Field application of functionalized carbon nanotubes improves wheat yield without residue translocation

*Article not assigned to an issue yet


Research Articles | Published:

E-ISSN: 2229-4473.
Website: www.vegetosindia.org
Pub Email: contact@vegetosindia.org
DOI: 10.1007/s42535-026-01745-0
First Page: 0
Last Page: 0
Views: 2

Keywords: Functionalised multiwalled carbon nanotubes, n Triticum aestivum L., Seed germination, Vegetative growth parameters


Abstract


This study examines the influence of functionalised multiwalled carbon nanotubes (MWCNTs) on the germination, vegetative growth, and yield performance of Triticum aestivum L. (wheat). MWCNTs were synthesised via chemical vapour deposition and functionalised through acid treatment to improve dispersibility and biocompatibility. Comprehensive characterisation using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM) confirmed successful surface modification. Field trials were conducted using a randomised block design with foliar application of functionalised MWCNTs at concentrations of 0, 20, 30, 40, and 50 µg mL− 1 on the wheat cultivar HD 2967. Results indicated a concentration-dependent enhancement across all measured parameters, including germination rate, plant height, tiller number, spike length, and yield components such as grain number per spike and 1000-grain weight. The T4 treatment (50 µg mL− 1) exhibited the greatest improvements, with grain and straw yields increasing by 24% and 31%, respectively, over the control. Raman spectroscopic analysis revealed no detectable MWCNT residues in harvested grains, suggesting no translocation to edible tissues and supporting biosafety at the applied dosages. These findings underscore the potential of functionalised MWCNTs as nano-enabled biostimulants for sustainable wheat production.

Functionalised multiwalled carbon nanotubes, n                     Triticum aestivum L., Seed germination, Vegetative growth parameters


References


Arain ZA, Ali U, Bibi S, Khaskheli MA, Pehlwan B, Bhutto A, Bhutto MK, Rajput JA, Bhan RA, Sipio WD, Ullah R, Siddiqui A (2024) The foliar application of nitrogen and zinc applied during tillering and booting stage enhanced the growth and production of wheat (Triticum Aestivum L). Pak J Biotechnol 21(1):178–183. https://doi.org/10.34016/pjbt.2024.21.01.897


Bárzana G, Garcia-Gomez P, Carvajal M (2022) Nanomaterials in plant systems: Smart advances related to water uptake and transport involving aquaporins. Plant nano biology 1:100005. https://doi.org/10.1016/j.plana.2022.100005


Bibi A, Khan RS, Iqbal A, Ullah A, Khan MA, Law D, Iqbal MZ, Ahmad A (2026) Harnessing carbon nanotubes in Moringa oleifera micropropagation for enhanced morphological traits, phytochemicals, and phytohormones production. J Hortic Sci Biotechnol 1–13. https://doi.org/10.1080/14620316.2025.2611792


Chen J, Zeng X, Yang W, Xie H, Ashraf U, Mo Z, Liu J, Li G, Li W (2021) Seed Priming with Multiwall Carbon Nanotubes (MWCNTs) Modulates Seed Germination and Early Growth of Maize Under Cadmium (Cd) Toxicity. J Soil Sci Plant Nutr 21:1793–1805. https://doi.org/10.1007/s42729-021-00480-6


Gautam SS (2019) PhD thesis, Dayalbagh Educational Institute, Agra Uttar Pradesh, India. http://hdl.handle.net/10603/312897


Gautam SS, Satsangi GP, Satsangi VR (2020) Chemical synthesis, functionalization and characterization of multiwalled carbon nanotubes. J Nanosci Technol 6(3):905–907. https://doi.org/10.30799/jnst.307.20060302


Gautam SS, Satsangi GP, Yadav S, Satsangi VR (2018) Application of multiwalled carbon nanotubes on the germination and seedling growth of wheat (Triticum aestivum L). Int J Appl Res 4(11):106–108


Ghodake G, Seo YD, Park D, Lee DS (2010) Phytotoxicity of carbon nanotubes assessed by Brassica Juncea and Phaseolus Mungo. J Nanoelectronics Optoelectron 5(2):157–160. https://doi.org/10.1166/jno.2010.1084


Haghighi M, Silva JATD (2014) The effect of carbon nanotubes on the seed germination and seedling growth of four vegetable species. J Crop Sci Biotechnol 17:201–208. https://doi.org/10.1007/s12892-014-0057-6


Hong CE, Lee JH, Kalappa P, Advani SG (2007) Effects of oxidative conditions on properties of multi-walled carbon nanotubes in polymer nanocomposites. Compos Sci Technol 67(6):1027–1034. https://doi.org/10.1016/j.compscitech.2006.06.003


Hong J, Peralta-Videa JR, Gardea-Torresdey JL (2013) Nanomaterials in Agricultural Production: Benefits and Possible Threats? In: Shamim N, Sharma VK (ed) Sustainable nanotechnology and the environment: advances and achievements, ACS Symposium Series, Vol 1124, edition 1, Chapter 5. American Chemical Society, Washington, DC, pp 73–90. https://doi.org/10.1021/bk-2013-1124.ch005


https://www.fas.usda.gov/data/production/0410000 (USDA Foreign Agricultural Service, U.S. Department of Agriculture). Accessed 14 Feb 2026


Husen A, Siddiqi KS (2014) Carbon and fullerene nanomaterials in plant system. J Nanobiotechnol 12:16. https://doi.org/10.1186/1477-3155-12-16


Ji J, Wang X, Wang G, Zhang J, Song W, Wang R, Ma B, Li T, Guan C (2024) UV-B-priming combined with the soil application of MWCNT enhances rice growth performance under salt stress. J Plant Growth Regul 43:3846–3861. https://doi.org/10.1007/s00344-024-11367-y


Joshi A, Sharma L, Kaur S, Dharamvir K, Nayyar H, Verma G (2020) Plant nanobionic effect of multi-walled carbon nanotubes on growth, anatomy, yield and grain composition of rice. BioNanoScience 10(2):430–445. https://doi.org/10.1007/s12668-020-00725-1


Kam NWS, Dai H (2005) Carbon nanotubes as intracellular protein transporters: generality and biological functionality. J Am Chem Soc 127(16):6021–6026. https://doi.org/10.1021/ja050062v


Khan P, Memon MY, Imtiaz M, Aslam M (2009) Response of wheat to foliar and soil application of urea at different growth stages. Pak J Bot 41(3):1197–1204. https://doi.org/10.3923/ppj.2003.48.55


Khodakovskaya M, Dervishi E, Mahmood M, Xu Y, Li Z, Watanabe F, Biris AS (2009) Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano 3(10):3221–3227. https://doi.org/10.1021/nn900887m


Khodakovskaya MV, De Silva K, Biris AS, Dervishi E, Villagarcia H (2012) Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano 6(3):2128–2135. https://doi.org/10.1021/nn204643g


Khodakovskaya MV, Kim BS, Kim JN, Alimohammadi M, Dervishi E, Mustafa T, Cernigla CE (2013) Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Small 9(1):115–123. https://doi.org/10.1002/smll.201201225


Khot LR, Sankaran S, Maja JM, Ehsani R, Schuster EW (2012) Applications of nanomaterials in agricultural production and crop protection: a review. Crop Prot 35:64–70. https://doi.org/10.1016/j.cropro.2012.01.007


Lahiani MH, Dervishi E, Chen J, Nima Z, Gaume A, Biris AS, Khodakovskaya MV (2013) Impact of carbon nanotube exposure to seeds of valuable crops. ACS Appl Mater Interfaces 5(16):7965–7973. https://doi.org/10.1021/am402052x


Liu Q, Chen B, Wang Q, Shi X, Xiao Z, Lin J, Fang X (2009) Carbon nanotubes as molecular transporters for walled plant cells. Nano Lett 9(3):1007–1010. https://doi.org/10.1021/nl803083u


Matsuzawa Y, Takada Y, Kodaira T, Kihara H, Kataura H, Yoshida M (2014) Effective nondestructive purification of single-walled carbon nanotubes based on high-speed centrifugation with a photochemically removable dispersant. J Phys Chem C 118(9):5013–5019. https://doi.org/10.1021/jp411964z


Miralles P, Johnson E, Church TL, Harris AT (2012) Multiwalled carbon nanotubes in alfalfa and wheat: toxicology and uptake. J Royal Soc Interface 9:3514–3527. https://doi.org/10.1098/rsif.2012.0535


Mondal A, Basu R, Das S, Nandy P (2011) Beneficial role of carbon nanotubes on mustard plant growth: an agricultural prospect. J Nanopart Res 13:4519–4528. https://doi.org/10.1007/s11051-011-0406-z


Morsy M, Helal M, El-Okr M, Ibrahim M (2014) Preparation, purification and characterization of high purity multi-wall carbon nanotube. Spectrochim Acta Part A Mol Biomol Spectrosc 132:594–598. https://doi.org/10.1016/j.saa.2014.04.122


Mukhtar A, Naseer MA, Ali MF, Jabeen S. Beneficial roles of carbon nanotubes in regulation of seed germination physiology. In: Siddiqui MH, Khan MN, Mukherjee S Nanomaterial-plant interactions, carbon nanotubes in agriculture, Academic Press, Elsevier BV (2025) Amsterdam, Netherlands, pp 27–42. https://doi.org/10.1016/B978-0-443-19047-6.00002-3


Nair R, Mohamed MS, Gao W, Maekawa T, Yoshida Y, Ajayan PM, Kumar DS (2012) Effect of Carbon Nanomaterials on the Germination and Growth of Rice Plants. J Nanosci Nanotechnol 12(3):2212–2220. https://doi.org/10.1166/jnn.2012.5775


Panse VG, Sukhatme PV (1954) New Delhi, India. Indian Council of Agricultural Research. 361. https://www.cabidigitallibrary.org/doi/full/10.5555/19561604178


Panse VG, Sukhatme PV (1985) Statistical Methods for Agricultural Workers. ICAR, New Delhi, pp 14–33. https://www.scirp.org/reference/referencespapers?referenceid=1241190


Parisi C, Vigani M, Rodríguez-Cerezo E (eds) (2014) Proceedings of a Workshop on Nanotechnology for the agricultural sector: from research to the field, JRC Research Reports JRC89736, Joint Research Centre. https://data.europa.eu/doi/https://doi.org/10.2791/80497


Patel DK, Kim H-B, Dutta SD, Ganguly K, Lim K-T (2020) Carbon nanotubes-based nanomaterials and their agricultural and biotechnological applications. Materials 13:1679. https://doi.org/10.3390/ma13071679


Pereira TM, da Cunha Neto AR, de Aguila Moreno L, de Oliveira JE, Gomes MP, Nery FC, Carvalho ER, dos Reis MV (2026) Nanopriming with multi-walled carbon nanotubes enhances abiotic stress tolerance in sunflower seeds. Plants 15(4):584. https://doi.org/10.3390/plants15040584


Shang Y, Hasan MK, Ahammed GJ, Li M, Yin H, Zhou J (2019) Applications of nanotechnology in plant growth and crop protection: a review. Molecules 24:2558. https://doi.org/10.3390/molecules24142558


Sharma A, Kothari SL, Kachhwaha S (2023) Impacts of multi-walled carbon-nanotubes on the growth of pearl millet. J Appl Biology Biotechnol 11(4):170–177. https://doi.org/10.7324/JABB.2023.11513


Sharma MMM, Kapoor D, Loyal A, Kumar R, Sharma P, Husen A (2025) Impact of carbon-based nanomaterials on the plant growth-promoting rhizobacteria and sustainable agricultural crop plant production. In: Husen A (ed) Emerging carbon nanomaterials for sustainable agricultural practices. Smart Nanomaterials Technology. Springer, Singapore, pp 317–331. https://doi.org/10.1007/978-981-97-5104-4_16


Sprague GF (1956) Statistical methods for agricultural workers. Agron J 48:323–323. https://doi.org/10.2134/agronj1956.00021962004800070014x


Tiwari D, Cendejas VLM, Borjas SE, Schubert DN, Villagas J, Montoya LM (2014) Interfacing carbon nanotubes (CNT) with plants: enhancement of growth, water and ionic nutrient uptake in maize (Zea mays) and implications for nanoagriculture. Appl Nanosci 4:577–591. https://doi.org/10.1007/s13204-013-0236-7


Tiwari DK, Dasgupta-schubert N, Villaseñor LM (2023) Water absorption kinetics of zea mays seedling using MWCNT as a growth promotor. Microsc Microanal 29:21–23. https://doi.org/10.1093/micmic/ozad067.010


Tripathi S, Sonkar SK, Sarkar S (2011) Growth stimulation of gram (Cicer arietinum) plant by water soluble carbon nanotubes. Nanoscale 3:1176–1181. https://doi.org/10.1039/C0NR00722F


Verma A, Choudhary R, Singh VJ, Kachhwaha S, Kothari SL, Jain R (2025) Assessment of the effect of multi-walled carbon nanotubes (-OH functionalized) on growth characteristics and biochemical profile of Brassica juncea (L.) Czern. & Coss. Environ Sci Pollut Res 32(3):1345–1360. https://doi.org/10.1007/s11356-024-35681-w


Villagarcia H, Dervishi E, Silva KD, Biris AS, Khodakovskaya MV (2012) Surface chemistry of carbon nanotubes impacts the growth and expression of water channel protein in tomato plants. Small 8(15):2328–2334. https://doi.org/10.1002/smll.201102661


Wang M, Sun G, Li G, Hu G, Fu L, Hu S, Yang J, Wang Z, Gu W (2024) Effects of multi walled carbon nanotubes and nano-SiO2 on key enzymes for seed germination and endogenous hormone level in maize seedling. Agronomy 14:2908. https://doi.org/10.3390/agronomy14122908


Wang X, Han H, Liu X, Gu X, Chen K, Lu D (2012) Multi-walled carbon nanotubes can enhance root elongation of wheat (Triticum aestivum) plants. J Nanopart Res 14(6):841. https://doi.org/10.1007/s11051-012-0841-5


Wen S, Li P, Zhao Y, Ran J, Zhang J, Xiao M (2022) Effects of multi-wall carbon nanotubes on seed germination and seedling growth of Water lotus. In: Bin G (ed) Advances in Petrochemical Engineering and Green Development, 1st edn. CRC, London, pp 29–33. https://doi.org/10.1201/9781003318569


Yuan H, Hu S, Huang P, Song H, Wang K, Ruan J, He R, Cui D (2010) Single walled carbon nanotubes exhibit dual-phase regulation to exposed Arabidopsis mesophyll cells. Nanoscale Res Lett 6(1):44. https://doi.org/10.1007/s11671-010-9799-3


Zhu L, Chang DW, Dai L, Hong Y (2007) DNA damage induced by multiwalled carbon nanotubes in mouse embryonic stem cells. Nano Lett 7(12):3592–3597. https://doi.org/10.1021/nl071303v

 


Author Information


Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Agra, India