Induction of morphological mutations and mutant phenotyping in black gram [Vigna mungo (L.) Hepper] using gamma rays and EMS

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-019-00057-w
First Page: 464
Last Page: 472
Views: 1791

Keywords: Urdbean, Morphological mutations, Phenotyping, Gamma rays, EMS


Global demand for grain legumes including black gram had increased tremendously in the recent past due to their high nutritional value especially in countries with soaring population growth. This necessitates the induction of genetic variation for continued supply of better yielding and improved varieties to the producers keeping in view the rapidly changing agroclimatic condition. In this study, the induced mutant populations of widely recommended T-9 and Pant U-30 varieties of urdbean were generated using single and combination treatments of gamma rays and ethyl methane sulfonate (EMS). Investigation on induced phenotypic variations in individual plants of M2 population of different treatments resulted in identification and isolation of sixteen morphological mutant types affecting plant height, growth habit, leaf morphology, growth period, pod and seed. Frequency of morphological mutants was the highest in combined treatments of gamma rays and EMS followed by individual treatments of EMS and gamma rays. The spectrum of such mutant types was relatively wide in var. Pant U-30 as compared to the var. T-9. Mutants with altered plant height and pod numbers were of maximum occurrence in both the varieties. The mutants with increased pod number and size were found to be significantly correlated with the improved plant yield, thus selected directly for quantitative investigation in subsequent generations.

Urdbean, Morphological mutations, Phenotyping, Gamma rays, EMS

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The authors are thankful to the University Grants Commission (UGC) New Delhi, India, for providing financial assistance and Aligarh Muslim Uiversity, Aligarh, India for lending infrastructural facilities.

Author Information

Goyal Sonu
Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

Wani Mohammad Rafiq
Department of Botany, Abdul Ahad Azad Memorial Degree College, Cluster University, Srinagar, India

Laskar Rafiul Amin
Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India
Raina Aamir
Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

Amin Ruhul
Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

Khan Samiullah
Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India