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Monalisa Kumari, Justine Angima Kibari, Kar Swayangprava, Kingsley Angima Bichang’a, Behera Shashikanta
Keywords: Elicitation and precursor feeding, Hairy root culture, Micropropagation, Plumbagin, n Plumbago zeylanican
Plumbago zeylanica L. is an important medicinal herb belonging to the family Plumbaginacea. Since time immemorial, it has been used for the treatment of various ailments. This plant also possesses various pharmacological activities, including hepatoprotective, anticancer, antifertility, antidiabetic, neuroprotective, and cardio-protective. The above-mentioned pharmacological activities of this plant are due to the presence of a wide range of secondary metabolites, including plumbagin. This plant is conventionally propagated using seeds, which have its own drawbacks, including low seed germination, short-term viability, and infestation by pests and insects. Thus, the conventional propagation method is unable to produce a large number of plants to meet their commercial demand. This challenge can be alleviated by the use of different biotechnological approaches. Plant tissue culture is an alternative tool for large scale production of in vitro plants and pharmaceutically important secondary metabolites (plumbagin) within a short span of time to narrow down the industrial demand. This review highlights the present status of P. zeylanica, it’s challenges in regard to over exploitation and how to amend this. Further, we have also focused on the production of plumbagin content using various approaches.
Azad AK, Praveen M, Sulaiman WM (2024) Assessment of anticancer properties of Plumbago zeylanica. In: Roy A (ed) Harnessing medicinal plants in cancer prevention and treatment. IGI Global, New York, pp 91–121
Badwaik HR, Kumari L, Nakhate K, Verma VS, Sakure K (2019) Phytoconstituent plumbagin: chemical, biotechnological and pharmaceutical aspects. Stud Nat Prod Chem 63:415–460
Barathkumar TR (2019) Studies on influence of different seed treatments on dormancy breaking in aonla (Phyllanthus emblica L.). J Pharm Phytochem SP2:131–133
Basu A, Joshi RK, Jha S (2015) Genetic transformation of Plumbago zeylanica with Agrobacterium rhizogenes strain LBA 9402 and characterization of transformed root lines. Plant Tissue Cult Biotechnol 25(1):21–35
Bavi K, Khavari-Nejad RA, Najafi F, Ghanati F (2022) Phenolics and terpenoids change in response to yeast extract and chitosan elicitation in Zataria multiflora cell suspension culture. 3 Biotech 12:163. https://doi.org/10.1007/s13205-022-03235-x
Behera S, Nayak N, Shasmita BDP, Naik SK (2015) A novel and efficient micropropagation protocol of Bacopa monnieri (L.) Pennell through two-stage culture of nodal segments and ex vitro acclimatization. J Appl Biol Biotechnol 3(3):16–21
Behera S, Kamila PK, Rout KK, Barik DP, Panda PC, Naik SK (2018) An efficient plant regeneration protocol of an industrially important plant, Hedychium coronarium J. Koenig and establishment of genetic & biochemical fidelity of the regenerants. Ind Crop Prod 126:58–68
Behera S, Kar SK, Rout KK, Barik DP, Panda PC, Naik SK (2019a) Assessment of genetic and biochemical fidelity of field-established Hedychium coronarium J. Koenig regenerated from axenic cotyledonary node on meta-topolin supplemented medium. Ind Crops Prod 134:206–215
Behera S, Kar SK, Rout KK, Barik DP, Panda PC, Naik SK (2019b) Assessment of genetic and biochemical fidelity of field-established Hedychium coronarium J. Koenig regenerated from axenic cotyledonary node on meta-Topolin supplemented medium. Ind Crop Prod 134:206–215
Behera S, Rout KK, Panda PC, Naik SK (2020) Production of non-embryogenic synthetic seeds for propagation and germplasm transfer of Hedychium coronarium J Koenig. J Appl Res Med Arom Plant 19:100271
Behera S, Chouhan VBS, Pati K, Bansode VV, Nedunchezhiyan M, Verma AK, Monalisa K, Naik PK (2022a) Biology and biotechnological aspect of sweet potato (Ipomoea batatas L.) a commercially important tuber crop: a review. Planta 256:40
Behera S, Monalisa K, Meher RK, Mohapatra S, Das PK, Madkami SK, Naik PK, Naik SK (2022b) Phytochemical fidelity and therapeutic activity of micropropagated plant Curcuma amada Roxb: an important medicinal herb. Ind Crop Prod 176:11440
Behera S, Kar SK, Monalisa K, Mohapatra S, Meher RK, Rout KK, Barik DP, Panda PC, Naik PK, Naik SK (2023a) Assessment of genetic, biochemical fidelity and therapeutic activity of in vitro regenerated plant Hedychium coronarium J. Koenig. In Vitro Cell Develop Bio Plant. https://doi.org/10.1007/s11627-023-10383-z
Behera S, Mohapatra S, Meher RK, Das PK, Babu SK, Naik M, Monalisa K, Naik SK, Naik PK (2023b) Assessment of chemical composition and therapeutic activities of Clausena excavata Burn: an important medicinal plant of Eastern Ghats India. J Essen Oil Bear Plants 26(2):502–521
Behera S, Chouhan VBS, Monalisa K, Mehera RK, Kar SK, Pati K, Bansode VV, Nedunchezhiyan M, Verma AK, Naik SK, Naik PK (2024) In vitro plant regeneration, genetic and biochemical fidelity and anticancer activity of anthocyanin rich purple flesh sweet potato var. Bhu Krishna South Afr J Bot 166:332–343
Bloch K, Parihar VS, Kellomäki M, Ghosh S (2022) Natural compounds from Plumbago zeylanica as complementary and alternative medicine. In: Chakraborti S (ed) Handbook of oxidative stress in cancer: therapeutic aspects. Springer, Singapore. https://doi.org/10.1007/978-981-16-1247-333-1
Butiuc-Keul A, Farkas A, Cristea V (2016) Genetic stability assessment of in vitro plants by molecular markers. Stud UBB Biol 61(1):107–114
Chandravanshi M, Sahu Y, Agrawal A, Raja W (2014) In vitro micropropagation of important commercial medicinal plant: Plumbago zeylanica. Adv Biol Res 8(3):139–142
Chaplot BB, Dave AM, Jasrai YT (2006) A valued medicinal plant-Chitrak (Plumbago zeylanica Linn): successful plant regeneration through various explants and field performance. Plant Tissue Cult Biotechnol 16(2):77–84
Chowdhury S, Shuvo MJM, Mannan MA (2023) A potential review of its traditional uses, phytochemistry, pharmacology, and toxicity of Plumbago zeylanica. J Phramacogn Phytochem 12(5):123–131
Das G, Rout GR (2002) Direct plant regeneration from leaf explants of Plumbago species. Plant Cell Tissue Org Cult 68:311–314
Dhama K, Sharun K, Gugjoo MB, Tiwari R, Alagawany M, Yatoo MI, Thakur P, Iqbal HMN, Chaicumpa W, Michalak I, Elnesr SS, Farag MR (2023) A comprehensive review on chemical profile and pharmacological activities of Ocimum basilicum. Food Rev Int 39(1):119–147
Folami SO, Abubakar MF, Lawal AS (2024) Effect of Plumbago zeylanica L. (Ceylon leadwort) methanol leaf extract on the hepatic profile of male Wistar rats. J Exp Lab Med 131(2):60–66
Giannenas I, Sidiropoulou E, Bonos E, Christaki E, Florou-Paneri P (2020) The history of herbs, medicinal and aromatic plants, and their extracts: past, current situation and future perspectives. In: Feed additives. Academic Press, London, , pp 1–18
Gorfad K, Desai BS, Jha SS, Patel DP, Prajapati VM, Garde YA (2022) Influence of different hormones and potting media on growth and quality of Chitrak [Plumbago zeylanica L.]. Pharma Innov J 11(11):2389–2394
Hassan AKMS, Haque F, Jahan MAA, Roy SK (2012) In vitro mass propagation of Plumbago zeylanica L. through direct organogenesis. Bangladesh J Sci Ind Res 47(3):297–302
Isah T, Umar S, Mujib A, Sharma MP, Rajasekharan PE, Zafar N, Frukh A (2018) Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield. Plant Cell Tiss Organ Cult 132:239–265
Jain AK, Bashir M (2011) An efficient regeneration and plantlet development protocol from somatic tissues of Plumbago zeylanica L. J Pharm Res 4(9):2860–2863
Jain P, Danwra K, Sharma HP, Mahato D (2018) In vitro tissue culture studies and synthetic seed formation from Plumbago zeylanica L. Indian J Exp Biol 56:769–773
Jaiswal A, Sabarwal A, Mishra JPN, Singh RP (2018) Plumbagin induces ROS-mediated apoptosis and cell cycle arrest and inhibits EMT in human cervical carcinoma cells. RSC Adv 8(56):32022–32037
Javed R, Yucesan B, Zia M, Gurel E (2022) Nanoelicitation: a promising and emerging technology for triggering the sustainable in vitro production of secondary metabolites in medicinal plants. In: Chen JT (ed) Plant and nanoparticles. Springer, Singapore, pp 265–280
Kakade PS, Zimare SB, Malpathak NP (2022) Effects of Sargassum ilicifolium seaweed extract on enhanced in vitro seed germination, mass propagation, and accumulation of plumbagin in Plumbago zeylanica L. Plnat Cell Tiss Org Cult 149:399–410
Kanungo S, Sahoo SL, Sahu RK (2012) Development of a simplified protocol for in vitro propagation of a valuable medicinal plant Plumbago zeylanica Linn. through nodal explants found in Odisha, India. J Med Plants Res 6(13):2627–2632
Karpagam N, Rao MV, Jeyachandran R (2006) Somatic embryogenesis and plant regeneration in Plumbago zeylanica L. through suspension culture. In: Ray JG, Joseph K, Varghese G (eds) Science and technology for sustainable development allied. Publisher Pvt Ltd, London
Katoch K, Gupta S, Nazir R, Kumar V, Sanyal R, Dey A, Pandey DK (2021) Establishment of adventitious root culture from leaf explants of Plumbago zeylanica: an endangerd medicinal plant. Vegetos 35:276–280
Katoch K, Gupta S, Gupta AP, Goyal P, Devi R, Dey A, Pandey DK (2022) Biotic elicitation for enhanced production of plumbagin in regenerated shoot cultures of Plumbagozeylanica using response surface methodology. Plant Cell Tissue Org Cult 151(3):605–615
Kaushal J, Rajbhar K, Dawda H, Mukundan U (2022) Quantification of leaf and root plumbagin in Plumbago zeylanica followed by a comparative study with callus and commercial source. J Adv Sci Res 13(2):202–206
Khatik N, Joshi R (2016) In vitro adventitious shoot regeneration from cotyledon and hypocotyl explants of Murraya koenigii (L.) spreng. J Phytol Res 29(1):7–16
Kiselev KV, Shumakova OA, Manyakhin AY (2013) Effect of plant stilbene precursors on the biosynthesis of resveratrol in Vitis amurensis Rupr. cell cultures. Appl Biochem Microbiol 49:53–58
Kumar V, Sharma N, Sood H, Chauhan RS (2016) Exogenous feeding of immediate precursors reveals synergistic effect on picroside-I biosynthesis in shoot cultures of Picrorhiza kurroa Royle ex Benth. Sci Rep 6:1–9
Kumar A, Kumari A, Demiwal P, Roy P, Sircar D (2023) Enhanced production of bioactive plumbagin in hairy root cultures and adventitious root cultures of Plumbago zeylanica L. by a novel apocarotenoid elicitor, α-ionone. Ind Crop Prod 203:117140
Laura E, Brambilla A, Vlot AC (2022) New molecules in plant defence against pathogens. Essays Biochem 66:683–693
Li T, Lv M, Chen X, Yu Y, Zang G, Tang Z (2019) Plumbagin inhibits proliferation and induces apoptosis of hepatocellular carcinoma by down regulating the expression of SIVA. Drug Des Devel Ther 2019:1289–1300. https://doi.org/10.2147/DDDT.S200610
Liu Y, Deng F, Liu C, Meng QY, Gao ZW (2006) Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC. China J Chin Mater Med 31(20):1684–1686
Lubaina AS, Nair GM, Murugan K (2011) Shoot multiplication and direct organogenesis of an important medicinal plant Plumbago zeylanica L. (Plumbaginaceae). J Res Biol 6:424–428
Majumder S, Rahman MM (2016) In vitro rapid clonal propagation of Plumbago zeylanica L. through direct organogenesis. Int J Adv Biotechnol Res 7(3):877–887
Mallikadevi T, Senthilkumar P, Paulsamy S (2008) In vitro regeneration of the medicinal plant, Plumbago zeylanica L. with reference to a unique population in Maruthamalai, the western ghats, India. Plant Tissue Cult Biotechnol 18(2):173–179
Monalisa K, Behera S, Pidika SP, Nial PS, Naik SK (2024) In vitro propagation and assessment of genetic fidelity of Blepharispermum subsessile DC.: an endangered medicinal plant of India. Vegetos. https://doi.org/10.1007/s42535-024-00851-1
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Padarthi PK, Swamy KS, Kumar P, Jallepalli VR, Deshpande M (2023) Phyto-pharmacological investigation of Plumbago zeylanica for memory enhancing activity. Int J Pharma Quality Assur 14(4):1023–1026
Pant M, Lal A, Rana S, Rani A (2012) Plumbago zeylanica L.: a mini review. Int J Pharm Appl 3:399–405
Rai MK, Rathour R, Behera S, Kaushik S, Naik SK (2021) Encapsulation technology: an assessment of its role in in vitro conservation of medicinal and threatened plant species, Chapter 5. In: Srivastava DK, Thakur AK, Kumar P (eds) Agricultural biotechnology: latest research and trends. Springer, London
Raja HD, Jenifer AM, Steffi PF, Thamilmaraiselvi B, Srinivasan P, Tamilvanan R (2018a) Micropropagation of Plumbago zeylanica—an important medicinal plant. World J Pharm Pharm Sci 7(4):1823–1829. https://doi.org/10.20959/wjpps20184-11467
Raja HD, Jenifer AM, Steffi PF, Thamilmaraiselvi B, Srinivasan P, Tamilvanan R (2018b) Micropropagation of Plumbago zeylanica—an important medicinal plant. World J Pharma Pharmaceut Sci 7(4):1823–1829
Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusidó RM, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21:182
Rency AS, Satish L, Pandian S, Rathinapriya P, Ramesh M (2016) In vitro propagation and genetic fidelity analysis of alginate-encapsulated Bacopa monnieri shoot tips using Gracilaria salicornia extracts. J Appl Phycol 29:481–494
Rout GR, Das G (2002) An assessment of genetic integrity of micropropagated plants of Plumbago zeylanica by RAPD markers. Biol Plant 45:27–32
Rout GR, Saxena C, Das P, Samantaray S (1999) Rapid clonal propagation of Plumbago zeylanica Linn. Plant Growth Regul 28:1–4
Rout GR, Das G, Samantaray S, Das P (2001) Micropropagation of Plumbago zeylanica L. by encapsulated nodal explants. J Horticult Sci Biotechnol 76(1):24–29
Roy A, Bharadvaja N (2019) Establishment of root suspension culture of Plumbago zeylanica and enhanced production of plumbagin. Ind Crop Prod 137:419–427
Sahoo S, Debata BK (1998) Micropropagation of Plumbago zeylanica Linn. J Herbs Spices Med Plants 5(4):87–93
Santra I, Ghosh B (2023) Sustainable production of plumbagin via meta-Topolin mediated high frequency regeneration of genetically true-to-type Plumbago zeylanica L. 3 Biotech 13:225
Santra I, Chakraborty A, Ghosh B (2024) Evaluation of plumbagin synthesis: mimicking in vivo plant systems through the application of elicitors inducing stress on in vitro regenerated Plumbago zeylanica L. Plant Cell Tissue Org Cult 157:1–16
Sariga KS, Harisha CR (2024) Comparative micrometric evaluation of leaves of rakta (Plumbago rosea Linn.) and sweta (Plumbago zeylanica Linn.) Chitraka. J Ayur 18(3):201–207
Selvakumar V, Anbudurai PR, Balakumar T (2001) In vitro propagation of the medicinal plant Plumbago zeylanica L. through nodal explants. In Vitro Cell Dev Biol Plant 37:280–284
Sharad KP, Nutan MP (2017) Micropropagation of Plumbago zeylanica L. using in vitro germinated seedling explants. Int J Life Sci 5(2):211–218
Sharma U, Agrawal V (2018) In vitro shoot regeneration and enhanced synthesis of plumbagin in root callus of Plumbago zeylanica L. an important medicinal herb. In Vitro Cell Dev Biol Plant 54:423–435. https://doi.org/10.1007/s11627-018-9889-y
Sharma A, Singh N (2015) A multifarious potent herb: Plumbago zeylanica—a mini review. Int J Recent Sci Res 6(6):4825–4829
Sharma P, Dwivedee BP, Bisht D, Dash AK, Kumar D (2019) The chemical constituents and diverse pharmacological importance of Tinospora cordifolia. Heliyon 5(9):e02437
Shasmita SNR, Rath SK, Behera S, Naik SK (2018) In vitro secondary metabolite production through fungal elicitation: an approach for sustainability. In: Prasad R (ed) Fungal nanobionics: principles and applications. Springer, London
Shasmita BS, Mishra P, Samal M, Mohapatra D, Monalisa K, Naik SK (2023) Recent advances in tissue culture and secondary metabolite production in Hypericum perforatum L. Plant Cell Tissue Org Cult 154:13–28
Shukla B, Saxena S, Usmani S, Kushwaha P (2021) Phytochemistry and pharmacological studies of Plumbago zeylanica L.: a medicinal plant review. Clin Phytosci 7:1–11
Singh T, Sharma U, Agrawal V (2020) Isolation and optimization of plumbagin production in root callus of Plumbago zeylanica L. augmented with chitosan and yeast extract. Ind Crop Prod 151:112446
Sivanesan I (2007) Shoot regeneration and somaclonal variation from leaf callus cultures of Plumbago zeylanica Linn. Asian J Plant Sci 6:83–86
Sivanesan I, Jeong BR (2009a) Induction and establishment of adventitious and hairy root cultures of Plumbago zeylanica L. Afr J Biotechnol 8(20):120–127
Sivanesan I, Jeong BR (2009b) Micropropagation of Plumbago zeylanica L. Afr J Biotechnol 8(16):112–119
Sundari BKR, Telapolu S, Dwarakanath BS, Thyagarajan SP (2017) Cytotoxic and antioxidant effects in various tissue extracts of Plumbago zeylanica: implications for anticancer potential. Pharmacogn J. https://doi.org/10.5530/pj.2017.5.111
Swieca M (2016) Potentially bioaccessible phenolics, antioxidant activity and nutritional quality of young buckwheat sprouts affected by elicitation and elicitation supported by phenylpropanoid pathway precursor feeding. Food Chem 192:625–632
Tripathi MK, Tiwari G, Tiwari S, Tripathi N, Sharma M, Bhargav S, Patidar SL, Tiwari S (2022) Influence of plant growth regulators on in vitro morphogenesis in PlumbagoZeylanica Linn. Res Aspect Biol Sci 7(7):96–123
Verma PC, Singh D, Rahman L, Gupta MM, Banerjee S (2002) In vitro-studies in Plumbago zeylanica: rapid micropropagation and establishment of higher plumbagin yielding hairy root cultures. J Plant Physiol 159(5):547–552
Waman AA, Bohra P, Sathyanarayana BN, Umesha K, Mukunda GK, Ashok TH, Gowda B (2015) Optimization of factors affecting in vitro establishment, ex vitro rooting and hardening for commercial scale multiplication of silk banana (Musa AAB). Erwerbs-Obstbau 57(3):153–164
Weathers PJ, Arsenault PR, Covello PS, McMickle A, Teoh KT, Reed DW (2011) Artemisinin production in Artemisia annua: studies in planta and results of a novel delivery method for treating malaria and other neglected diseases. Phytochem Rev 10(2):173–183
Wei X, Gou X, Yuan T, Russell SD (2006) A highly efficient in vitro plant regeneration system and Agrobacterium-mediated transformation in Plumbago zeylanica. Plant Cell Rep 25:513–521
Department of Botany, Ravenshaw University, Cuttack, India