK (2009) DPPH antioxidant assay revisited. Food Chem 113(4):1202–1205
Author Information
Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, India