Enzyme inhibitors from endophytic Xylaria feejeensis isolated from Cardiospermum halicacabum

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Research Articles | Published:

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DOI: 10.1007/s42535-025-01495-5
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Keywords: n Cardiospermum halicacabumn , Endophytic fungi, Secondary metabolites, n Xylaria feejeensisn


Abstract


An endophytic fungus Xylaria feejeensis was isolated from the leaves of Cardiospermum halicacabum and identified using molecular means. The fungus was cultured on a large scale in potato dextrose broth medium. After 4 weeks, the fungal media and mycelium were extracted with ethyl acetate and subjected to chromatographic separation furnished eight compounds namely, (R)-(-)-5-methoxycarbonylmellein (1), (S)-(+)-mellein (2), (S)-(+)-O-methylmellein (3), cycloepoxytriol A (4), 2-hydroxy-5-methoxy-3-methylcyclohexa-2,5-diene-1,4-dione (5), 2-chloro-5-methoxy-3-methylcyclohexa-2,5-diene-1,4-dione (6), phomopsiketone B (7) and 6-(1-hydroxypentyl)-4-methoxy-2H-pyran-2-one (8). Compounds 4 and 6 were isolated from the endophytic fungus X. feejeensis for the first time. Compound 1 exhibited α-glucosidase inhibitory activity with an IC50 value of 46.54 ± 8.08 µM, which was significantly lower than that of the positive control acarbose.

n                     Cardiospermum halicacabumn                  , Endophytic fungi, Secondary metabolites, n                     Xylaria feejeensisn


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Author Information


National Institute of Fundamental Studies, Kandy, Sri Lanka