Harnessing the saline soil-inhabiting bacteria for antagonistic, antibiotic resistance, and plant growth-promoting attributes

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00466-4
First Page: 907
Last Page: 919
Views: 1508

Keywords: Arnowu0027s assay, Csaky assay, Salt, S. oryzae , Vancomycin


Biological control of soil-borne plant infections is sorely needed since they are challenging to treat with conventional fungicides. This fundamental research was aimed to find bacteria that live in saline soils (halophilic or halotrophic) and have antagonistic activity against Sclerotium oryzae and Ralstonia solani. A total of fifty bacterial strains were recovered from the saline soils of Gangavathi, Karnataka, India. Eight of the fifty bacterial strains in the dual culture approach demonstrated antibiosis against S. oryzae and R. solani. The isolates that produced HCN, siderophore, and hydrolytic enzymes strongly correlated with their biocontrol activity. Arnow’s and Csaky’s methods were used for testing the types of siderophores formed, and only a few isolates produced both catecholate and hydroxamate types. In Arnow’s and Csaky’s assays, the isolate Bacillus albus (HB-17) showed catechol and hydroxamate type siderophores. These prospective isolates’ identity was previously confirmed using 16 S rRNA analysis of Bacillus cereus, Bacillus albus, Bacillus safensis, Staphylococcus xylosus, Lysinibacillus sphaericus, and Pseudomonas stutzeri. Antibiotic resistance testing revealed that the isolates were resistant to Penicillin, Ofloxacin, and Vancomycin. Furthermore, the strains were tested for their plant growth-promoting properties, such as phosphorous (P) and zinc (Zn) solubilization, and potassium (K) release, at three NaCl concentrations (3, 6, and 10% w/v). At a concentration of 6% NaCl, the solubilization zones of zinc carbonate (ZnCO3), zinc oxide (ZnO), and phosphorous (Ca3PO4) were 5.20–9.0 mm, 6.06–13.20 mm, and 6.30–9.70 mm, respectively. Finally, we investigated the isolation of new bacterial strains with antifungal activity from saline soils in Gangavathi, Karnataka, India’s untapped agro-ecological habitats. This research also provides the path for further characterization of these isolates to maximize their antifungal potential.

Arnowu0027s assay, Csaky assay, Salt, 
              S. oryzae
            , Vancomycin

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Author Information

Nagaraju Yalavarthi
University of Agricultural Sciences, Raichur, India
University of Agricultural Sciences, Raichur, India

Gowder S. B.
College of Agriculture, Gangavathi, India