Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Comparative genetic diversity assessment of chickpea wilt fungus, Fusarium oxysporum f. sp. ciceris using SCoT and SRAP markers
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Mullachery Sruthy, Mane Shyamsunder S., Ingle Sunil T., Jadhav Pravin V.
Research Articles | Published: 30 June, 2026
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Keywords: Bioinformatics, Chickpea wilt, Genetic diversity, SCoT, SNP and SRAP
Abstract
The dynamics of shift in pathogen population can be determined by analysing the degree of gene flow and genetic divergence. The diversity of sixteen single spore isolates of Fusarium oxysporum f. sp. ciceris (Foc) collected from eight different states of India such as Maharashtra(2), Uttar Pradesh(2), Madhya Pradesh(2), Haryana(2), Gujarat(2), Rajasthan(2), Andhra Pradesh(2) and Karnataka(2) were assessed using twenty five SCoT (Start Codon Target) and thirty SRAP (Sequence Related Amplified Polymorphism) markers. Mapping of isolates clustered them according to their geographical origin, which indicated role of geographical area in genetic diversity. Although, both SCoT and SRAP were found to exhibit comparable results in marker and genetic diversity parameters analysis, SRAP (PIC-0.40; polymorphism-86.56%) stands out as a bit superior than SCoT (PIC-0.37; polymorphism-84.70%). Mapping of isolates through dendrograms and PCoA clustered them according to their geographical origin, indicating that regional environmental factors strongly influence genetic diversity. Moderate degree of genetic differentiation (Gst:0.08) and significant gene flow (Nm: 5.75) were observed, suggesting pathogen’s inherent ability to evolve under environmental stress and overcome host resistance. Bioinformatics study of monomorphic allele sequences showed Single Nucleotide Polymorphism (SNP) among them and all coded for an extra cellular serine rich protein.
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Author Information
Department of Plant Pathology, Akola, India