Herbal antibacterial remedy against upper respiratory infection causing bacteria and in vivo safety analysis


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00281-3
First Page: 264
Last Page: 268
Views: 1086

Keywords: Streptococcus pyogenes, Haemophilus influenza, Staphylococcus aureus , Upper respiratory tract infections, Antibacterial, Acute oral toxicity


In the present study, the antibacterial activity and in vivo acute toxicity of five different extracts was analyzed. The Minimum Inhibitory Concentration and antibacterial activity of five different extracts, including Terminalia chebula, Camellia sinensis, Glycyrrhiza glabra, Vitis vinifera, and Terminalia arjuna, against three different bacterial species, namely, Streptococcus pyogenes, Haemophilus influenza, and Staphylococcus aureus was analyzed by broth dilution method and agar well diffusion method, respectively. The in vivo acute oral toxicity was analyzed by using Wistar rats. The antibacterial activity of different extracts was calculated by analyzing the zone of inhibition, and the maximum zone of 22 mm and minimum zone of 10 mm were observed against the different upper respiratory infections causing bacteria. The lowest MIC (0.8 mg/ml) found was of Camellia sinensis against the Streptococcus pyogenes and Staphylococcus aureus. No sign of toxicity and mortality was observed during the 14 days of study. The in vitro results of the tested extracts indicate that they possessed antibacterial activity and were found non-toxic. So, it can be used as an alternative for the treatment of upper respiratory infection-causing bacteria.

                Streptococcus pyogenes, Haemophilus influenza, Staphylococcus aureus
              , Upper respiratory tract infections, Antibacterial, Acute oral toxicity

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We are thankful to TEQIP for their support and the United Institute of Pharmacy (UIP) for allowing us to use their animal house facility. The authors acknowledge the Indian Council of Medical Research (ICMR) (AMR/ADHOC/184/2019-ECD-II) and DST-FIST grant to execute the work.

Author Information

Singh Devendra
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India

Agarwal Vishnu
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India