Pandey Prem, Seo Hyo Hyun, Kim Hye-In, Ryu Seung Hwan, Dingre Medini, Moh Sang Hyun, Kulkarni Atul, Parasharami Varsha A.
Keywords: Anti-ageing, Astringent, Ficus religiosa L., HaCaT, MMP-1, PCOLCE, TERT, Wound healing
Plants have played a vital role in curative and preventive healthcare. Ficus religiosa L., a medicinally important tree has been used as a traditional medicine to treat various ailments and skin diseases. However, its skin anti-ageing properties are not yet scientifically evaluated. Here we report the comparison of F. religiosa bark (FRAqB), leaf (FRAqL) and aerial-roots (FRAqAR) extracts for skin anti-ageing and wound healing properties. FRAqB, FRAqL and FRAqAR extracts preparation, cell culture, RNA isolation, real time PCR, astringent activity and wound healing assay were carried out using standard methods. Axio Observer FL-microscope and ImageJ software were used for wound healing assay analysis. The real time PCR study reveals that matrix metalloproteinase-1 (MMP) RNA was downregulated to 75% and 95% for FRAqB and FRAqAR, respectively. For procollagen C-endopeptidase enhancer 1 (PCOLCE), FRAqB and FRAqL enhanced RNA levels by 500% and 300%, respectively. FRAqB and FRAqL increased wound healing area to about 60% and FRAqAR by 50%. All three extracts demonstrated astringent activity which is significant for skin tightening. The study proves that extracts of F. religiosa are prominent candidates for pharmaceutical and cosmetic applications such as skin anti-ageing and wound healing.
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This research was supported by SP1601-001 for Anti-Ageing Research Institute of BIO-FD&C Co., LTD. VA Parasharami would like to thank CSIR-NCL’s in house funding for research on F. religiosa L.