Estimation of genetic diversity for interspecific hybridization in Pleurotus spp

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00575-8
First Page: 155
Last Page: 164
Views: 1157


Keywords: Oyster mushroom, ITS, ISSR, Genetic improvement, Mahalanobis distance, Transgressive segregants


Abstract


Pleurotus spp. is a taxonomically diverse group of species having a large range of distribution. The trait enhancement for commercial exploitation can be possible using inter and intraspecific hybridization in consideration of mating behaviour. In our study, we used forty-eight strains of the Pleurotus spp. to select the parents for interspecific hybridization. Diversity was selected as the criteria for selection and was estimated at the molecular and phenotypic levels. Nine inter-simple sequence repeat (ISSR) markers were used for the estimation of molecular diversity. All 48 strains were segregated into four species based on ITS maker (internal transcribed spacer) molecular identification. ISSR marker-based Unweighted Pair-Group Method with Arithmetic Mean (UPGMA) dendrogram was created, and diverse strains were identified. The phenotypic genetic divergence based on quantitative characters was also computed using Mahalanobis D2 statistics. Based on the genetic distance from polymorphism information content, mahalanobis distance, and mean of biological efficiency, the parental combinations of DMRP-10 × DMRP-45, DMRP-25 × DMRP-36, DMRP-8 × DMRP-25, DMRP-25 × DMRP-41, and DMRP-10 × DMRP-25 were identified for further hybridization.


Oyster mushroom, ITS, ISSR, Genetic improvement, Mahalanobis distance, Transgressive segregants


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References


Albani MC, Battey NH, Wilkinson MJ (2004) The development of ISSR-derived SCAR markers around the Seasonal flowering locus (SFL) in Fragaria vesca. Theor Appl Genet 109:571–579. https://doi.org/10.1007/s00122-004-1654-4


Cohen L, Persky Y, Hadar R, Persky L, Hadar Y (2002) Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol 58:582–594. https://doi.org/10.1007/s00253-002-0930-y


Das S, Deb B (2015) DNA barcoding of fungi using ribosomal ITS marker for genetic diversity analysis: a review. Int J Pure Appl Biosci 3:160–167


Gonzalez P, Labarère J (2000) Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains. Microbiology 146:209–221. https://doi.org/10.1099/00221287-146-1-209


Guzman G (2000) Genus Pleurotus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae): diversity, taxonomic problems, and cultural and traditional medicinal uses. Int J Med Mushrooms 2:29. https://doi.org/10.1615/IntJMedMushr.v2.i2.10


He L, Yao K, Xu X et al (2022) Intraspecific identification of lingzhi medicinal mushroom, Ganoderma lingzhi (Agaricomycetes), using ISSR markers. Int J Med Mushrooms 24:43–52. https://doi.org/10.1615/INTJMEDMUSHROOMS.2022043141


Joshi M, Verma SK, Singh JP, Barh A (2013) Genetic diversity assessment in Lentil (Lens culinaris Medikus) genotypes through ISSR marker. The Bioscan 8:1529–1532


Lallawmsanga PAK, Mishra VK et al (2016) Antimicrobial potential, identification and phylogenetic affiliation of wild mushrooms from two sub-tropical semi-evergreen indian forest ecosystems. PLoS ONE 11:e0166368. https://doi.org/10.1371/journal.pone.0166368


Liu J, Wang Z-R, Li C et al (2015) Evaluating genetic diversity and constructing core collections of Chinese Lentinula edodes cultivars using ISSR and SRAP markers. J Basic Microbiol 55:749–760. https://doi.org/10.1002/jobm.201400774


Menolli Junior N, Asai T, Capelari M, Paccola-Meirelles LD (2010) Morphological and molecular identification of four Brazilian commercial isolates of Pleurotus spp. and cultivation on corncob. Brazilian Arch Biol Technol 53:397–408. https://doi.org/10.1590/S1516-89132010000200019


Nazrul MI, YinBing B (2011) Differentiation of homokaryons and heterokaryons of Agaricus bisporus with inter-simple sequence repeat markers. Microbiol Res 166:226–236. https://doi.org/10.1016/J.MICRES.2010.03.001


Rao CR (1952) Advance statistical methods in biometrical research. John Wiley and Sons Inc., New York


Rohlf FJ (2008) NTSYSpc: numerical taxonomy system, ver. 2.20.


Royse BJ, Tan Q (2017) Current overview of mushroom production in the world. In: Zied DC, Giménez, Arturo P (eds) Edible and medicinal mushrooms: technology and applications, 1st edn. John Wiley & Sons Ltd, New York, pp 5–13


Shnyreva AA, Shnyreva AV (2015) Phylogenetic analysis of Pleurotus species. Genetika 51:177–187


Su H, Wang L, Liu L et al (2008) Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes. J Appl Genet 49:233–235. https://doi.org/10.1007/BF03195619


Sudhir K, Glen S, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874


Tang L, Xiao Y, Li L et al (2010) Analysis of genetic diversity among Chinese auricularia auricula cultivars using combined ISSR and SRAP markers. Curr Microbiol 61:132–140. https://doi.org/10.1007/s00284-010-9587-4


Upadhyay R, Verma B, Sood S et al (2017) Documentary of agaricomycetous mushrooms of India orders—agaricales, boletales and russulales. JayaPublishing House, Delhi


Zhao M, Huang C, Chen Q et al (2013) Genetic variability and population structure of the mushroom Pleurotus eryngii var. tuoliensis. PLoS ONE 8:e83253. https://doi.org/10.1371/journal.pone.0083253




 


Acknowledgements


Authors would like to acknowledge the support of Indian Council for Agricultural Research for conducting the research.


Author Information


Barh Anupam
ICAR-Directorate of Mushroom Research, Solan, India
anupambarh6@gmail.com
Sharma V. P.
ICAR-Directorate of Mushroom Research, Solan, India


Annepu Sudheer Kumar
ICAR-Indian Institute of Soil and Water Conservation, Udhagamandalam, India


Kumari Babita
ICAR-Directorate of Mushroom Research, Solan, India


Kamal Shwet
ICAR-Directorate of Mushroom Research, Solan, India

Kumar Anil
ICAR-Directorate of Mushroom Research, Solan, India