Revathi J., Manokari M., Priyadharshini S., Shekhawat Mahipal S.
Keywords: Conservation, ex vitro rooting, Oldenlandia herbacea , plant growth regulators
Plant growth regulators (PGRs) may alter the morphogenic behavior of plant cells under in vitro conditions. Cytokinins and auxins were used to examine the response of mature nodal meristems of Oldenlandia herbacea (L.) Roxb. in this report. The 6-benzylaminopurine (BAP) alone at 1.5 mg L− 1 in Murashige and Skoog’s medium (MS) formed 5.0 ± 0.25 axillary shoots per explant but the combination of BAP, Kinetin (6-furfurylaminopurine) with indole-3-acetic acid (IAA) in the medium further proliferated the shoots exponentially (112.6 ± 0.68 shoots per explants per culture vessel). Auxins were used to induce roots from the cut ends of in vitro raised shoots via ex vitro method (in greenhouse). The highest percentage of ex vitro rooting (93%) with 11.5 ± 0.36 roots per shoot was achieved when shoots were pulse treated with 200 mg L− 1 indole-3-butyric acid (IBA) for 4 min. The regenerated plantlets were successfully acclimatized in the greenhouse and transferred to field with 89.6% survival rate. This is the foremost report on the use of plant growth regulators for effective in vitro regeneration via ex vitro rooting in O. herbacea. The developed system could be used as an alternative strategy to conserve this medicinal plant.
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Authors are grateful to the Science and Engineering Research Board, Department of Science and Technology, Government of India (sanction number EMR/2016/007795) for providing financial support to their laboratory.