Vegetos
(An International Journal of Plant Research & Biotechnology)
Characterization of silver nanoparticles from endophytic fungi Mucor circinelloides and its antimicrobial and anticancer effect
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Research Articles | Published: 31 October, 2025
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Keywords: Antimicrobial activity, AgNP’s, n Mucor circinelloidesn , SEM, TEM, Cytotoxic study
Abstract
In recent years silver nanoparticles derived from endophytic fungi are gaining considerable importance due to their medicinal properties. Endophytic fungi Mucor circinelloides was selected for biosynthesis of silver nanoparticles and evaluated for their antimicrobial and anticancer properties. The nanoparticles were extracted and characterized by UV–visible spectroscopy, Transmission electron microscopy, Scanning electron microscopy, and Fourier transmission infrared microscopy analysis. UV–visible spectra showed SPR band peak at 440–442 nm, which confirmed the presence of nanoparticles. FTIR spectroscopy results reveal the presence of organic compounds like aldehydes, amines, and alkenes, which play significant roles in stabilizing silver nanoparticles. Transmission electron microscopy and SEM images reveal the size and shape of nanoparticles range from 10 to 20 nm. Antibacterial studies were performed against staphylococcus aureus, Escherichia coli, Salmonella typhi, Micrococcus luteus, and Proteus vulgaris through the disk diffusion method. Ethyl acetate extract showed considerable antibacterial activity against salmonella typhi and E. coli. In anticancer studies, the nanoparticles showed remarkable anticancer effects on the MCF-7 and HCT-116 cell lines. The IC50 value observed for these cell lines was 5.47 µg/ml and 14.62 µg/ml, respectively at 72 h of incubation through MTT assay.
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Author Information
Department of Microbiology, Kakatiya University, Hanamkonda, India