Enhancement in production of baicalein through transformation in Oroxylum indicum (L.) Vent by Rhizobium rhizogenes

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00602-8
First Page: 305
Last Page: 320
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Keywords: n Rhizobiumn rhizogenesn , Hairy roots, n Oroxylumn indicumn , Flavonoid, UV–VIS spectroscopy, FTIR


Abstract


Oroxylum indicum (L) Vent (O. indicum), a significant endangered medicinal plant, was transformed by the Rhizobium rhizogenes (R. rhizogenes) mediated transformation method using the bacterial strain MTCC 532. For a successful transformation, factors such as explant selection, Co-cultivation time, temperature for induced root development, transformation technique, and antibiotic concentration were optimized. Various methods were employed for enhancing the rate of transformation. A drastic increase in transformation frequency was observed when CaCl2 was used in concentration of 10 mM and 15 mM along with ultrasonication during Co-cultivation. Total phenolic and flavonoid content was determined in various extracts of O. indicum by using Folin–Ciocalteau reagent and Aluminium chloride colorimetric method respectively. Moreover, the antioxidant potential of different extracts of O. indicum were assessed with the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. Using specific rolA primers, molecular analysis was performed, revealing T-DNA integration in the hairy roots and confirming the expression of hairy root inducible genes. Murashige and Skoog (MS) medium with 3% sucrose was shown to have the maximum induction rate of hairy roots after 28 days of Co-cultivation. TLC as well as spectroscopic methods (UV–VIS and FTIR) were performed to compare the specific flavonoid (baicalein) in transformed roots as well as several non-transformed extracts. Comparing hairy roots to non-transformed roots, the total phenolic, flavonoid contents, and antioxidant activities were greater in hairy roots. The findings indicate that O. indicum hairy root cultures have a greater capacity for producing beneficial chemicals and researching their biological activities. The transformation of O. indicum by R. rhizogenes (MTCC 532) has been reported for the first time.


n              Rhizobiumn              rhizogenesn            , Hairy roots, n              Oroxylumn              indicumn            , Flavonoid, UV–VIS spectroscopy, FTIR


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Acknowledgements


We are grateful to the Principal, St. Aloysius(autonomous) College, Jabalpur (MP, India) for supporting this research.


Author Information


Faraz Rumana
Department of Biotechnology, St. Aloysius (Autonomous) College, Jabalpur, India
rfaraz82@gmail.com
Gokhale Mamta
Department of Botany and Microbiology, St. Aloysius (Autonomous) College, Jabalpur, India


Gothalwal Ragini
Department of Biotechnology, Barkatullah University, Bhopal, India