Growth promotion ability of endophytic Aspergillus niger on different species of Vigna


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00570-z
First Page: 192
Last Page: 201
Views: 1330


Keywords: Benzoic acid, Chlorophyll, GC/MS, Pulses, Secondary metabolites, Volatile organic compounds


Abstract


Fungal endophytes ubiquitously colonize plants’ internal tissues, and the chemical interaction between the host plant and the residing endophyte has a remarkable influence on plant growth. Analysis of impact of endophytic Aspergillus niger on Vigna radiata, V. mungo and V. aconitifolia is discussed in this study. GC-MS analysis of the endophyte extract showed the presence of secondary metabolites such as volatile organic compounds (VOCs), benzoic acid, and other phenolics. The extract of the endophyte showed significant improvement in root length, shoot length, and root number of all Vigna seedlings. The highest improvement in shoot length (6.04 ± 1.07 cm) and root length (9.19 ± 0.96 cm) was observed in V. aconitifolia with maximum root number in V. radiata (18.4 ± 1.68). Pot assays were performed further to assess the endophyte’s potential in the field. The co-culturing of endophytes with the plant in pot assays also showed significant enhancement in root length (7.77 ± 1.31 cm) and root number (15.46 ± 4.27, p < 0.001) in V. mungo. The shoot length of endophyte colonized plants showed 66%, 102%, and 33% increase in V. radiata, V. Mungo, and V. aconitifolia, respectively. These results indicate that the endophytic A. niger facilitates plant growth and improve the plant’s physiological features by producing certain bioactive compounds. The results also suggest that colonization of A. niger endophyte can aid/contribute in sustainable agricultural practices.


Benzoic acid, Chlorophyll, GC/MS, Pulses, Secondary metabolites, Volatile organic compounds


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Acknowledgements



Author Information


Mathur Parikshana
Department of Biotechnology, IIS (Deemed to be University), Jaipur, India