Vegetos

Abstract

Chlorophyta is used as a model organism to understand physiological and biological activities of plant cells. Ascorbate peroxidase (APX) and Glutathione peroxidase (GPX) are scavenging enzymes to reduce lipid hydroperoxides and free H₂O₂ and thus prevent potential H₂O₂ induced cellular damage. In the present study; we performed in-silico comparative evaluation of GPX and APX of 7 Chlorophytes to analyse their natural and complex regulation.

We analysed the exon/intron organization by Gene Structure Display Server 2.0, physiochemical properties by ProtParam tool, conserved motif signatures by Motif Elicitation tool and phylogenetic relationships by Molecular Evolutionary Genetics Analysis 6.0 tool. We generated 3D models of GPX and APX homolog sequences by Phyre2 server and protein-protein interaction networks among the GPX and APX homolog sequences by Cytoscape. The GPX and APX gene expression profiles were studied by utilizing Genevestigator database.

Out of the 7 species; in 5 species we found 9 GPX homolog sequence. 19 homolog sequences of APX were found in all 7 species. GPX and APX homologs belonged to GSHPx (PF00255.18) and peroxidase (PF00141.22) protein domain families respectively. We found exon in GPX homolog ranging from 1–10 nos. and in APX from 1–11 nos. The sequence length, molecular weight and theoretical isoelectric point (pI) values were determined. The potential expression profile of GPX and APX genes were analysed at 105 anatomical parts and 10 developmental stage levels by comparing with A rabidopsis thaliana. Analysed GPX and APX sequences were expressed in 105 anatomical tissues and 10 developmental stages in A. thaliana.