Vegetos

Abstract

DNA being the only basis in effecting genetic differences between distinct organisms, fingerprinting of DNA offers appropriate method of biological individualization through establishing genetic purityvariety identification in the IPR regime. RAPD primer directs amplification of several discrete loci in the genome, making the assay useful for efficient screening of nucleotide sequence polymorphism between individuals. As primers modified by a single nucleotide produce different banding profiles, RAPD technique can generate polymorphisms between very closely related genotypes. Nineteen promising cultivars of soybean grown across India in diverse agro-climatic zones having morphological diversity and varied parentage were selected for molecular polymorphism studies. In the present study, 10 RAPD primers led to amplification of 56 fragments ranging from about 3,300 bp (by OPT 1) to 500 bp (OPP 15), out of which 50 (89.29%) bands were found to be polymorphic. The level of polymorphism ranged from 50% (by OPP 19) to 100% (by OPP 6, 8, 12, 15 and OPT 13). On an average 5.6 bands per primer were obtained and all the primers produced polymorphic banding pattern indicating proper selection and usefulness of the selected primers. Soybean genotype used in the present study have got a wide genetic base and identification of genetic differences among those cultivars by DNA polymorphism could be very important for chalking out further breeding strategies.