Characterization of culture condition dependent, growth responses of phosphate solubilizing bacteria (Bacillus subtilis DR2) on plant growth promotion of Hordeum vulgare

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00589-2
First Page: 266
Last Page: 276
Views: 1144


Keywords: Rhizosphere, Phosphate solubilization, 16S rRNA gene sequence, n Bacillus subtilisn , Barley seedlings


Abstract


Phosphorus (P) is one of the essential macro nutrients required for the growth and development of plant. Phosphate solubilising bacteria (PSB) are very effective in improving soil fertility by solubilising insoluble soil P, making them readily available to the plants. In the present work, PSB were isolated from the rhizosphere of Eragrostis cynosuroides on Pikovskaya’s agar media at 30 °C and pH 7. Among positive isolates, the isolate DR2 was selected, based on highest zone of solubilization (15 mm), phosphate solubilization efficiency (150%) and solubilization index (4.5) on 4th day of incubation period. The isolated DR2 was identified as Bacillus subtilis on the basis 16 S rRNA gene sequence (Gene bank Accession Number KP455653). Optimized cultural conditions resulted in maximum P-solubilization after 96 h of incubation at 35 °C in Pikovsakaya’s broth (having 1% TCP) of pH 7.0 with glucose and ammonium sulphate, used as carbon and nitrogen sources, respectively. B. subtilis DR2 significantly enhanced the growth of barley seedlings in terms of seed germination (60%) with percent enhancements in root length (93.71%), shoot length (41.30%) and biomass (22.44%), over control. The strain B. subtilis DR2 can be used as a biofertilizer for a sustainable integrated plant nutrient supply (IPNS) system.


Rhizosphere, Phosphate solubilization, 16S rRNA gene sequence, n              Bacillus subtilisn            , Barley seedlings


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Acknowledgements


The authors acknowledge the support of the Head, Department of Botany, Patna University, Patna, for providing the necessary facilities for completion of this work. The authors are also thankful to Xcelris, India for the molecular confirmation of our isolates by 16 S rRNA gene sequencing.


Author Information


Kumari Sonali
Microbial Bioaffiliationersity Lab, Department of Botany, Patna University, Patna, India
ksonali.mic@gmail.com
Kumar Pankaj
Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, India
guptapankaj23@gmail.com

Kiran Shilpi
Department of Botany, Patna Women’s College, Patna University, Patna, India


Kumari Sushma
Microbial Bioaffiliationersity Lab, Department of Botany, Patna University, Patna, India


Singh Abha
Microbial Bioaffiliationersity Lab, Department of Botany, Patna University, Patna, India