Enhanced anti-ageing and wound healing properties of Ficus religiosa L. bark, leaf and aerial root extract in human keratinocytes cell line (HaCaT)

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00094-5
First Page: 158
Last Page: 165
Views: 1985

Keywords: Anti-ageing, Astringent, Ficus religiosa L., HaCaT, MMP-1, PCOLCE, TERT, Wound healing


Plants have played a vital role in curative and preventive healthcare. Ficus religiosa L., a medicinally important tree has been used as a traditional medicine to treat various ailments and skin diseases. However, its skin anti-ageing properties are not yet scientifically evaluated. Here we report the comparison of F. religiosa bark (FRAqB), leaf (FRAqL) and aerial-roots (FRAqAR) extracts for skin anti-ageing and wound healing properties. FRAqB, FRAqL and FRAqAR extracts preparation, cell culture, RNA isolation, real time PCR, astringent activity and wound healing assay were carried out using standard methods. Axio Observer FL-microscope and ImageJ software were used for wound healing assay analysis. The real time PCR study reveals that matrix metalloproteinase-1 (MMP) RNA was downregulated to 75% and 95% for FRAqB and FRAqAR, respectively. For procollagen C-endopeptidase enhancer 1 (PCOLCE), FRAqB and FRAqL enhanced RNA levels by 500% and 300%, respectively. FRAqB and FRAqL increased wound healing area to about 60% and FRAqAR by 50%. All three extracts demonstrated astringent activity which is significant for skin tightening. The study proves that extracts of F. religiosa are prominent candidates for pharmaceutical and cosmetic applications such as skin anti-ageing and wound healing.

Anti-ageing, Astringent, Ficus religiosa L., HaCaT, MMP-1, PCOLCE, TERT, Wound healing

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This research was supported by SP1601-001 for Anti-Ageing Research Institute of BIO-FD&C Co., LTD. VA Parasharami would like to thank CSIR-NCL’s in house funding for research on F. religiosa L.

Author Information

Pandey Prem
Symbiosis Center for Nanoscience and Nanotechnology (SCNN), Symbiosis International University, Pune, India

Seo Hyo Hyun
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Kim Hye-In
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Ryu Seung Hwan
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Dingre Medini
Biochemical Research Division, GGENEX, Pune, India

Moh Sang Hyun
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Kulkarni Atul
Symbiosis Center for Nanoscience and Nanotechnology (SCNN), Symbiosis International University, Pune, India
Parasharami Varsha A.
Plant Tissue Culture Division, National Chemical Laboratory, Pune, India