Media optimization using Box Behnken design for enhanced production of biomass, beta-carotene and lipid from Dunaliella salina

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-019-00079-4
First Page: 31
Last Page: 39
Views: 1815

Keywords: Beta-carotene, Lipids, Box Behnken design, Response surface methodology


Dunaliella salina a halotolerant microalga is well known for a high fatty acid and beta-carotene content, which makes it a potent source at a commercial level. The current study focuses on optimizing commercially known media using Box Behnken design to attain higher yields of biomass, beta-carotene, and lipids simultaneously. The optimal medium conditions as per response surface methodology were glucose, potassium nitrate, sodium chloride at a concentration of 13.23 g/L, 3.145 g/L and 35.6 g/L, respectively while maintaining the concentration of other nutrients unchanged. Maximum yield of biomass, beta-carotene and lipid productivity attained experimentally using the optimized media was 1.24 g/L, 6.07 mg/g and 20.7 mg/L/day, respectively than their original values i.e., 0.571 g/L of biomass, 4.18 mg/g of beta-carotene and 13.2 mg/L/day of lipid content. Biomass yield was increased by 2.17 folds, beta-carotene and lipid were increased by 1.45 folds and 1.56 folds, respectively.

Beta-carotene, Lipids, Box Behnken design, Response surface methodology

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This study was supported by Department of Biotechnology, Delhi Technological University, New Delhi.

Author Information

Ahuja Shruti
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India

Roy Arpita
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India

Kumar Lakhan
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India

Bharadvaja Navneeta
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India