Sathish Selvam, Vasudevan Venkatachalam, Karthik Sivabalan, Elayaraja Dhandapani, Pavan Gadamchetty, Ajithan Chandrasekaran, Manickavasagam Markandan
Keywords: Salicylic acid, Elicitation, Adventitious root, l-Dopa, Hybanthus , Yeast extract, Metabolites
l-Dopa is the most potent and gold standard drug for the treatment of Parkinson disease. The present finding reports the effect of elicitors on enhanced production of l-Dopa from adventitious root cultures of H. enneaspermus. Adventitious root culture was established from leaf explant of H. enneaspermus. Highest root induction frequency 70.66% with 17.76 roots per explant were recorded in explants inoculated on MS medium supplemented with Indole-3-butyric acid (IBA) 0.5 mg/l. The influence of elicitors on the biosynthesis of l-Dopa content was studied by exposure of adventitious roots to different duration and concentrations of salicylic acid (SA), yeast extract (YE), methyl jasmonate (MeJA) and silver nitrate (AgNO3). Among the different elicitors tested, exposure to SA at 100 µM dosage for 6 h was found to enhance l-Dopa yield 12.64 mg/g dry weight (DW) when compared to control culture. The present findings reveal that elicitation experiment could improve the production of l-Dopa in adventitious root cultures of H. enneaspermus and the current study may be helpful for commercial production of these essential secondary metabolites in the future for clinical applications. This is the first report on adventitious root cultures and elicitation strategy on the significant improvement in l-Dopa production from H. enneaspermus.
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The authors are thankful to University grants commission-Special Assistance Programme (UGC-SAP), New Delhi, Govt. of India for financial assistance Ref: 3–27/2011 (SAP-11) Dt/ March, 2011.