Keywords: Explants, Organogenesis, Somatic embryogenesis, Thidiazuron
Crataeva nurvala (Buch Ham) is a medicinally important tree. The root and stem bark of the tree are the main ingredients of various pharmaceutical products. The use of natural propagation strategies for C. nurvala has proven difficult. In this study, callus cultures were established from leaflets, petal, petiole, internodes and nodes on Murashige and Skoog’s (MS) medium supplemented with 2 mg L−1 of 2,4-dichlorophenoxyacetic acid and 0.4 mg L−1 kinetin. Transfer of calli on MS supplemented with 0.1-0.5 mg L−1 of kinetin, N6-benzyl adenine, or thidiazuron or N6-(2-isopentenyl) adenine resulted in root and shoot bud differentiation and somatic embryogenesis. In vitro regenerated shoots were successfully rooted on half-strength MS medium containing 0.5 mg L−1 α- naphthalene acetic acid. Somatic embryos continued their differentiation and maturation and converted into plantlets on medium without plant growth regulators. The regeneration response, the type of morphogenesis, varied with callus source and plant growth regulators. Micropropagated plantlets of C. nurvala successfully acclimatized in natural condition.
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