Keywords:
Fungicides, AUDPC, Management, PDI
Blast of Rice (Oryza Sativa L.) caused by Magnaporthe oryzae is a devastating disease that restrain significant yield under field conditions. The efficacy of modern combination fungicides against blast disease of rice was evaluated under in vitro and in UBN (Uniform Blast Nursery) from Telangana at ICAR-IIRR (Indian Institute of Rice Research), Rajendranagar, Hyderabad during Rabi-2022, on the var. HR-12. All fungicides exhibited the considerable levels of inhibition during the study, however, Tricyclazole 70% WP @ 600 ppm was found to be the best concentration with complete inhibition after 14 DAI (Days after inoculation) compared with control. Triazole chemical group in combination with strobilurin group molecules such as Hexaconazole 5% EC, Difenconazole 25% EC, Tebuconazole 250 EC and Azoxystrobin 11% + Tebuconazole 18.3% (w/w) SC) were effective at 500 ppm. Various strobilurin group of molecules in combination with Triazole molecules (Azoxystrobin 18.2% + Difenconazole 11.4% SC; Metiram 55% + Pyraclostrobin (5%) WG; Tebuconazole 50% + Trifloxystrobin 25% w/w (75 WG) were tested for their efficacy under UBN conditions at a constant dosage of 1.0 ml/1 and effectively reduced the blast disease PDI to 26.66%, 20.63% and 14.6%, respectively compared to control PDI of 54.46%. with AUDPC and r 324.8 units −1 and an average of 0.04 units −1 day when compared with control.
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Abed-Ashtiani F, Kadir JB, Selamat AB, Hanif AHBM, Nasehi A (2012) Effect of foliar and root application of silicon against rice blast fungus in MR219 rice variety. Plant Pathol J 28(2):164–171
Amoghavarsha C, Pramesh D, Naik GR, Naik MK, Yadav MK, Ngangkham U, Chidanandappa E, Raghunandana A, Sharanabasav H, Manjunatha SE (2022) Morpho-molecular diversity and avirulence genes distribution among the diverse isolates of Magnaporthe oryzae from Southern India. J Applied Microbiology 132(2):1275–1290
Ashkani S, Yusop MR, Shabanimofrad M, Harun AR, Sahebi M, Latif MA (2015) Genetic analysis of resistance to rice blast. A study on the inheritance of resistance to the blast disease pathogen in an F3 population of rice. J Phytopathol 163:300–309
Avila-Adame C, Köller W (2003) Characterization of spontaneous mutants of Magnaporthe grisea expressing stable resistance to the Qo-inhibiting fungicide azoxystrobin. Curr Genet 42:332–338
Balba H (2007) Review of strobilurin fungicide chemicals. J Environ Sci Health B 42:441–451
Bharanideepan A, Praveen NM, Manasa R, Jeyaprakash P, Muthamilan M, Ramanathan A (2018) Phytopathometry of rice leaf blast for the evaluation of slow blasting resistance genotypes. J Mycology Plant Pathology 48(2):37–48
Chuma I, Isobe C, Hotta Y, Ibaragi K, Futamata N, Kusaba M, Yoshida K, Terauchi R, Fujita Y, Nakayashiki H (2011) Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species. PLoS Pathog 7:e1002147
Dean R, Van Kan JA, Pretorius ZA, Hammond-Kosack KE, Di Pietro A, Spanu PD, Foster GD (2012) The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 13(4):414–430
Ganesh NR, Gangadhara NB, Basavaraja NT, Krishna NR (2012) Fungicidal management of leaf blast disease in rice. J Bio-Sci Biotechnol 1:18–21
Gomez KA, Gomez AA (1984) Statistical procedures for Agricultural Research. John Wiley & Sons
Gonçalves FJ, Corsi de Filippi MC, Silva Lobo VL, Araújo LG, Silva GB, Coelho ASG, Prabhu AS (2016) Polymorphism detection by microsatellite markers in a Magnaporthe oryzae population from different geographical areas of Brazil. J Phytopathol 164(9):620–630
Goswami SK, Tind TS (2018) Efficacy of fungicides against grain discoloration of rice under natural conditions. Indian Phytopathol 71:453–455
Hosseyni-Moghaddam M, Soltani J (2013) An investigation on the effects of photoperiod, aging and culture media on vegetative growth and sporulation of rice blast pathogen Pyricularia oryzae. Progress in Biological Sciences 3(2):135–143
IRRI (2014) Standard Evaluation System for Rice (SES), Los Banos. Philippines: International Rice Research Institute (IRRI) 4: 15–16.
Jagadeesh D, Kumar MK, Amruthavalli C, Devaki NS (2020) Genetic diversity of Magnaporthe oryzae, the blast pathogen of rice in different districts of Karnataka, India determined by simple sequence repeat (SSR) markers. Indian Phytopathology 73(4):713–723
Kongcharoen N, Kaewsalong N, Dethoup T (2020) Efficacy of fungicides in controlling rice blast and dirty panicle diseases in Thailand. Sci Rep 10(1):1–7
Kramer W, Schirmer U (2007) Modern crop protection compounds, 3. John Wiley & Sons Ltd
Kumar MKP, Gowda DKS, Moudgal R, Kumar NK, Gowda KTP, Vishwanath K (2013) Impact on fungicides on rice production in India. In: Nita M (ed) Fungicides-showcases of integrated plant disease management from around the world. Intech, USA, Nappanee
Kumar S, Kashyap PL, Mahapatra S, Jasrotia P, Singh GP (2021) New and emerging technologies for detecting Magnaporthe oryzae causing blast disease in crop plants. Crop Prot 143:105473
Magar PB, Acharya B, Pandey B (2015) Use of chemicals for the management of rice blast (Pyricularia grisea) disease at Jyotinagar, Chitwan. Nepal Int J Appl Sci Biotech 3(3):474–478
Mohiddin FA, Bhat NA, Wani SH, Bhat AH, Ahanger MA, Shikari AB, Sabagh AE (2021) Combination of strobilurin and triazole chemicals for the management of blast disease in Mushk Budji-aromatic rice. J Fungi 7(12):1060
Nene YL, Thapliyal PN (1993) Fungicides in plant disease control. International Science Publisher
Pak D, You MP, Lanoiselet V, Barbetti MJ (2017) Azoxystrobin and propiconazole offer significant potential for rice blast (Pyricularia oryzae) management in Australia. Eur J Plant Pathol 148:247–259
Panse VG, Sukhathme PV (1985) Statistical methods for agricultural workers, 4th edn. ICAR, New Delhi, p 347
Pramesh D, Nataraj K, Guruprasad GS, Mahantashivayogayya K, Reddy BGM (2016) Evaluation of a new strobilurin group of fungicide for the management of blast disease of paddy. Am J Exp Agric 13:1–6
Pramesh D, Alase S, Muniraju KM, Kumara MK (2017) A combination fungicide for the management of sheath blight, sheath rot and stem rot diseases of paddy. Int J Curr Microbiol App Sci 6:3500–3509
Raj R, Pannu PPS (2017) Management of rice blast with different fungicides and potassium silicate under in vitro and in vivo conditions. J Plant Pathol 99:707–712
Sakulkoo W, Oses-Ruiz M, Oliveira Garcia E, Soanes DM, Littlejohn GR, Hacker C, Correia A, Valent B, Talbot NJ (2018) A single fungal MAP kinase controls plant cell-to-cell invasion by the rice blast fungus. Science 359:1399–1403
Sethy PK, Barpanda NK, Rath AK, Behera SK (2020) Image processing techniques for diagnosing rice plant disease: a survey. Procedia Computer Science 167:516–530
Skamnioti P, Gurr SJ (2009) Against the grain: safeguarding rice from rice blast disease. Trends Biotechnol 27(3):141–150
Tenni D, Sinetti A, Waldner M, Torriani SF, Romani M (2021) First report of QoI resistance in Italian population of Pyricularia oryzae. J Plant Dis Prot 128(6):1705–1709
Tuite J (1969) Plant pathological methods. Fungi and bacteria. In: Plant pathological methods. Fungi and bacteria
Van der Plank JE (1963) Plant diseases. Elsevier Science
Wheeler BEJ (1969) An introduction to plant diseases. John Wiley and Sons Ltd, London., p 301
Wheeler MH (1982) Melanin biosynthesis in Verticillium daliae: Dehydratation and reduction reactions in cell-free homogenates. Exp Mycol 6:171–179
Wilcoxson RD, Skovmand B, Atif AA (1975) Evaluation of wheat cultivars for the ability to retard development of stem rust. Annal Appl Biol 80:275–287
Wilson RA, Talbot NJ (2009) Under pressure: investigating the biology of plant infection by Magnaporthe oryzae. Nat Rev Microbiol 7(3):185–195
The authors would like to thank the Department of Plant Pathology, Department of Biotechnology, Professor Jayashankar Telangana State Agricultural University, Hyderabad and ICAR-IIRR for rendering necessary facilities providing support during the time of investigation