Vegetos
(An International Journal of Plant Research & Biotechnology)
Estimation of genetic diversity for interspecific hybridization in Pleurotus spp
Barh Anupam, Sharma V. P., Annepu Sudheer Kumar, Kumari Babita, Kamal Shwet, Kumar Anil
Research Articles | Published: 29 January, 2023
First Page: 155
Last Page: 164
Views: 2680
Keywords: Oyster mushroom, ITS, ISSR, Genetic improvement, Mahalanobis distance, Transgressive segregants
Abstract
Pleurotus spp. is a taxonomically diverse group of species having a large range of distribution. The trait enhancement for commercial exploitation can be possible using inter and intraspecific hybridization in consideration of mating behaviour. In our study, we used forty-eight strains of the Pleurotus spp. to select the parents for interspecific hybridization. Diversity was selected as the criteria for selection and was estimated at the molecular and phenotypic levels. Nine inter-simple sequence repeat (ISSR) markers were used for the estimation of molecular diversity. All 48 strains were segregated into four species based on ITS maker (internal transcribed spacer) molecular identification. ISSR marker-based Unweighted Pair-Group Method with Arithmetic Mean (UPGMA) dendrogram was created, and diverse strains were identified. The phenotypic genetic divergence based on quantitative characters was also computed using Mahalanobis D2 statistics. Based on the genetic distance from polymorphism information content, mahalanobis distance, and mean of biological efficiency, the parental combinations of DMRP-10 × DMRP-45, DMRP-25 × DMRP-36, DMRP-8 × DMRP-25, DMRP-25 × DMRP-41, and DMRP-10 × DMRP-25 were identified for further hybridization.
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Author Information
ICAR-Directorate of Mushroom Research, Solan, India