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Arti Divya, Mehta D. K., Shraddha Shraddha, Singh Jyoti, Nirankar Nirankar
Keywords: Bell pepper, Divergence, Inter and intra cluster distance, PCA, Heat map
This study was carried out during Kharif season 2021 at the Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, HP to study the genetic diversity among 26 genotypes of Bell pepper. Fourteen traits related to growth, yield, yield components, and quality were evaluated. The study involved analysis of intra- and inter-cluster distances, which revealed considerable genetic diversity among the varieties. The greatest inter-cluster distance was found between VI and VII (1004.90), indicating that these varieties were highly divergent. Principal Component Analysis (PCA) identified five major principal components, with PC1 accounting for 26.3% of the total variation. Key traits contributing to variability included fruit weight, fruit breadth, fruit length, pericarp thickness, and number of lobes per fruit. While hierarchical cluster analysis provided clearer differentiation among genotypes groups than PCA, it was less effective in comparison to k-means. Additionally, fruit yield per plant was validated through heat map analysis. The top performing genotypes viz., UHF CAP-1 and UHF CAP-31 were identified promising for future hybridization in bell pepperimprovement. These genotypes, showing superiority for various traits, may be taken into consideration for an efficient hybridization programme in bell pepper.
Anaya-Esparza LM, Mora ZVDL, Vázquez-Paulino O, Ascencio F, Villarruel-López A (2021) Bell peppers (Capsicum annum L.) losses and wastes: source for food and pharmaceutical applications. Molecules 26(17):5341
Anonymous (2016) Package of practices for vegetable crops. Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni Solan, 40
Anuradha, Sood S, Redhu M, Singh R, Kumar N, Loyal A (2024) Genetic diversity study of bacterial wilt-tolerant bell pepper (Capsicum annuum) genotypes using cluster and principal component analysis. Indian J Agric Sci 94(10):1045–1050
Arslan E, Mutlu EG, Dursun O, Bagci SA (2020) Comparative analysis of agronomic traits and ISSR method among some soybeans (Glycine max (L.) Merr.) genotypes. KSU J Agric Nat 23(3):687–696
Arti D, Mehta DK, Vikram A, Kumar R, Aralikatti O (2023) Combining ability and heterosis in bell pepper grown in the north-western hills of India. Indian J. Hortic 80(2): 129–135. https://doi.org/10.58993/ijh/2023.80.2.1
Barik S, Ponnam N, Acharya GC, Singh TH, Dash M, Sahu GS, Mahapatra SK (2021) Genetic variability, character association and diversity studies in Brinjal (Solanum melongena L). Electron J Plant Breed 12(4):1102–1110
Bhakuni V, Shukla PS, Singh K, Singh VK (2017) Morphological characterization and assessment of genetic variability in soybean varieties. Int J Curr Microbiol Appl Sci 6(3):361–369
Bhattarai K, Louws FJ, Williamson JD, Panthee DR (2016) Diversity analysis of tomato genotypes based morphological traits with commercial breeding significance for fresh market production in Eastern USA. Aust J Crop Sci 10:1098
Bozokalfa MK, Esiyok D, Turhan K (2009) Patterns of phenotypic variation in a germplasm collection of pepper (Capsicum annuum L.) from Turkey. Span J Agric Res 7:83–95
Brilhante BD, Santos TD, Santos PH, Kamphorst SH, Neto JD, Rangel LH, Valadares, FV, De Almeida RN, Rodrigues R, Júnior AC, Moulin MM (2021) Phenotypic and Molecular Characterization of Brazilian Capsicum Germplasm. Agronomy 11(5):854. https://doi.org/10.3390/agronomy11050854
Danojevic D, Medic-Pap (2018) Different multivariate analysis for fruit traits in sweet pepper breeding. Genetika 50:121–129. https://doi.org/10.2298/GENSR1801121D
Devi J, Sood S (2018) Genetic Study of Horticultural Traits in Bell Pepper (Capsicum annuum var. grossum) Through Generation Mean Analysis. Agric Res 7:112–119. https://doi.org/10.1007/s40003-018-0298-6
Farebrother RW (2021) Notes on the prehistory of principal components analysis. J Multivar Anal: 104814
Hwang TY, Gwak BS, Sung J, Kim HS (2020). Genetic diversity patterns and discrimination of 172 korean soybean (Glycine max (L.) merrill) varieties based on SSR analysis. Agriculture 10(3):77. https://doi.org/10.3390/agriculture10030077
Karim KMR, Rafii MY, Misran AB, Ismail MFB, Harun AR, Ridzuan RB, Chowdhury MFN, Hossain MM, Yusuff O, Haque MA (2022) Genetic diversity analysis among Capsicum annuum mutants based on Morpho-Physiological and yield traits. Agronomy 12(10):2436. https://doi.org/10.3390/12102436
Kaur G, Joshi A, Jain D, Choudhary R, Vyas D (2016) Diversity analysis of green gram (Vigna radiata (L.) Wilczek) through morphological and molecular markers. Turk J Agric 40(2):229–240
Kousalya R, Praneetha S, Irene VP, Ravichandran V, Iyankar K, Varanavasiappan S (2024) Unveiling the genetic variability, character association and principal component analysis for yield and yield contributing traits in Brinjal (Solanum melongena) genotypes. Indian J Agric Sci 94(10):1039–1044
Mahalanobis (1936) On the generalized distance in statistics. Proc Natl Inst Sci (India) 2(1):49–55
Malik R, Tiwari R, Arora A, Kumar P, Sheoran S (2013) Genotypic characterization of elite Indian wheat genotypes using molecular markers and their pedigree analysis. Aust J Crop Sci 7(5):561–567
Misra S, Lal R, Darokar M, Khanuja S (2011) Genetic Variability in Germplasm Accessions of Capsicum annuum L. Am. J. Plant Sci 2:629–635. http://dx.doi.org/10.4236/ajps.2011.25074
Mukuze C, Tukamuhabwa P, Maphosa M, Dari S, Dramadri I.O, Obua T, Kongai H, Rubaihayo P (2020) Genetic diversity analysis among soybean genotypes using SSR markers in Uganda. Afr. J. Biotechnol 19:439–448. https://doi.org/10.5897/AJB2020.17152
Naik SM, Madhusudan K, Motagi BN, Nadaf HL (2016) Diversity in soybean (Glycine max L.) accessions based on morphological characterization and seed longevity characteristics. Int J Progressive Res 11(3):377–381
Rana S, Kumar P, Sharma P, Singh A, Upadhyay SK (2015) Evaluationof different rootstocks for bacterial wilt tolerance in Bell pepper [(Capsicum annuum (L.) Var. grossum (Sendt.)] under protected conditions. Himachal J Agri Res 41(1):100–103. http://www.hjar.org/index.php/hjar/article/view/110695
Ranganna S (1986) Handbook of analysis and quality control for fruit and vegetable products.2nd ed. Tata McGraw Hill, New Delhi. pp.105–506
Razzaq A, Khan TM, Saeed A, Kamaran S, Zeb A (2016) Genetic diversity and association analysis for different morphological traits in Capsicum annuum. Int J Biomol Biomed 5(1):20–28
Rencher AC (2002) Methods of multivariate analysis. Wiley Series in Probability and Mathematical Statistics, 2nd edn. Willey Inter science, New York
Sen N, Biswas K, Sinha SN (2021) Assessment of genetic divergence through cluster analysis of Chilli varieties. J Biosci 46:52. https://doi.org/10.1007/s12038-021-00167-1
Singh B, Chaubey T, Singh RK, Upadhyay DK, Jha A, Pandey S (2024) Genetic variation in phenatic traits of extant varieties of Brinjal (Solanum melongena L). J Appl Hortic 26(1):107–111
Singh RK, Chaudhary BD (1997) Biometrical methods in quantitative genetics analysis. Kalyani, New Delhi. 215 – 18
Sood T, Sood S, Sood VK, Badiyal A, Anuradha, Kapoor S, Sood V, Kumar N (2023) Characterisation of bell pepper (Capsicum annuum L. var. Grossum Sendt.) accessions for genetic diversity and population structure based on agro-morphological and microsatellite markers. Sci Hortic 321. https://doi.org/10.1016/j.scienta.2023.112308
Sood S, Jambhulkar S, Singh Y, Gupta N, Sharma, S (2017) Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum). J. Appl. Nat. Sci. 9(2)
Tsonev S, Todorova V, Groseva S, Popova T, Todorovska EG (2017) Evaluation of diversity in Bulgarian pepper cultivars by agronomical traits and ISSR markers. Genetika 49(2):647–662
Division of Vegetable Science, ICAR- Indian Agricultural Research Institute, Pusa, India