Vegetos
(An International Journal of Plant Research & Biotechnology)
Development of efficient micropropagation, assessment of genetic fidelity and biochemical fidelity in Curcuma longa L.
*Article not assigned to an issue yet
Dhondi Prasanna, Kota Srinivas, Rohela Gulab Khan, Kasula Kiranmayee
Research Articles | Published: 19 June, 2025
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Keywords: Curcumin, HPLC, In vitro regeneration, ISSR, PGR, SCoT
Abstract
Turmeric (Curcuma longa L.), a key source of curcumin, is highly demanded in food, pharmaceuticals, cosmetics, and traditional medicine industries. India, a leading producer, has benefited significantly from this demand, boosting its spice exports and ranking third globally among spice-exporting nations. To meet the growing demand and enhance turmeric production, prime cultivars like DR (DR) and BSR require efficient mass propagation methods ensuring genetic and curcumin consistency. In this context, a reliable and effective in vitro regeneration protocol was developed from unsprouted rhizome buds of these prime cultivars. On MS media with single cytokinin and auxin, dormancy breakage with highest mean shoot number of 2.40 ± 0.33 per explant was obtained in cv. DR on 0.2 mg L−1 of Kinetin (Kn) and 0.2 mg L−1 α-Naphthalene Acetic Acid (NAA) and mean shoot number of 4.90 ± 0.24 per explant was induced in cv. BSR on 0.7 mg L−1 Kn and 0.2 mg L−1 NAA. Upon sub-culturing of induced shoots on various combinations of double cytokinin with auxin supplemented media, higher frequency of shoot induction (37.30 ± 0.61 shoots in cv. DR and 26.90 ± 0.76 shoots cv. BSR), shoot elongation and rooting was achieved on 6-Benzyl Amino Purine (BAP) (2.0 mg L−1), Thidiazuron (TDZ) (2.0 mg L−1), and (NAA) (2.0 mg L−1) supplemented MS media. In vitro raised plantlets were acclimatized in field conditions with 70% survival rate. The genetic homogeneity of in vitro micropropagated plantlets were confirmed by using Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT) markers. Further, the curcumin content quantified through High Pressure Liquid Chromatography (HPLC) technique has showed biochemical fidelity of in vitro and ex vitro grown plants. In conclusion, the developed protocol proves invaluable for the micropropagation of true-to-type turmeric plants of DR and BSR cultivars.
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Author Information
Department of Biotechnology, Telangana University, Nizamabad, India