Growth promotion ability of endophytic Aspergillus niger on different species of Vigna

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00570-z
First Page: 192
Last Page: 201
Views: 1330

Keywords: Benzoic acid, Chlorophyll, GC/MS, Pulses, Secondary metabolites, Volatile organic compounds


Fungal endophytes ubiquitously colonize plants’ internal tissues, and the chemical interaction between the host plant and the residing endophyte has a remarkable influence on plant growth. Analysis of impact of endophytic Aspergillus niger on Vigna radiata, V. mungo and V. aconitifolia is discussed in this study. GC-MS analysis of the endophyte extract showed the presence of secondary metabolites such as volatile organic compounds (VOCs), benzoic acid, and other phenolics. The extract of the endophyte showed significant improvement in root length, shoot length, and root number of all Vigna seedlings. The highest improvement in shoot length (6.04 ± 1.07 cm) and root length (9.19 ± 0.96 cm) was observed in V. aconitifolia with maximum root number in V. radiata (18.4 ± 1.68). Pot assays were performed further to assess the endophyte’s potential in the field. The co-culturing of endophytes with the plant in pot assays also showed significant enhancement in root length (7.77 ± 1.31 cm) and root number (15.46 ± 4.27, p < 0.001) in V. mungo. The shoot length of endophyte colonized plants showed 66%, 102%, and 33% increase in V. radiata, V. Mungo, and V. aconitifolia, respectively. These results indicate that the endophytic A. niger facilitates plant growth and improve the plant’s physiological features by producing certain bioactive compounds. The results also suggest that colonization of A. niger endophyte can aid/contribute in sustainable agricultural practices.

Benzoic acid, Chlorophyll, GC/MS, Pulses, Secondary metabolites, Volatile organic compounds

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Abdel-Hamid M, Fouda A, El-Ela H, El-Ghamry A, Hassan S (2021) Plant growth-promoting properties of bacterial endophytes isolated from roots of Thymus vulgaris L. and investigate their role as biofertilizers to enhance the essential oil contents. Biomol Concepts 12(1):175–196

Abouziena H, Amin AA, Abd El-Kader AA, El-Awadi M, Gharib FAE (2016) Effects of benzoic acid and thiourea on growth and productivity of wheat (Triticum aestivum L.) plants. Ponte Acad J 72(4).

Ajilogba CF, Babalola OO (2019) GC–MS analysis of volatile organic compounds from Bambara groundnut rhizobacteria and their antibacterial properties. World J Microbiol Biotechnol 35(6):83

Anjum SA, Ehsanullah Xue L, Wang L, Saleem MF, Huang CJ (2013) Exogenous benzoic acid (BZA) treatment can induce drought tolerance in soybean plants by improving gas-exchange and chlorophyll contents. Aust J Crop Sci 7(5):555–560

Arnon D (1949) Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15

Bailly A, Weisskopf L (2014) The modulating effect of bacterial volatiles on plant growth. Plant Signal Behav 7:79–85

Bayat F, Mirlohi A, Khodambashi M (2009) Effects of endophytic fungi on some drought tolerance mechanisms of tall fescue in a hydroponics culture. Russian J Plant Physiol 56(4):563–570

Benavides-Mendoza A, Burgos-Limón D, Ramírez H, Robledo-Torres V, Sandoval-Rangel A (2012) Benzoic acid effect in the growth and yield of tomato in calcareous soil. Acta Hortic 938:251–256

Bezerra JDP, Santos MGS, Svedese VM, Lima DMM, Fernandes MJS, Paiva LM, Souza-Motta CM (2012) Richness of endophytic fungi isolated from Opuntia ficus-indica Mill. (Cactaceae) and preliminary screening for enzyme production, World J Microbiol Biotechnol 28:1989–1995

Bhardwaj A, Sharma D, Jadon N, Agrawal PK (2015) Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus roxburghii. Arch Clin Microbiol 6(3):1

Chen ZX, Zhou BL, Du L, Ye XL (2012) Effects of phenol 2,4-bis(1,1-dimethylethyl) on seedling growth of eggplant and verticillium dahlia. Allelopathy J 30(1):81–92

Cheng F, Cheng Z (2015) Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Front Plant Sci 6:1020. DOI:

Chitnis VR, Suryanarayanan TS, Nataraja KN, Prasad SR, Oelmuller R, Shaanker RU (2020) Fungal endophyte-mediated crop improvement: the way ahead. Front Plant Sci 11:1588

Dastogeer KMG (2018) Influence of fungal endophytes on plant physiology is more pronounced under stress than well-watered conditions: a meta-analysis. Planta 248:1403–1416

El-Hawary SS, Moawad AS, Bahr HS, Abdelmohsen UR, Mohammed R (2020) Natural product diversity from the endophytic fungi of the genus aspergillus. RSC Adv 10:22058–22079

Fariduddin Q, Hayat S, Ahmad A (2003) Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity and seed yield in Brassica juncea. Photosynthetica 41:281–284

Fracheboud Y, Jompuk C, Ribaut JM, Stamp P, Leipner J (2004) Genetic analysis of cold-tolerance of photosynthesis in maize. Plant Mol Biol 56:241–253

Glick BR (2012) Plant growth-promoting bacteria: mechanisms and applications.Scientifica Article ID963401

Glick BR (2015) Beneficial plant-bacterial interactions. Springer, Heidelberg

Guo PG, Li RH (2000) Effects of high nocturnal temperature on photosynthetic organization in rice leaves. J Integr Plant Biol 42(7):673–678

Hamayun M, Hussain A, Khan SA, Kim HY, Khan AL, Waqas M, Irshad M, Iqbal A, Rehman G, Jan S, Lee IJ (2017) Gibberellins producing endophytic fungus Porostereum spadiceum AGH786 rescues growth of salt affected soybean. Front Microbiol 8:686. DOI:

Jishma P, Hussain N, Chellappan R, Rajendran R, Mathew J, Radhakrishnan EK (2017) Strain-specific variation in plant growth promoting volatile organic compounds production by five different Pseudomonas spp. as confirmed by response of Vigna radiata seedlings. J Appl Microbiol. doi:

Kedar A, Rathod D, Yadav A, Agarkar G, Rai M (2014) Endophytic Phoma sp. isolated from medicinal plants promotes the growth of Zea mays. Biosci 6:132–139

Khan AL, Hamayun M, Kim YH, Kang SM, Lee JH, Lee IJ (2011) Gibberellins producing endophytic aspergillus fumigatus sp. LH02 influenced endogenous phytohormonal levels, isoflavonoids production and plant growth in salinity stress. Process Biochem 46(2):440–447

Khan AL, Hamayun M, Kang SM, Kim YH, Jung HY, Lee JH, Lee IJ (2012) Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiol 12:3.

Khan AL, Waqas M, Hamayun M, Al-Harrasi A, Al-Rawahi A, Lee IJ (2013) Co-synergism of endophyte penicillium resedanum LK6 with salicylic acid helped Capsicum annuum in biomass recovery and osmotic stress mitigation. BMC Microbiol 13:51.

Khan AR, Ullah I, Waqas M, Shahzad R, Hong S, Park G, Jung BK, Lee I, Shin J (2015) Plant growth promoting potential of endophytic fungi isolated from Solanum nigrum leaves. World J Microbiol Biotechnol 31(9):1461–1466

Mathur P, Ghosh U, Chetani R, Mehtani P, Sharma C, Bhatnagar P (2022a) Enzymatic bioprospection of endophytic aspergillus niger isolated from Albizia lebbeck (L.) Benth. S Afr J Bot 148:580–587

Mathur P, Mehtani P, Sharma C, Bhatnagar P (2022b) Impact of pertinent endophytic fungal communities and their secondary metabolites on crops. In: Singh J and Sharma D (Eds.), Microbial resource technologies for sustainable development, Elsevier, pp 147–156

Mathur P, Chaturvedi P, Sharma C, Bhatnagar P (2022c) Improved seed germination and plant growth mediated by compounds synthesized by endophytic aspergillus niger (isolate 29) isolated from Albizia lebbeck (L.) Benth. 3 Biotech 12(10):271

Pablo CHD, Pagaduan JRR, Langres HKC, Hipol RM (2020) Plant growth-promoting characteristics of root fungal endophytes isolated from a traditional Cordillera rice landrace. Stud Fungi 5(1):536–549

Rosenberg E, Zilber-Rosenberg I (2018) The hologenome concept of evolution after 10 years. Microbiome 6:78. 10.1186/ s40168-018-0457-9

Schlatter D, Kinkel L, Thomashow L, Weller D, Paulitz T (2017) Disease suppressive soils: new insights from the soil microbiome. Phytopathology 107(11):1284–1297

Tawfike AF, Tate R, Abbott G, Young L, Viegelmann C, Schumacher M, Diederich Marc, Edrada-Ebel RA (2017) Metabolomic tools to assess the chemistry and bioactivity of endophytic aspergillus strain. Chem Biodiver 14(10):e1700040. DOI:

Wijesooriya WADK, Deshappriya N (2016a) An inoculum of endophytic fungi for improved growth of a traditional rice variety in Sri Lanka. Trop Plant Res 3(3):470–480

Wang Y, Dai CC, Cao JL, Xu DS (2012) Comparison of the effects of fungal endophyte Gilmaniella sp. and its elicitor on Atractylodes lancea plantlets. World J Microbiol Biotechnol 28:575–584

Waqas M, Khan AL, Hamayun M, Kamran M, Kang S, Kim Y, Lee J (2012a) Assessment of endophytic fungi cultural filtrate on soybean seed germination. Afr J Biotechnol 11(85):15135–15143

Waqas M, Khan AL, Kamran M, Hamayun M, Kang S, Kim Y, Lee J (2012b) Endophytic fungi produce gibberellins and indole acetic acid and promotes host-plant growth during stress. Molecules 17:10754–10773

Wijesooriya W, Deshappriya N (2016b) An inoculum of endophytic fungi for improved growth of a traditional rice variety in Sri Lanka. Department of Botany, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka. Trop Plant Res. DOI: 10.22271/tpr.2016b.v3.i3.063

Zhang H, Xie X, Kim MS, Kornyeyev DA, Holaday S, Pare PW (2008) Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta. Plant J 56:264–273



Author Information

Mathur Parikshana
Department of Biotechnology, IIS (Deemed to be University), Jaipur, India