Identification of molecular marker associated with mungbean yellow mosaic virus resistance in mungbean [Vigna radiata (L.) Wilczek]


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00063-y
First Page: 532
Last Page: 539
Views: 515


Keywords: Vigna radiata (L.) Wilczek, Bulked segregant analysis, Molecular marker, RAPD, Mungbean yellow mosaic virus (MYMV)


Abstract


Mungbean [Vigna radiata (L.) Wilczek] is an important legume which can be grown in varying environmental conditions, during all three crop seasons viz., kharif, rabi and spring/summer in India, as sole or inter crop for grain and green manure. It is an excellent source of easily digestible proteins with low flatulence, which complements the staple rice diet in Asia. Mungbean Yellow Mosaic Disease (MYMD) caused by whitefly (Bemisia tabaci) transmitted mungbean Yellow Mosaic Virus (MYMV) is an important constraint of mungbean. To fulfill future demands, there is a need to use molecular marker technology and other biotechnological interventions. Hence, Bulked Segregant Analysis (BSA) with a Random Amplified Polymorphic DNA (RAPD) marker technique was used to analyze the F2:3 individuals of Pusa Ratna (susceptible) × Meha (resistant) to identify the molecular marker linked to MYMV resistance in mungbean. Field screening of thirty-five mungbean genotypes against MYMV was carried out, prior to crossing program to identify the resistant and susceptible genotypes. The RAPD primer, OPP 07 showed the specific band of 895 bp in resistant parent and their bulks, but not in the susceptible parent and their bulks. Co-segregation analysis was performed in resistant and susceptible F2:3 individuals; it confirmed that OPP 07895 marker was associated with MYMV resistance in mungbean. The linked RAPD molecular marker OPP 07895, can be used for the identification of (Quantitative Trait Locus) QTL for MYMV resistance and marker assisted selection program in mungbean.


Vigna radiata (L.) Wilczek, Bulked segregant analysis, Molecular marker, RAPD, Mungbean yellow mosaic virus (MYMV)


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Acknowledgements


Authors are grateful to the authorities of Pulses Research Station and C. P. College of Agriculture, SDAU, Sardarkrushinagar, India, for providing facilities to conduct the research work.


Author Information


Dharajiya Darshan T.
Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture (CPCA), Sardarkrushinagar Dantiwada Agricultural University (SDAU), Palanpur, India
darshanbiotech1@gmail.com