Vegetos
(An International Journal of Plant Research & Biotechnology)
In vitro regeneration of Ipomoea purpurea (L.) Roth via indirect organogenesis from seedling-derived internode explants
*Article not assigned to an issue yet
Anbazhakan Rengasamy, Li Neng-Qi-, Poudel Brihaspati, Gao Jiang-Yun-
Research Articles | Published: 09 April, 2025
First Page: 0
Last Page: 0
Views: 279
Keywords: n Ipomoea purpurean , Organogenic callus, Plant regeneration, Seedling explants
Abstract
Ipomea purpurea (L.) Roth. commonly known as Morning Glory is widely recognised as commercial, ornamental, and medicinal plant for its ethnomedicinal properties. This plant has been used in traditional medicines to cure a variety of ailments throughout the world. An effective and reproducible plant regeneration method has been developed for I. purpurea from in vitro explants. Callus has been induced from 78.33% internode explants excised from one-week old seedlings on Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.5 mg/l. Callus proliferation was achieved using a combination of 1.5 mg/l 2,4-D and 0.5 mg/l NAA from internode explants. Adventitious shoots were successfully regenerated from callus on MS medium with 1.0 mg/l thidiazuron (TDZ). Shoot proliferation was achieved with 1.0 mg/l TDZ and 0.5 mg/l NAA, showing a 98% response. The regenerated shoots developed roots on half-strength MS medium with 0.5 mg/l indole-3-butyric acid (IBA). The rooted plantlets were then transferred to a peat, vermiculite, and perlite mix (1:1:1) and had an over 85% survival rate within a month. This method can produce hundreds of plants and support genetic studies in Ipomoea species.
References
Acemi A, Bayrak B, Çakır M, Demiryürek E, Gün E, El Gueddari NE, Özen F (2018) Comparative analysis of the effects of Chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. Vitro Cell Dev Biol Plant 54:537–544
Acemi RK, Acemi A (2019) Polymerization degree-dependent changes in the effects of Chitosan treatments on photosynthetic pigment, protein, and dry matter contents of. Euro Biotech J 34:197–202
Ahmad W, Zahir A, Nadeem M, Zia M, Hano C, Abbasi BH (2019) Thidiazuron-induced efficient biosynthesis of phenolic compounds in callus culture of Ipomoea turbinata Lagasca and Segura. Vitro Cell Dev Biol Plant 55:710–719
Alula K, Zeleke H, Manikandan M (2018) In vitro propagation of sweet potato (Ipomoea Batatas (L.) Lam) through apical meristem culture. J Pharmacogn Phytochem 7 1:2386–2392
Chandler JM, Munson RL, Vaughan CE (1977) Purple Moonflower: emergence, growth, reproduction. Weed Sci 25 2:163–167
Chaves VTA, Chaves ACTA, Filho RS L. C., Oliveira LMD (2024) In vitro establishment and morphogenesis of Operculina macrocarpa L. Urb (Convolvulaceae). Ciência Rural 54–12:e20220701
Cheruvathur MK, Abraham J, Thomas TD (2015) In vitro micropropagation and flowering in Ipomoea sepiaria Roxb. An important ethnomedicinal plant. Asian Pac J Reprod 4(1):49–53
Gunn CR (1969) January) History and taxonomy of the purple moonflower, Ipomoea turbinata Lagasca y Segura. In Proceedings of the Association of Official Seed Analysts 116–123
Ishikuro H, Nitasaka E, Otani M (2014) An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I. tricolor. Plant Biotechnol 31 2:179–183
Islam MS, Bari MA (2013) Rapid in vitro multiplication, callogenesis and indirect shoot regeneration in Ipomoea mauritiana-a rare medicinal plant in Bangladesh. Med Arom Plant 2:138–140
Islam S, Chawdhury MHR, Hossain I, Sayeed SR, Rahman S, Azam FS, Rahmatullah M (2014) A study on callus induction of Ipomoea Mauritiana: an ayurvedic medicinal plant. American-Eurasian J Sustainable Agric, 86–94
Jayasuriya KG, Baskin JM, Baskin CC (2008) Dormancy, germination requirements and storage behaviour of seeds of convolvulaceae (Solanales) and evolutionary considerations. Seed Sci Res 18 4:223–237
Jha P, Norsworthy JK, Kumar V, Reichard N (2015) Annual changes in temperature and light requirements for Ipomoea purpurea seed germination with after-ripening in the field following dispersal. Crop Prot 67:84–90
Kirtikar KR, Basu BD (1975) Indian medicinal plants I-IV. Bishen Singh Mahendra Pal Singh, new Connaught place. Dehrahun 2:754
Meira M, Silva EPD, David JM, David JP (2012) Review of the genus Ipomoea: traditional uses, chemistry, and biological activities. Rev Bras Farmacogn 22:682–713
Mungole A, Awati R, Dey S, Chaturvedi A, Zanwar P (2009) In-vitro callus induction and shoot regeneration in Ipomoea obscura (L.): potent Indian medicinal plant. Indian J Sci Technol 2 8:24–26
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15 3:473–497
Nair AG, Vidya P, Sreekumar J, Mohan C (2017) Effect of seed pre-sowing treatment on germination of sweet potato. Int J Appl Pure Sci Agric 3:69–75
Naïtchédé L, Nyende HS, Runo AB, Borlay S AJ (2023) Plant regeneration from embryogenic callus-derived from immature leaves of Momordica Charantia L. Heliyon 9 11:22122
Otani M, Shimada T, Kimura T, SAITO A (1998) Transgenic plant production from embryogenic callus of sweet potato (Ipomoea Batatas (L.) Lam.) using Agrobacterium tumefaciens. Plant Biotechnol 15(1):11–16
Rathnan RK, Anto SM, Emilia B, Katrin J, Ambili M (2013) In vitro callus induction from leaves of Ipomea turpethum and study of antituberculosis activity of leaves and callus extract. WJPPS 2 6:5769–5777
Sanikhani M, Frello S, Serek M (2006) TDZ induces shoot regeneration in various Kalanchoe Blossfeldiana Poelln. Cultivars in the absence of auxin. Plant Cell Tissue Organ Cult 85:75–82
Sefasi A, Kreuze J, Ghislain M, Manrique S, Kiggundu A, Ssemakula G, Mukasa SB (2012) Induction of somatic embryogenesis in recalcitrant sweet potato (Ipomoea Batatas L.) cultivars. Afr J Biotechnol 11 94:16055–16064
Sen MK, Nasrin S, Rahman S, Jamal AHM (2014) In vitro callus induction and plantlet regeneration of Achyranthes aspera L. a high value medicinal plant. Asian Pac J Trop Biomed 4 1:40–46
Torres-Reano G, Alarcon-Bravo L, Rojas-Idrogo C (2017) Seed germination and seedling characteristic of Ipomoea and merremia (Convolvulaceae) in Lambayeque (Peru). Pakistan J Biol Sciences: PJBS 20 10:507–515
We Q, Cao J, Qian W, Xu M, Li Z, Ding Y (2015) Establishment of an efficient micropropagation and callus regeneration system from the axillary buds of Bambusa ventricosa. Plant Cell Tissue Organ Cult 122:1–8
Webster TM, Coble HD (1997) Changes in the weed species composition of the Southern united States: 1974 to 1995. Weed Technol 11 2:308–317
Zia-Ul-Haq M, Riaz M, Feo VD (2012) Ipomoea Hederacea Jacq.: a medicinal herb with promising health benefits. Molecules 17 11:13132–13145
Mbinda W, Anami S, Ombori O, Dixelius C, Oduor R (2016) Efficient plant regeneration of selected Kenyan sweetpotato (Ipomoea batatas (L.) Lam.) cultivars through somatic embryogenesis. J Tissue Eng 7 2:1000176
Author Information
Institute of Biodiversity, School of Ecology and Environmental Sciences, Yunnan University, Kunming, China