Assessment of in vitro anti-inflammatory, antioxidant and antidiabetic activities of Solanum khasianum Clarke

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00410-6
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Keywords: Solanum khasianum , Pharmacological property, HRBC membrane stabilization, Free radical scavenging, α-Amylase inhibition, α-Glucosidase inhibition


Abstract


Solanum khasianum, is a traditional medicinal plant of Solanaceae family. The plant represents a source of varied phytochemicals and was reported to be used in the treatment of several diseases. Therefore, our study was carried out to reveal anti-inflammatory, antioxidant and antidiabetic activities in different plant parts of S. khasianum. The leaf, fruit and root methanolic extracts of S. khasianum were investigated for its in vitro anti-inflammatory activity by Human Red Blood Cell (HRBC) membrane stabilization method; while DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, H2O2 radical scavenging and reducing power assays were utilized to determine antioxidant activity. Additionally, the antidiabetic activity was determined by α-amylase and α-glucosidase inhibition assay. Our results showed that the root methanolic extract of S. khasianum exhibited a high anti-inflammatory, antioxidant and antidiabetic activities in a dose-dependent manner. The S. khasianum root extract revealed a significant anti-inflammatory activity (83.97 ± 0.36%), potent antioxidant effect by DPPH radical scavenging assay (81.88 ± 0.58%), H2O2 radical scavenging assay (80.17 ± 0.40%) and reducing power assay (0.351 ± 0.62); and in addition, showed promising antidiabetic potential by inhibiting α-amylase (84.29 ± 0.89%) and α-glucosidase (85.02 ± 0.95%). These results further substantiated the claim and use of S. khasianum in traditional medicine for the treatment of inflammation, oxidative stress and diabetes mellitus.



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Acknowledgements


The authors acknowledge Department of Biotechnology, Kakatiya University, Warangal for providing us the research facility to conduct the research work.


Author Information


Chirumamilla Pavani
Department of Biotechnology, Kakatiya University, Warangal Dist, India

Taduri Shasthree
Department of Biotechnology, Kakatiya University, Warangal Dist, India
tadurishasthree@kakatiya.ac.in