Isolation and characterization of Bacillus subtilis RZS-01 isolate from agricultural soil in Bangladesh with potent antimicrobial activities

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-024-00882-8
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Keywords: n Bacillus subtilis sp. RZS-01, 16 s rRNA analysis, Phylogenetic analysis, Antimicrobial activity


Abstract


Plant diseases caused by pathogenic microorganisms, including bacteria, fungi, and other invaders, pose a severe threat to agricultural productivity by impeding plant growth and reducing crop yield. To combat these detrimental effects, harnessing the bioactive properties of indigenous microbial species can play a crucial role in defending plants against pathogenic attacks. In this study, we isolated three Bacillus subtilis strains from the rhizospheric soil of agricultural fields in BARI, Bangladesh. Initial in vitro assessments on culture plates revealed a promising Gram-positive B. subtilis isolate, designated as RZS-01, which displayed potent antibacterial and antifungal effects. Through an integrated approach encompassing biochemical, morphological, and molecular analyses, including 16 s rRNA sequencing and phylogenetic relationships, we confirmed RZS-01 as a member of the Bacillus genus. Notably, this isolate exhibited significant antimicrobial activity against 80% of tested microorganisms, including several notorious plant pathogens such as R. solani, B. cinerea, S. sclerotiorum, Pseudomonas spp., and Xanthomonas spp., as demonstrated in vitro. In the antibiotic susceptibility assay, RZS-01 showed varied sensitivity, with potent antibacterial activity against levofloxacin, nalidixic acid, erythromycin, and ciprofloxacin, resistance to norfloxacin and amoxicillin, and intermediate sensitivity to other antibiotics. These findings pave the way for the development of robust therapeutics aimed at preventing microbial infections in plants, thus enhancing agricultural sustainability and productivity.


n                     Bacillus subtilis sp. RZS-01, 16 s rRNA analysis, Phylogenetic analysis, Antimicrobial activity


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Acknowledgements


The authors express their sincere appreciation for providing all facilities and guidance to the Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka-1205, Bangladesh.


Author Information


Sultana Razia
Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh

Islam Md. Samiul
Graduate School of Agriculture, Hokkaido University, Sapporo, Japan


Hossain Md. Saddam
Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh


Hassan Md. Nazmul
Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science & Technology University, Dinajpur-5200, Bangladesh


Hasan Md. Rakibul
Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh

Shaikh Md. Aftab Ali
Institute of Technology Transfer and Innovation (ITTI), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh