In vitro micropropagation and genetic fidelity assesment in Flemingia macrophylla (Willd.) Merr: an ethnomedicinal plant

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-020-00106-9
First Page: 286
Last Page: 295
Views: 1703

Keywords: Flemingia macrophylla , Micro-propagation, ISSR, RAPD


Flemingia macrophylla is an important ethnomedicinal plant of Fabaceae, with immense pharmaceutical properties. Now, it is becoming rare because of its poor seed dispersal mechanism and over-exploitation of the material. In this study we have successfully established an in vitro micro-propagation protocol employing explants like ex vivo leaf and cotyledonary node. Both the explants were cultured on MS medium supplemented with various combinations and concentrations of growth hormones. In vitro morphogenetic response of leaf explants are very poor when compared with cotyledonary nodes. Cotyledonary nodes cultured on MS medium supplemented with BAP (2.0 mg/l) in combination with IAA (0.2 mg/l) produced a high percentage of multiple shoots (13.76 ± 1.1). Elongated shoots (6.30 ± 0.01 cm) rooted on MS medium with IBA (0.8 mg/l) produced a high frequency of rooting (10.80 ± 1.23) with excellent root length (10.56 ± 1.70 cm). Plantlets with a fully developed root system were acclimatized using soil: manure (1:1) and shifted to the greenhouse. Investigations for the corroboration of genetic stability among the clones and mother plants were carried out by using ISSR and RAPD markers and the results were in clear evidence that all the plants are true-to-type. This scientific approach for in vitro propagation and conservation is prerequisite for better sustainability of the plant.

                Flemingia macrophylla
              , Micro-propagation, ISSR, RAPD

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Authors are greatly acknowledged to Prof. A. Sadanandam CSIR-Emeritus Scientist, Department of Biotechnology, Kakatiya University for his kind advice and encouragement. We are greatly acknowledged to Prof. V. S. Raju Department of Botany, Kakatiya University for authentication of plant and encouragement. Authors are also greatful to UGC, New Delhi for sanctioning SAP DRS programme to the department.

Author Information

Sirikonda Abhiteja
Department of Biotechnology, Kakatiya University, Warangal, India

Jogam Phanikanth
Department of Biotechnology, Kakatiya University, Warangal, India

Ellendula Raghu
Department of Biotechnology, Kakatiya University, Warangal, India

Kudikala Hemalatha
Department of Biotechnology, Kakatiya University, Warangal, India

Mood Kasim
Department of Biotechnology, Kakatiya University, Warangal, India

Allini Venkateswar Rao
Department of Biotechnology, Kakatiya University, Warangal, India