Keywords: Browning, Callus, Glycyrrhiza glabra L., Licorice, Phytohormone, Regeneration
Glycyrrhiza glabra, member of Leguminosae family, is economically valuable due to its medicinal properties. Today it is considered as an endangered plant, therefore establishing a rapid and efficient system for micropropagation of this plant is desirable for their protection. The present study standardized the micropropagation protocol for G. glabra involving callus induction and shoots regeneration with explants viz. leaf and stem. Our finding suggests that the use of only one sterilant is not efficient for the sterilization of licorice explant. HgCl2 (0.1%) for 5 min and ethanol (70%) for 5 min was found best when used along with prior washing and surface sterilization with tween-20 and bavistin. Callus induction was achieved on MS medium containing various concentration of BAP, 2,4-D and NAA. Maximum callus induction was observed on MS medium containing 2 mg/l BAP, 0.5 mg/l 2,4-D and 50 mg/l ascorbic acid with both leaf and stem explants. Production of callus using leaf as an explant was better than that of stem. The development of shoots from callus was initiated on MS medium containing BAP and IAA. The most efficient shoot formation was observed with 4 mg/l BAP, 0.2 mg/l IAA and 60 mg/l ascorbic acid. Best rooting was recorded with 3 mg/l IBA. Histological aspect of indirect organogenesis of G. glabra were evaluated. Successful multiplication of shoots from explants leads to the production of improved quality and great quantity of planting material.
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This study was supported and financed by Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, India.