Impact of the saffron extract on growth and antioxidant enzymes activity of Solanum lycopersicum L. seedlings in Morrocan open field conditions

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-021-00238-6
First Page: 555
Last Page: 567
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Keywords: Biostimulant, n Crocus sativus L., Enzyme activities, Oxidative stress, Tomato


An open field experiment was carried out to assess the effect of aqueous saffron extract (ASE) by fertigation and foliar spray as a biostimulant in tomato seedlings growth and physiological indices. The ASE application by both methods influenced the plants height compared to control. However, the application of the extract at high concentrations negatively affected some of the parameters studied, such as an increase of hydrogen peroxide (H2O2) and malondialdehyde (MDA) content, indicating oxidative stress in tomato plants. Nevertheless, the catalase (CAT) and ascorbate peroxidase (APX) enzymes showed an increased activity response to varying concentrations of ASE. However, the application method had a significant effect only on the H2O2 and APX content. Our studies on ASE in treated plants suggest that ASE could improve certain physiological aspects of the recipient plants and a biological stimulator.

Biostimulant, n                     Crocus sativus L., Enzyme activities, Oxidative stress, Tomato

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Author Information

Amine Khoulati
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco
Ennouamane Saalaoui
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco

Oussama Bekkouch
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco

Samira Mamri
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco

Mohammed Choukri
Laboratory of Biochemistry, University Hospital Center Mohammed VI, Oujda, Morocco

Souliman Amrani
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco

Abdeslam Asehraou
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco

Ennouamane Saalaoui
Laboratory of Biochemistry and Biotechnology, Faculty of Sciences, Mohammed First University, Oujda, Morocco