Vemula Suresh, Koppula Thirupathi, Jogam Phanikanth, Mohammed Mustafa
Keywords: Corallocarpus epigaeus , Nodal explants, Micropropagation, ISSR, Genetic fidelity
Corallocarpus epigaeus (Rottler) Hook.f. is an endangered tuberous medicinal climber of family Cucurbitaceae. Despite high medicinal value, over-exploitation made it threatened. In vitro propagation has been adopted for conserving this endangered medicinal plant. Direct shoots induction was achieved from nodal explants on MS medium fortified with various concentrations of BAP and TDZ individually and BAP + IAA, TDZ + IAA, BAP + l-glutamic acid and TDZ + l-glutamic acid combinations. The highest frequency of multiple shoots (43.33 ± 0.53) was achieved on MS medium fortified with 1.5 mg/l TDZ + 1.5 mg/l IAA from nodal explants but shoot length (12.9 ± 0.15 cm) was high on MS medium supplemented with 1.0 mg/l TDZ and 2.0 mg/l l-Glutamic acid. The highest percentage (78%) of rooting was achieved on half strength MS medium augmented with 1.0 mg/l IBA with a mean number of roots 10.76 ± 0.30 cm, an average root length is 1.69 ± 0.07 cm. Rooted plantlets were acclimatized in the greenhouse and successfully transplanted to natural conditions with a 68% survival rate. ISSR markers were used to check the genetic fidelity between in vivo and in vitro developed plantlets. The results indicated that the micropropagated plants are monomorphic and true type when compare with mother plant.
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The authors are grateful to Prof. Sadanandam Abbagani Formar Dean Faculty of Sciences and Dr. A. V. Rao Department of Biotechnology, Kakatiya University for their valuable suggestions and encouragement. The authors are thankful to Dr. T. Christopher Reuben Head, Department of Botany, Dr. T. Shasthree Head, Department of Biotechnology, Kakatiya University, warangal for facilities and Mr. Ramesh Kandagatla for his help with setting the plant images.