Differential biochemical response of basmati and non-basmati rice seeds upon bakanae (Fusarium fujikuroi) infection

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00411-5
First Page: 516
Last Page: 525
Views: 1425

Keywords: Bakanae, Defence, Foot rot, Peroxidase, Phenol, Resistance


Bakanae (foolish seedling) is an economically destructive seed borne fungal disease of rice prevalent all across the world. The current investigation was undertaken to decipher the effect of Fusarium fujikuroi inoculation on rice seeds by evaluating the post infection physio-biochemical changes in the basmati and non-basmati rice cultivars. Seeds of two basmati and two non-basmati rice cultivars were inoculated with the virulent isolate of F. fujikuroi, from which enzymatic antioxidants and metabolites present in seeds were quantified 48 h post inoculation. Germination studies were conducted in healthy and inoculated seeds to compare their vigour. Reduction in per cent germination, increase in root and shoot length, fresh and dry weight of seedlings in the tested cultivars post inoculation with the pathogen was observed. However, the variation in vigour traits was significant in basmati cultivars only. Total phenols and ortho-dihydroxy phenols reduced post inoculation in basmati cultivars and the reverse was seen in non- basmati cultivars. The PPO and PAL activity increased post inoculation in seeds of non-basmati rice cultivars. The present study revealed that the inherent phenolic metabolism of non-basmati rice cultivars offers resistance to disease infestation. Following responses of basmati and non-basmati rice cultivars against F. fujikuroi, it may be inferred that enzymatic activity of POD, SOD, PPO and PAL play a significant role in resistance signalling against this disease and the change in their activity could be an ideal trait for predicting resistance against bakanae.

Bakanae, Defence, Foot rot, Peroxidase, Phenol, Resistance

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Agrios GN (2005) Plant pathology, 5th edn. Academic Press, San Diego, p 200

Amatulli MT, Spadaro D, Gullino ML, Garibaldi A (2010) Molecular identification of Fusarium spp. associated with bakanae disease of rice in Italy and assessment of their pathogenicity. Pl Pathol 59:839–844

Amatulli MT, Spadaro D, Gullino ML, Garibaldi A (2012) Conventional and real-time PCR for the identification of Fusarium fujikuroi and Fusarium proliferatum from diseased rice tissues and seeds. Eur J Pl Pathol 134:401–408

Aoun M (2017) Host defense mechanisms during Fungal Pathogenesis and how these are overcome in susceptible plants: A review. Intl J Bot 13:82–102

Aragona M, Campos-Soriano L, Piombo E, Romano E, Segundo BS, Spadaro D, Infantino A (2021) Imaging the invasion of rice roots by the bakanae agent Fusarium fujikuroi using a GFP-tagged isolate. Eur J Plant Pathol 161:25–36

Bajaj KL, Arora YK, Mahajan R (1983) Biochemical diferences in tomato cultivars resistant and susceptible to Meloidogyne incognita. Revue Nematol 6:143–145

Bashyal BM (2018) Etiology of an emerging disease: bakanae of rice. Ind Phytopath 71:485–494

Bashyal BM, Aggarwal R, Banerjee S, Gupta S, Sharma S (2014) Pathogenicity, ecology and genetic diversity of the Fusarium spp. associated with an emerging bakanae disease of rice (Oryza sativa L.) in India. In: Kharwar R, Upadhyay R, Dubey N, Raghuwanshi R (eds) Microbial Diversity and Biotechnology in Food Security. Springer, New York, pp 307–314

Burrell MM, Rees TA (1974) Metabolism of phenylalanine and tyrosine in rice leaves infected by Pyricularia oryzae. Physiol Pl Pathol 4:497–474

Cheng AP, Chen SY, Lai MH, Wu DH, Lin SS, Chen CY, Chung CL (2020) Transcriptome analysis of early defenses in rice against Fusarium fujikuroi. Rice 13:65

Chhabra R, Shabnam, Singh T (2019b) Seed aging, storage, and deterioration: an irresistible physiological phenomenon. Agri Rev 40(3):234–238

Chhabra R, Kaur N, Bala A (2019a) Physiological and biochemical alterations imposed by Fusarium fujikuroi infection in aromatic and non-aromatic rice cultivars. Pl Physiol Rep 24:563–575

Chhabra R, Kaur S, Vij L, Gaur K (2020) Exploring Physiological and Biochemical Factors Governing Plant Pathogen Interaction: A Review. Int J Curr Microbiol App Sci 9(11):1650–1666

Chhabra R, Kaur N, Bala A (2022) Biochemical and anatomical characteristics of basmati and non-basmati rice (Oryza sativa L.) for resistance to foot rot. Bangladesh J Bot 51(1):29–36

Duboi M, Giles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for the determination of sugars and related substances. Anal Chem 28:350–356

Ghazanfar MU, Javed N, Wakil W, Iqbal M (2013) Screening of some fine and coarse rice varieties against bakanae disease. J Agri Res 51:41–49

Gupta AK, Singh Y, Jain AK, Singh D (2014) Prevalence and Incidence of Bakanae disease of Rice in Northern India. J Agri Search 1(4):233–237

Hossain KS, Miah MAT, Bashar MA (2013) New method for screening rice varieties against bakanae disease. Bangladesh J Bot 42:315–320

Hwang IS, Kang WR, Hwang SCB, Yun SH, Ahn IP (2013) Evaluation of Bakanae disease progression caused by Fusarium fujikuroi in Oryza sativa L. J Microbiol 51:858–865

ISTA (1999) International rules for seed testing. Seed Sci Technol 31:1–152

Kumar P, Sunder S, Singh R, Kumar A (2016) Management of foot rot and bakanae of rice through nonchemical methods. Ind Phytopath 69(1):16–20

Lee YP, Takahashi T (1966) An improved colorimetric determination of amino acids with the use of ninhydrin. Anal Biochem 14:71–75

Lee SB, Kim N, Jo S, Hur YJ, Lee JY, Cho JH, Lee JH, Kang JW, Song YC, Bombay M, Kim SR, Lee J, Seo YS, Ko JM, Park DS (2021) Mapping of a Major QTL, qBK1Z, for Bakanae Disease Resistance in Rice. Plants 10:A434

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275

Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autooxidation of pyragallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474

Matic S, Gullino ML, Davide S (2017) The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice. Front Biosci 9:333–344

Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti VB, Vandepoele K, Gollery M, Shulaev M, Van Breusegem F (2011) ROS signaling: the new wave? Trends Plant Sci 16:300–309

Murata T, Akazawa T, Furuchi S (1968) Enzymic mechanism of starch breakdown in germinating rice seeds I. An analytical study. Pl Physiol 43:1899–2005

Nelson PE, Toussou TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. Pensylvania State University, Press, London

Patel A, Patel A, Patel A, Patel NM (2010) Estimation of flavonoid, polyphenolic content and in vitro antioxidant capacity of leaves of Tephrosia purpurea Linn. (Leguminosae). Intl J Pharma Sci Res 1:66–77

Puyam A, Pannu PPS, Kaur J, Shikha S (2017) Variability in the production of gibberellic acid and fusaric acid by Fusarium moniliforme causing foot rot disease of rice and their relationship. J Pl Pathol 99:103–108

Raghu S, Guru-Pirasanna-Pandi G, Baite MS, Yadav MK, Prabhukarthikeyan SR, Keerthana U, Rath PC (2021) Estimation of yield loss and relationship of weather parameters on incidence of bakanae disease in rice varieties under shallow low land ecologies of Eastern India. J Environ Biol 42:995–1001

Ruelas C, Tiznado-Hernandez ME, Sanchez-Estrada A, Robles-Burgueno MR, Troncoso-Rojas R (2006) Changes in phenolic acid content during Alternaria alternate infection in tomato fruit. J Phytopathol 154:236–244

Saleh L, Plieth C (2009) Fingerprinting antioxidative activities in plants. Pl Methods 5:2

Sang WG, Pyeong KJH, Hyeoun SC, Myung CS, Park HK, Lee GH, Jeong NJ (2015) Physio biochemical characterisation of bakanae disease tolerant rice. J Korean Soc Int Agri 27:460–468

Shannon LM, Kay E, Lew JY (1966) Peroxidase isozymes from horse radish roots: Isolation and physical properties. J Biol Chem 241:2166–2172

Sharma S, Gupta N (2020) Defense signalling in plants against micro-creatures: do or die. Ind Phytopathol 73:605–613

Singh M, Bala R, Sharma VK, Kaur J, Sharma S (2017) Biochemical basis of resistance in wheat against Karnal bunt caused by Tilletia indica. Ind Phytopath 70:216–219

Sunani SK, Bashyal BM, Kharayat BS, Prakash G, Krishnana SG, Aggarwal R (2019) Identification of rice seed infection routes of Fusarium fujikuroi inciting bakanae disease of rice. J Pl Pathol 102:113–121

Swain T, Hills WE (1959) The phenolic constituents of Prunus domestica and the quantitative analysis of phenolic constituents. J Sci Food Agri 10:63–68

Vandana VV, Bhai RS, Azeez S (2014) Biochemical defense responses of black pepper (Piper nigrum L.) lines to Phytophthora capsici. Physiol Mol Plant Pathol 88:18–27

Vargas WA, Martin JM, Rech GE, Rivera LP, Benito EP, Diaz-Minguez JM, Thon MR, Sukno SA (2012) Plant defence mechanisms are activated during biotrophic and necrotrophic development of Colletotrichum graminicola in maize. Pl Physiol 4:457–467

Zauberman G, Ronen R, Akerman M, Weksler A, Rot I, Fuchs Y (1991) Post-harvest retention of the red colour of litchi fruit pericarp. Sci Hort 47:89–97

Zeier J (2013) New insights into the regulation of plant immunity by amino acid metabolic pathways. Plant cell and Environ 36:2085–2103

Mahadevan A, Sridhar R (1986) Methods in physiological plant pathology, 3rd Edn. Sivakami Publications, Chennai p 183–184



Author Information

Chhabra Rohit
Department of Botany, Punjab Agricultural university, Ludhiana, India
Kaur Nirmaljit
Department of Botany, Punjab Agricultural university, Ludhiana, India

Bala Anju
Department of Plant Breeding and Genetics, Punjab Agricultural university, Ludhiana, India