Keywords: Waste valorization, Poly-3-hydroxybutyrate (PHB), Synechococcus elongatus , Sustainable development, Biorefinery
Waste valorization is a technology aimed towards the conversion of waste materials into commercially applicable products like chemicals, materials, and fuels. This technology has revolutionized the field of waste management. The ‘biorefinery’ concept based on biomass-based production techniques is pitched to replace oil-based production technologies. The current research study combines these principles and proposes an organic industrial waste valorization model by biosynthesis of poly-3-hydroxybutyrate (PHB) in cyanobacteria Synechococcus elongatus. Dairy, sugar industry, fruit industry and biodiesel industry waste are used in this study. Our findings show that sugar industry waste is the most productive waste media for PHB synthesis and it yielded 9.84% of the biopolymer. The PHA inclusions are visualized by fluorescent microscopy and the chemical characteristics are studied by FTIR and GC–MS analysis. FTIR spectra reveal five peaks at 1178 cm−1, 1280 cm−1, 1379 cm−1, 1718 cm−1 and 2873 cm−1 corresponding to the characteristic chemical groups of PHB. GC–MS spectra show the peak of methyl ester derivative of 3-hydroxybutyric acid at Rt 2.6 min based on the NIST reference library. It elucidates that the PHB yield is influenced by C:N ratio of the waste sample which provides insights on making the bioplastics production process productive. The current study is significant as it projects an apt substitute for synthetic plastics at a low cost and showcases technology for the management of organically rich industrial waste. The development of such models will lead to environmental improvement and help in achieving the goals of sustainable development.
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Department of Botany, Institute of Science, Mumbai, India