Vegetos

Abstract

For testing seed purity and trueness, GOT (Grow Out Test) is a long term process and therefore, present study was carried out during Rabi-2011. The experimental materials used in the study comprised of four parents viz., Green Long (GL), Pusa Purple long (PPL), IBWL-2007-1 (IBWL), Punjab Sadabahar (PS) and their six F₁’s and standard check PH-6. RAPD marker was used to test the hybridity. Out of twenty-nine, only four primers showed polymorphism. namely OPA -14, OPA-08, OPH-01 and OPH-20. According to banding pattern for the primer OPH-01, hybrid between P4 and P₂ (IBWL-2007-1 X Green Long) resembles to male parent i.e., Green Long (P₂) and in same reciprocal cross P₂ and P₄ (Green Long X IBWL-2007-1), the banding patterns of F₁ hybrid resembles male parent i.e. IBWL-2007-1 (P₄). In another hybrid, between P₃ and P₄ (Punjab Sadabahar X IBWL-2007-1) banding pattern of F₁ hybrid resembles to male parent i.e., IBWL-20071 (P₄). For the primer OPH-20, F₁ hybrid between P₁ and P₃ (Punjab Sadabahar X IBWL-2007-1) banding pattern of F₁ hybrid resembles to male parent i.e. IBWL-2007-1 (P₃).For the primer OPA14, F₁ hybrid between P₃ and P₄ (IBWL-2007-1 X Pusa Purple Long) banding pattern of F₁ hybrid resembles to male parent i.e. Pusa Purple Long (P₄) and in the same reciprocal cross P₄ and P₃ (Pusa Purple Long X IBWL-2007-1) banding pattern of F₁ hybrid resembles to male parent i.e. IBWL-2007-1 (P₃). For primer OPA-18, in F₁ hybrid between P₁ and P₃ (Punjab Sadabahar X IBWL2007-1), the banding pattern resembles to male parent i.e. IBWL-2007-1 (P₃).