Biotechnology based strategies for secondary metabolites enhancement: a review

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Review Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00647-9
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Keywords: In vitro techniques, Medicinal plant, Plant tissue culture, Secondary metabolites, Suspension culture


The primary sources of essential secondary metabolites (SMs) which are crucial for drug discovery and development are derived from plants. Plants frequently utilize these secondary metabolites for defense against herbivore, between two different species, and resistance to diverse stresses. Humans are dependent on these secondary metabolites for medicines, color, flavor, and drug production. Today pharmaceutical industries heavily rely on herbal remedies, which eventually drive plants out of existence. In this review, different plant cell culture and modern techniques has been introduced to study the effective and enhanced production of useful secondary metabolites. Numerous in vitro techniques have been discussed, such as elicitation, the hairy root culture, organ culture, suspension culture, etc., which are used in the production of SMs. Special emphasis has been given on plant tissue culture techniques (PTCs). Although hairy root culture and other organ cultures proven to be effective in meeting the need for natural products, however suspension culture and elicitation are the primary methods utilized to increase SM production. The processes that result in the secondary metabolites production by plants are now simple to control and handle. The production of medicinally relevant SMs can be facilitated by methods like plant cell, tissue, and organ cultures. Most in vitro approaches are now been employed as a result of knowledge of the SM pathway in commercially relevant plants. It is possible that in the future, advancements will allow the effective extraction of important and unknown molecules from medicinal plants, which can be utilized as renewable sources for vital components in medicine.

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The authors would like to acknowledge constant support received by Delhi Technological University, Delhi.

Author Information

Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India

Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India
Bharadvaja Navneeta
Plant biotechnology laboratory, Department of biotechnology, Delhi technological university, Delhi, India