Cultivation and medicinal properties of wild edible Pleurotus ostreatus of Tripura, Northeast India

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-019-00035-2
First Page: 238
Last Page: 246
Views: 750

Keywords: Biological efficiency, EC50 , Fruit body, Mycelium, Productivity


Cultivation of edible mushrooms is one of the most economically usable processes for bioconversion of agro wastes for the production of protein-rich food with various medicinal values. The present investigation was focused to determine the most effective and suitable substrate for the cultivation of Pleurotus ostreatus considering biological efficiency and medicinal activities of fruiting bodies. Different parameters like mycelium running rate, development of fruiting bodies, cropping phase, productivity, biological efficiency and medicinal activities like antibacterial, antioxidant activities of this mushroom were evaluated. The total mycelium running, total primordial formation, complete fruit body formation and duration of cropping were dominant in PS substrate. Fruiting body size was bigger in SD substrate but higher productivity (19.6%) and biological efficiency (65.33%) was observed in PS substrate. Methanolic extracts of cultivated P. ostreatus on PS substrate showed higher antibacterial, free radical scavenging activity (74.26%), chelating effects on ferrous ion (91.11%), total phenol content (0.097 mg GAE/g) and flavonoids content (0.017 mg CE/g). The lowest EC50 values of cultivated P. ostreatus were found in PS substrate which indicated the stronger ability of antioxidant activity. The present study indicates that the types of used substrates affect biological efficiency and medicinal properties.

Biological efficiency, EC50
                     , Fruit body, Mycelium, Productivity

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  1. Alam N, Amin R, Khair A, Lee TS (2010) Influence of different supplements on the commercial cultivation of milky white mushroom. Mycobiology 38(3):184–188.

  2. Al-Momany A, Ananbeh K (2010) Conversion of Agricultural wastes into value added product with high protein content by growing Pleurotus ostreatus. In: Gökçekus H, Türker U, LaMoreaux J (eds) Survival and sustainability. Environmental earth sciences. Springer, Berlin, pp 1483–1490.

  3. Amin SM, Rahman MM, Hossain MM, Haque MM, Sarker NC (2007) Effect of different substrates on the growth and yield of five selected oyster mushrooms. Bangladesh J Mushroom 1(2):21–25

  4. Ananbeh K, Almomany A (2008) Production of Oyster mushroom (Pleurotus ostreatus) on tomato tuff agrowaste. Dirasat Agric Sci 35:133–138

  5. Andrade MCN, Zied DC, Minhoni MTA, Filho JK (2008) Yield of four Agaricus bisporus strains in three compost formulations and chemical composition analyses of the mushrooms. Braz J Microbiol 39(3):593–598.

  6. Arbaayah HH, Umi Kalsom Y (2013) Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and split gill mushroom (Schizophyllum commune) ethanolic extracts. Mycosphere 4(4):661–673.

  7. Bauer AW, Kirby WMM, Sherries JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45(4):493–496.

  8. Chang ST, Lau OW, Cho KY (1981) The cultivation and nutritive value of Pleurotus sojar-caju. Eur J Appl Microbiol Biotechnol 12:58.

  9. Chowdhury MMH, Kubra K, Ahmed SR (2015) Screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in Bangladesh. Ann Clin Microbiol Antimicrob 14(8):1–6.

  10. Cohen R, Persky L, Hadar Y (2002) Biotechnological applications and potential of wood degrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol 58(5):582–594.

  11. Debnath S, Upadhyay RC, Das P, Saha AK (2017) Antioxidant activities of methanolic extracts from ten Pleurotus species. Int Res J Pharm 8(3):44–49.

  12. Decker EA, Welch B (1990) Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem 38:674–677.

  13. Deepalakshmi K, Mirunalini S (2014) Assessment of in vitro antioxidant and antimicrobial properties of cultivated Pleurotus ostreatus: an edible mushroom. Free Radic Antioxid 4(2):27–32.

  14. Duh PD, Tu YY, Yen GC (1999) Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT Food Sci Technol 32(5):269–277.

  15. Fanadzo M, Zireva DT, Dube E, Mashingaidze AB (2010) Evaluation of various substrates and supplements for biological efficiency of Pleurotus sajor-caju and Pleurotus ostreatus. Afr J Biotechnol 9(19):2756–2761.

  16. Ferreira ICFR, Barros L, Abreu RMV (2009) Antioxidants in wild mushrooms. Curr Med Chem 16(12):1543–1560.

  17. Gasecka M, Mleczek M, Siwulski M, Niedzielski P (2016) Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc. Eur Food Res Technol 242(5):723–732.

  18. Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir-KneźEvić S (2004) Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm 54(1):65–72

  19. Lennox JA, Abriba C, Alabi BN, Akubuenyi FC (2010) Comparative degradation of sawdust by microorganisms isolated from it. Afr J Microbiol Res 4(17):1804–1807

  20. Mandeel QA, Al-Laith AA, Mohamad SA (2005) Cultivation of oyster mushrooms (Pleurotus spp) on various lignocellulosic wastes. World J Microbiol Biotechnol 21(4):601–607.

  21. Mau LJ, Huang PN, Hung SJ, Chen CC (2004) Antioxidant property of methanolic extracts from two kinds of Antrodia camphorata mycelia. Food Chem 86(1):25–31.

  22. Michael HW, Bultosa G, Pant LM (2011) Nutritional contents of three edible oyster mushrooms grown on two substrates at Haramaya, Ethiopia, and sensory properties of boiled mushroom and mushroom sauce. Int J Food Sci Technol 46(4):732–738.

  23. Moonmoon M, Uddin MN, Ahmed S, Shelly NJ, Khan MA (2010) Cultivation of different strains of king oyster mushroom (Pleurotus eryngii) on saw dust and rice straw in Bangladesh. Saudi J Biol Sci 17(4):341–345.

  24. Morais MH, Ramos AC, Matos N, Santos Oliveira EJ (2000) Note: production of shiitake mushroom (Lentinus edodes) on ligninocellulosic residues. Food Sci Technol Int 6:123–128.

  25. Nunes MD, Maria J, Paes SA, Júlio J, Ribeiro O (2012) Nitrogen supplementation on the productivity and the chemical composition of oyster mushroom. J Food Res 1:113–119.

  26. Obodai M, Kine JCO, Vowotor KA (2003) Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by products. J Ind Microb Biotechnol 30(3):146–149.

  27. Oseni TO, Dlamini SO, Earnshaw DM, Masarirambi MT (2012) Effect of substrate pre-treatment methods on oyster mushroom (Pleurotus ostreatus) production. Int J Agric Biol 14(2):251–255

  28. Pegler DN (1977) A preliminary agaric flora of East Africa. Kew Bull Addition Series 6, Lubrecht and Cramer Ltd., U.S. and Canada

  29. Peng JT, Dai MC, Tsai YF, Chen MH, Chen JT (2001) Selection and breeding of king oyster mushroom. J Agric Res China 50(4):43–58

  30. Periasamy K (2005) Novel antibacterial compounds obtained from some edible mushrooms. Int J Med Mushrooms 7(3):443–444.

  31. Philippoussis A, Zervakis G, Diamantopoulou P (2001) Bioconversion of agricultural lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World J Microbiol Biotechnol 17(2):191–200.

  32. Purkayastha RP, Chandra A (1985) Manual of Indian edible mushroom. Today and Tomorrow Printer and Publisher, New Delhi

  33. Rahnama N, Mamat S, Shah UKM, Ling FH, Rahman NAB, Ariff AB (2013) Effect of alkali pretreatment of rice straw on cellulase and xylanase production by local Trichoderma harzianum SNRS3 under solid state fermentation. BioResources 8(2):2881–2896

  34. Roy DA, Borthakur M, Saha AK, Joshi SR, Das P (2017) Molecular characterization and antioxidant potential of three wild culinary-medicinal mushrooms from Tripura, Northeast India. Int J Med Mushroom 19(1):55–63.

  35. Royse DJ (2014) A global perspective on the high five: Agaricus, Pleurotus, Lentinula, Auricularia and Flammulina. In: Proceedings of the 8th international conference on mushroom biology and mushroom products, New Delhi, India, pp 1–6

  36. Ruiz-Rodríguez A, Soler-Rivas C, Polonia I, Wicher HJ (2010) Effect of olive mill waste (OMW) supplementation to oyster mushrooms substrates on the cultivation parameters and fruiting bodies quality. Int Biodeterior Biodegrad 64:638–645.

  37. Shimada K, Fujikawa K, Yahara K, Nakamura T (1992) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 40(6):945–948.

  38. Shulga G, Betkers T, Shakels V, Neiberte B, Verovkins A, Brovkina J, Belous O, Ambrazaitene D, Žukauskaite A (2007) Effect of the modification of lignocellulosic materials with a lignin-polymer complex on their mulching properties. BioResources 2(4):572–582

  39. Swain T, Hillis WE (1959) The phenolic constituents of Purmus domestica. I.—the quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68.

  40. Tisdale TE, Miyasaka SC, Hemmes DE (2006) Cultivation of the oyster mushroom (Pleurotus ostreatus) on wood substrates in Hawaii. World J Microbiol Biotechnol 22:201–206.

  41. Upadhyay RC, Verma RN, Singh SK, Yadav MC (2002) Effect of organic nitrogen supplementation in Pleurotus species. In: Mushroom biology and mushroom products. The 4th ICMBP. Solan, India. UAEM. 12, pp 325–332

  42. Yang W, Guo F, Wan Z (2013) Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull. Saudi J Biol Sci 20(4):333–338.



The authors are grateful to the Head, Department of Botany, Tripura University for providing all sorts of facilities. The authors are also thankful to Forest Department of Tripura, Government of Tripura, India, for giving the permission of fieldwork in forest areas. The first author is thankful to the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India for the financial assistance received through a Project (Sanctioned Order No. BT/463/NE/TBP/2013).

Author Information

Debnath Sanjit
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India
Saha Rahul
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India

Das Panna
Microbiology Laboratory, Department of Botany, Tripura University, Agartala, India

Saha Ajay Krishna
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India