Biosynthesis of silver nanoparticles using mushroom extract and its toxicity assessement in zebrafish embryos

, ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00483-3
First Page: 119
Last Page: 126
Views: 417


Keywords: AgNP, Zebrafish, P. ostreatus , FET, SEM, FTIR


Abstract


The study undertakes the synthesis of “Silver Nanoparticles” (AgNPs) via biological approach in order to overcome the toxic effects caused by synthesis using conventional chemical methods by conducting in vivo toxicity in zebrafish embryos. Edible oyster mushroom P. ostreatus were utilized for synthesizing AgNPs in aqueous phase. 1 mM silver nitrate when combined with the prepared aqueous extract of P. ostreatus, causes reduction of silver nitrate to form nanoparticles of silver which were characterized using UV–VIS Spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infra-Red study (FTIR) techniques. In vivo toxicity assessment was carried out as per OECD guidelines for Fish Embryo Toxicity (FET). Study showed dose-dependent toxicity to different concentrations of biosynthesized AgNPs on exposure to zebrafish embryos and LC50 value was determined to be 1.8 mg/L.


AgNP, Zebrafish, 
                P. ostreatus
              , FET, SEM, FTIR


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf, B 28(4):313–318


Al-Bahrani R, Raman J, Lakshmanan H, Hassan AA, Sabaratnam V (2017) Green synthesis of silver nanoparticles using tree oyster mushroom Pleurotus ostreatus and its inhibitory activity against pathogenic bacteria. Mater Lett 1(186):21–25


Chan DW, Yager TD (1998) Preparation and imaging of nuclear spreads from cells of the zebrafish embryo. Chromosoma 107(1):39–60


Chowdhury MM, Kubra K, Ahmed SR (2015) Screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in Bangladesh. Ann Clin Microbiol Antimicrob 14(1):1–6


Ibrahim HMM (2015) Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. J Radiat Res Appl Sci 8:265–275. https://doi.org/10.1016/j.jrras.2015.01.007


Irshad A, Sarwar N, Sadia H, Malik K, Javed I, Irshad A, Afzal M, Abbas M, Rizvi H (2020) Comprehensive facts on dynamic antimicrobial properties of polysaccharides and biomolecules-silver nanoparticle conjugate. Int J Biol Macromol 15(145):189–196


Martínez-Flores HE, Contreras-Chávez R, Garnica-Romo MG (2020) Effect of extraction processes on bioactive compounds from Pleurotus ostreatus and Pleurotus djamor: their applications in the synthesis of silver nanoparticles. J Inorg Organomet Polym Mater 20:1–3


Parng C, Roy NM, Ton C, Lin Y, McGrath P (2007) Neurotoxicity assessment using zebrafish. J Pharmacol Toxicol Methods 55(1):103–112


Piska K, Sułkowska-Ziaja K, Muszyńska B (2017) Edible mushroom Pleurotus ostreatus (oyster mushroom): its dietary significance and biological activity. Acta Scientiarum Polonorum. Hortorum Cultus 16(1):151–161


Polte J (2015) Fundamental growth principles of colloidal metal nanoparticles–a new perspective. CrystEngComm 17(36):6809–6830


Raman J, Jang KY, Oh YL, Oh M, Im JH, Lakshmanan H, Sabaratnam V (2021) Cultivation and nutritional value of prominent Pleurotus spp.: an overview. Mycobiology 49(1):1–4


Xia G, Liu T, Wang Z, Hou Y, Dong L, Zhu J, Qi J (2016) The effect of silver nanoparticles on zebrafish embryonic development and toxicology. Artif Cells Nanomed Biotechnol 44(4):1116–1121

 


Acknowledgements


We would like to acknowledge MGMSBS & MGM Institute of Health Sciences for permitting us to conduct this study. We also like to acknowledge MGM’s Institutional Animal Ethical Committee for critically assessing the project and for continuous monitoring of the project.


Author Information


Jadhav Sarvesh R.
Department of Medical Biotechnology, MGM School of Biomedical Sciences, MGMIHS, Kamothe, India
sarveshjadhav16@gmail.com
Thakur Mansee
Department of Medical Biotechnology, MGM School of Biomedical Sciences, MGMIHS, Kamothe, India
mansibiotech79@gmail.com

Gupta Himanshu
Department of Medical Biotechnology, MGM School of Biomedical Sciences, MGMIHS, Kamothe, India

dr.himanshugupta@mgmsbsnm.edu.