Enhancing germination and early seedling growth and tolerance of barley under Cu stress using gibberellic acid

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Research Articles | Published:

E-ISSN: 2229-4473.
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DOI: 10.1007/s42535-025-01500-x
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Keywords: Copper, Seeds germination, Barley seeds, Tolerance index, Gibberellic acid


Abstract


Soil pollution with metals seriously threatens the ecosystem and destroys the environment. Copper (Cu), as an essential micronutrient, is beneficial to plants at low doses and may cause significant damage when it exceeds tolerable doses. The present experiment was conducted to evaluate the role of gibberellic acid at 100, 300 and 500 mg L−1 in mitigating the negative effects of Cu (100 mg L−1 and 200 mg L−1) on germination and early seedling growth of barley species. While Cu concentrations had no effect on germination, they significantly reduced root and shoot elongation, tolerance index (TI), and seed vigor index (SVI), and increased phytotoxicity. The combination of Cu and GA increased root and shoot length by up to 47%, TI by 14.2%, SVI by 135%, and reduced phytotoxicity by 16% compared to Cu200 treatment, indicating that gibberellic acid plays a crucial role in alleviating the negative effects of Cu on barley seedlings.

Copper, Seeds germination, Barley seeds, Tolerance index, Gibberellic acid


References


Anwar T, Qureshi H, Jabeen M et al (2024a) Mitigation of cadmium-induced stress in maize via synergistic application of biochar and gibberellic acid to enhance morpho-physiological and biochemical traits. BMC Plant Biol 24:1–16. https://doi.org/10.1186/s12870-024-04805-2


Anwar T, Qureshi H, Siddiqi EH, Ullah N, Naseem MT, Soufan W (2024b) Synergistic effects of gibberellic acid, biochar, and rhizobacteria on wheat growth under heavy metal and drought stress. BMC Plant Biol. https://doi.org/10.1186/s12870-024-05833-8


Baruah N, Mondal SC, Farooq M, Gogoi N (2019) Influence of heavy metals on seed germination and seedling growth of wheat, pea, and tomato. Water Air Soil Pollut. https://doi.org/10.1007/s11270-019-4329-0


Bekele MN, Gerima AH, Derartu HK, E MG, (2016) Seed germination and seedling growth of haricot bean (Phaseolus vulgaris L) cultivars as influenced by copper sulphate. African J Plant Breed 3:156–160


Ben Massoud M, Karmous I, El Ferjani E, Chaoui A (2018) Alleviation of copper toxicity in germinating pea seeds by IAA, GA3, Ca and citric acid. Journal of Plant Interactions 13(1):21–29. https://doi.org/10.1080/17429145.2017.1410733


Bhat JA, Basit F, Alyemeni MN et al (2023) Gibberellic acid mitigates nickel stress in soybean by cell wall fixation and regulating oxidative stress metabolism and glyoxalase system. Plant Physiol Biochem 198:107678. https://doi.org/10.1016/j.plaphy.2023.107678


De Almeida AAF, Valle RR, Mielke MS, Gomes FP (2007) Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr. Braz J Plant Physiol 19:83–98. https://doi.org/10.1590/S1677-04202007000200001


El Rasafi T, Bouda S, Nouri M, Haddioui A (2020) Assessment of metals (Cu, Ni) and metalloids (As) induced stress responses in Barley (Hordeum vulgare) and wheat (Triticum aestivum). J Mater Environ Sci 2020:795–807


El Rasafi T, Bouda S, Hamdali H, Haddioui A (2021) Seed germination and early seedling growth of fenugreek (Trigonella foenum-gracium L.) under Cu, Ni and As stress. Acta Ecol Sin 41:223–227. https://doi.org/10.1016/j.chnaes.2021.02.014


El Rasafi T, Haouas A, Tallou A et al (2023) Recent progress on emerging technologies for trace elements-contaminated soil remediation. Chemosphere 341:140121. https://doi.org/10.1016/j.chemosphere.2023.140121


El Rasafi T, Oukkaroum A, Haddioui A (2025) Improvement of root architecture, Pb, Cu and Zn uptake, translocation and phytoremediation efficiency of barley (Hordeum vulgare) through the application of biochar and sheep manure. Int J Phytoremediation. https://doi.org/10.1080/15226514.2025.2498672


Elleuch A, Chaâbene Z, Grubb DC et al (2013) Morphological and biochemical behavior of fenugreek (Trigonella foenum-graecum) under copper stress. Ecotoxicol Environ Saf 98:46–53. https://doi.org/10.1016/j.ecoenv.2013.09.028


Feng NX, Yu J, Zhao HM et al (2017) Efficient phytoremediation of organic contaminants in soils using plant–endophyte partnerships. Sci Total Environ 583:352–368. https://doi.org/10.1016/j.scitotenv.2017.01.075


Gong Q, Li Z, Wang L, Zhou J, Kang Q, Niu D (2021) Gibberellic acid application on biomass, oxidative stress response, and photosynthesis in spinach (Spinacia oleracea L.) seedlings under copper stress. Environ Sci Pollut Res 28(38):53594–53604. https://doi.org/10.1007/s11356-021-13745-5


Huang Y, Cai S, Ye L et al (2016) The effects of GA and ABA treatments on metabolite profile of germinating barley. Food Chem 192:928–933. https://doi.org/10.1016/j.foodchem.2015.07.090


Inayat H, Saif H, Danish S et al (2024) Improving growth of Solanum melongena L. exposed to lead (Pb) stress using gibberellic acid in combination with Agrobacterium fabrum. Plant Stress 13:100503. https://doi.org/10.1016/j.stress.2024.100503


Javed T, Ali MM, Shabbir R et al (2021) Alleviation of copper-induced stress in pea (Pisum sativum l.) through foliar application of gibberellic acid. Biology (Basel) 10:1–14. https://doi.org/10.3390/biology10020120


Kalai T, Khamassi K, Teixeira da Silva JA et al (2013) Cadmium and copper stress affect seedling growth and enzymatic activities in germinating barley seeds. Arch Agron Soil Sci 60:765–783. https://doi.org/10.1080/03650340.2013.838001


Kamboj A, Sangha MK, Devi V et al (2024) Enhancing germination and growth in wild okra genotypes through gibberellic acid priming. Sci Hortic (Amsterdam) 334:113332. https://doi.org/10.1016/j.scienta.2024.113332


Moreira IN, Martins LL, Mourato MP (2020) Effect of Cd, Cr, Cu, Mn, Ni, Pb and Zn on seed germination and seedling growth of two lettuce cultivars (Lactuca sativa L.). Plant Physiol Rep 25:347–358. https://doi.org/10.1007/s40502-020-00509-5


El Moukhtari A, El Rasafi T, Lamsaadi N, et al (2024) Bioremediation of heavy metals: Promising strategies for enhancing crop growth and productivity. In: Ditta A, Mehmood S, Imtiaz M, Tu MS (eds) Bio-organic Amendments for Heavy Metal Remediation. Elsevier Inc, pp 515–531


Munzuroglu O, Zengin FK (2006) Effect of cadmium on germination, coleoptile and root growth of barley seeds in the presence of gibberellic acid and kinetin. J Environ Biol 27:671–677


Nouri M, Haddioui A (2021) Improving seed germination and seedling growth of Lepidium sativum with different priming methods under arsenic stress. Acta Ecol Sin 41:64–71. https://doi.org/10.1016/j.chnaes.2020.12.005


Ogugua UV, Kanu SA, Ntushelo K (2022) Gibberellic acid improves growth and reduces heavy metal accumulation: a case study in tomato (Solanum lycopersicum L.) seedlings exposed to acid mine water. Heliyon 8:e12399. https://doi.org/10.1016/j.heliyon.2022.e12399


Patra DK, Pradhan C, Patra HK (2020) Toxic metal decontamination by phytoremediation approach: concept, challenges, opportunities and future perspectives. Environ Technol Innov 18:100672. https://doi.org/10.1016/j.eti.2020.100672


Qayyum S, Majid S, Bibi A, Ulfat A, Khanum K, Munir A, Nisar S, Aziz S, Mumtaz N (2018) Effect of seed priming with hormonal combinations on morphological and biochemical attributes of maize seedlings. Phyton-Int J Experim Botany 87:191–197. https://doi.org/10.32604/phyton.2018.87.191


Rani S, Kumar P, Dahiya P, Gupta A, Arora K, Dang AS, Suneja P (2024) Effect of biopriming and nanopriming on physio-biochemical characteristics of Cicer arietinum L. under drought stress. Plant Stress. https://doi.org/10.1016/j.stress.2024.100466


Saleem MH, Kamran M, Zhou Y et al (2020) Appraising growth, oxidative stress and copper phytoextraction potential of flax (Linum usitatissimum L.) grown in soil differentially spiked with copper. J Environ Manage 257:109994. https://doi.org/10.1016/j.jenvman.2019.109994


Shahzad K, Hussain S, Arfan M et al (2021) Exogenously applied gibberellic acid enhances growth and salinity stress tolerance of maize through modulating the morpho-physiological, biochemical and molecular attributes. Biomolecules 11:1–17. https://doi.org/10.3390/biom11071005


Shoaib S, Iqbal RK, Ashraf H, Younis U, Rasool MA, Ansari MJ, Alarfaj AA, Alharbi SA (2025) Mitigating effect of γ-aminobutyric acid and gibberellic acid on tomato plant cultivated in Pb-polluted soil. Sci Rep 15(1):1–13. https://doi.org/10.1038/s41598-025-96450-4


Singh D, Nath K, Sharma YK (2007) Response of wheat seed germination and seedling growth under copper stress. J Environ Biol 28:409–414


Thakur M, Tiwari S, Kataria S, Anand A (2022) Recent advances in seed priming strategies for enhancing planting value of vegetable seeds. Sci Hortic. https://doi.org/10.1016/j.scienta.2022.111355


Wunnoo S, Maneerat T, Lerslerwong L et al (2024) Effective techniques to break seed dormancy in Rhodomyrtus tomentosa (Aiton) Hassk. for seed germination enhancement. J Appl Res Med Aromat Plants 42:100558. https://doi.org/10.1016/j.jarmap.2024.100558


Zhao X, Chul J, Young J (2021) Evaluation of heavy metal phytotoxicity to Helianthus annuus L using seedling vigor index-soil model. Chemosphere 275:130026. https://doi.org/10.1016/j.chemosphere.2021.130026

 


Author Information


Health, Environment and Biotechnology Laboratory, Faculty of Sciences Ain Chock, Hassan II University, Casablanca, Maarif, Casablanca, Morocco