In vivo study of efficacy of synthesized silver nanoparticle for quercetin from Bacillus Subtilis against diabetic mice model

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-022-00465-5
First Page: 93
Last Page: 105
Views: 1491

Keywords: Quercetin, Bacillus subtilis , Nanoparticle, Wound healing, Diabetic mice


Quercetin has significant therapeutic applications such as anticancer, anti-fibrosis, and anti-inflammatory agent. It is mainly found in different parts of plant, but, major challenge is the involvement of costly downstream processing techniques for its biosepration. Therefore, in this study, microbial fermentative approach has been employed to achieve its large production at very low cost. Bacillus subtilis MTCC1305 was selected as potent strain for producing intracellular quercetin with production yield of 108.08 mg/ml. The production was enhanced up to 178.58 mg/ml after optimization of media components and fermentation conditions. Development of silver nanoparticle for this purified extract of quercetin has overcame their limitation of poor bioavailability within body fluid. Scanning electron microscopy (SEM) had confirmed the uniform size of its silver nanoparticle as 260 nm. The encapsulation efficiency was obtained as 58% for this silver impregnated purified quercetin extract. The synthesis of nanoparticle was confirmed by Zeta potential value as − 39.5 kV with single peak without any noise property. In diabetic mice model, the nanoparticle treated mice showed significant wound healing during 14th day of treatment. In these treated diabetic mice model, the reepithelization was observed with appearance of different layers of skin similar to that of the control mice. These mice showed improved antioxidant properties with increased level of superoxide dismutase (SOD, 3.6%), catalase (CAT-7.5%), glutathione peroxidase (GPx-4.5%), reduced form of glutathione (GSH-2%) and lipid peroxidase (LPO-10%). These factors showed their effective role for delayed wound healing in diabetic mice.

                Bacillus subtilis
              , Nanoparticle, Wound healing, Diabetic mice

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Author Information

Hooda Himani
Department of Bioscience & Biotechnology, Banasthali Vidyapith, Rajasthan, India

Bajpai Surabhi
Department of Bioscience & Biotechnology, Banasthali Vidyapith, Rajasthan, India

Singh Priyanka
Institute of Allied Medical Science and Technology, NIET, NIMS University, Rajasthan, India