Evaluation of divergence in underutilized wonder legume winged bean [Psophocarpus tetragonolobus (L.) DC.] using ISSR markers

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00282-2
First Page: 140
Last Page: 148
Views: 1818

Keywords: Underutilized legume, Genetic variation, Molecular markers


Winged bean (Psophocarpus tetragonolobus (L.) DC) is a nutritious tropical legume known for its high protein content in the seeds. Twenty winged bean genotypes were studied for the genetic diversity using Inter-simple sequence repeat (ISSR) markers. On ISSR analysis, 124 bands were generated by 11 informative primers, of which 76 bands were polymorphic and 3 were unique. The PIC values of ISSR primers studied ranged from 0.06 to 0.39; resolving power (Rp) values ranged from 0.9 to 4.7 and the mean number of bands per locus ranged from 9.6 to 18.2. The mean number of bands per accession ranged from 3.9 to 13.7. Narrow genetic base was revealed with the similarity values amongst genotypes ranging from 0.72 to 0.98; with 64 combinations having similarity value more than 0.90 and another 105 genotypic combinations having similarity index in between 0.80 and 0.90. The dendrogram showed two major clusters with 4 groups; with cluster-I being mono-genotypic (EC142666); while two little bit divergent groups could be clearly distinguished from highly homogenous core twelve genotypes. The principal component analysis (PCA) derived pattern was almost in accordance with the clustering pattern based dendrogram. Within the narrow base of the winged bean genotypes studied; four main groups were observed with EC142666 being most divergent; followed by two diverse groups comprising of 3 (EC-178268/EC-178269/EC-178311) and 4 (EC-121921/EC-178291/EC-178310/EC-251020) genotypes respectively; with the other genotypes were very close to each other indicating genetic bottleneck. This necessitates the need for identification of divergence germplasm for breeding to broaden the genetic base.

Underutilized legume, Genetic variation, Molecular markers

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Alikhani L, Rahmani MS, Shabanian N, Badakhshan H, Khadivi-Khub A (2014) Genetic variability and structure of Quercus brantii assessed by ISSR, IRAP and SCoT markers. Gene 552:176–183

Aljanabi S, Forget L, Dookun A (1999) An improved and rapid protocol for the isolation of polysaccharide-and polyphenol-free sugarcane DNA. Plant Mol Biol Report 17:1–8

Charu D, Kharb P, Sharma R, Uppal S, Aggarwal RK (2013) Development of male-specific SCAR marker in date palm (Phoenix dactylifera L.). Tree Genet Genomes 9(5):1143–1150

Chen D, Yi X, Yang H, Zhou H, Yu Y, Tian Y, Lu X (2015) Genetic diversity evaluation of winged bean (Psophocarpus tetragonolobus (L.) DC.) using inter-simple sequence repeat (ISSR). Genet Resour Crop Evol 62:823–828

Darvasi A, Soller M (1994) Optimum spacing of genetic markers for determining linkage between marker loci and quantitative trait loci. Theor Appl Genet 89:351–357

De Riek J, Calsyn E, Everaert I, Van Bockstaele E, De Loose M (2001) AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. Theor Appl Genet 103:1254–1265

Foyer CH, Lam HM, Nguyen HT, Siddique KH, Varshney RK, Colmer TD, Cowling W, Bramley H, Mori TA, Hodgson JM, Cooper JW (2016) Neglecting legumes has compromised human health and sustainable food production. Nat Plants 2(8):1–10

Gonçalves LO, Pinheiro JB, Zucchi MI, Silva-Mann R (2014) Genetic characterization of the coral tree (Erythrina velutina Willd.) in areas of low occurrence. Rev Cienc Agron 45:290–298

Kortt AA (1980) Isolation and properties of a chymotrypsin inhibitor from winged Bean seed (Psophocarpus tetragonolobus (L.) DC.). Biochim Biophys Acta 624:237–248

Koshy EP, Alex BK, John P (2013) Clonal fidelity studies on regenerants of Psophocarpus tetragonolobus (L.) DC. using RAPD markers. The Bioscan 8:763–766

Lazaroff L (1989) Strategy for development of a new crop. In: Wickens GE, Haq N, Day P (eds) New crops for food and industry. Springer, London, pp 108–119

Marotti I, Bonetti A, Minelli M, Catizone P, Dinelli G (2007) Characterization of some Italian common bean (Phaseolus vulgaris L.) landraces by RAPD, semi-random and ISSR molecular markers. Genet Resour Crop Evol 54:175–188

Mohanty CS, Verma S, Singh V, Khan S, Gaur P, Gupta P, Nizar MA, Dikshit N, Pattanayak R, Shukla A, Niranjan A, Sahu N, Behera SK, Rana TS (2013) Characterization of winged bean (Psophocarpus tetragonolobus (L.) DC.) based on molecular, chemical and physiological parameters. Am J Mol Biol 3:187–197

Mohanty CS, Singh V, Gupta P, Srivsatava A, Pattanayak R, Jaiswal V, Dikshit N (2019) Estimation of genetic diversity, structure and trait association of winged bean (Psophocarpus tetragonolobus (L.) DC.), genotypes through AFLP and ITS markers. Indian J Biotechnol 18:235–245

Mokate YS, Chimote VP, Deshmukh MP, Gawai K, Kale AA, Jadhav AS (2017) Use of promoter-anchored RAPD analysis for divergence assessment in soybean as against conventionally used ISSR assay. Appl Biol Res 19:1–9

Nei M, Li W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

Paterson AH, Tanksley SD, Sorrells ME (1991) DNA markers in plant improvement. Adv Agron 46:39–40

Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–112

Reddy MP, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:7–17

Rohlf FJ (2004) NTSYSpc Numerical taxonomy and multivariate analysis system Version 2.1.

Sharma S, Upadhyaya HD, Varshney RK, Gowda CL (2013) Pre-breeding for diversification of primary gene pool and genetic enhancement of grain legumes. Front Plant Sci 4:309

Sneath PHA, Sokal RR (1973) Numerical taxonomy: The principles and practices of numerical classification (Freeman, San Francisco), pp 1–573.

Velasco-Ramírez AP, Torres-Morán MI, Molina-Moret S, Sánchez-González JD, Santacruz-Ruvalcaba F (2014) Efficiency of RAPD, ISSR, AFLP and ISTR markers for the detection of polymorphisms and genetic relationships in camote de cerro (Dioscorea spp.). Electron J Biotechnol 17:65–71

Wong QN, Tanzi AS, Ho WK, Malla S, Blythe M, Karunaratne A, Massawe F, Mayes S (2017) Development of gene-based SSR markers in winged bean (Psophocarpus tetragonolobus (L.) DC.) for diversity assessment. Genes 8(3):100

Yanagi SO (1984) Winged bean protein in comparison to other legume proteins. Jpn Agric Res Q 18:53–59

Yang S, Grall A, Chapman MA (2018) Origin and diversification of winged bean (Psophocarpus tetragonolobus (L.) DC.), a multipurpose underutilized legume. Am J Bot 105(5):888–897

Zhang K, Wu Z, Li Y, Zhang H, Wang L, Zhou Q, Tang D, Fu Y, He F, Jiang Y, Yang H, Wang J (2014) ISSR-Based molecular characterization of an elite germplasm collection of sweet potato (Ipomoea batatas L.) in China. J Integr Agric 13:2346–2361

Ojuederie OB, Nkang NA, Odesola KA, Igwe DO (2020) Genetic diversity assessment of winged bean (Psophorcarpus tetragonolobus) accessions revealed by Inter-Simple Sequence Repeat (ISSR) markers. J Plant Biol Crop Res 3(1):1014



Authors are thankful to authorities of Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, India for providing necessary facilities to undertake this work. Help provided by our colleagues during this study is also acknowledged.

Author Information

Dieu Tran Quang
Department of Agricultural Botany, Mahatma Phule Krishi Vidyapeeth, District Ahmednagar, India