Micropropagation of threatened medicinal plant Andrographis lineata var. lawii

,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00585-6
First Page: 202
Last Page: 210
Views: 1463


Keywords: Cotyledon cultures, In vitro propagation, Nodal cultures, Shoot tip cultures


Abstract


Andrographis lineata var. lawii is a threatened and endemic species that yield several therapeutic flavonoids. In the present study, a simple and efficient in vitro plant regeneration protocol was developed using shoot tip, nodal and cotyledonary explants. Shoot tip and nodal explants were involved in direct regeneration of multiple shoots on Murashige and Skoog medium supplemented with benzyl amino purine (BAP). An average of 24.83 and 21.41 shoots were regenerated from shoot tip and nodal explants on MS medium containing 7.5 µM and 10 µM BAP, respectively. Whereas, cotyledonary leaf explants developed callus-mediated multiple shoot buds on thidiazuron (TDZ) supplemented medium from petiolar regions, however, shoot buds did not propagated further. Upon sub-culturing of callus-containing shoot buds on MS medium containing BAP, proliferation and elongation were observed and optimal results were realized on an MS medium supplemented with 2 µM BAP. Histological analysis was carried out to reveal the early events of shoot regeneration. Rooting of shoots was achieved on 1/4 MS medium containing 10 µM indole butyric acid (IBA). The in vitro regeneration methodology is useful for the mass production of plants and germplasm preservation.


Cotyledon cultures, In vitro propagation, Nodal cultures, Shoot tip cultures


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Abu-Ghefreh AA, Canatan H, Ezeamuzie CI (2009) In vitro and in vivo anti-inflammatory effects of andrographolide. Int Immunopharmacol 9:313–318


Akowuah GA, Zhari I, Mariam A (2008) Analysis of urinary andrographolides and antioxidant status after oral administration of Andrographis paniculata leaf extract in rats. Food Chem Toxicol 46:3616–3620


Alagesaboopathi C (2011) Phytochemical analysis and antimicrobial evaluation of Andrographis lineata nees leaves and stem extracts. Afr J Pham Pharmacol 5:1190–1195


Alagesaboopathi C (2012) Ethnobotany of Andrographis lineata Wallich ex Nees - An endemic medicinal plant of India. Int J Recent Sci Res 3(2):71–74


Aruna V, Pullaiah T (2016) In vitro shoot multiplication of medicinally important plant Andrographis lineata nees via nodal explants. EJMP 17(3):1–7


Bouddha S, Bharti S, Rai E, Ansari SA (2020) A protocol for in vitro somatic embryogenesis from leaf explants of Andrographis paniculata Wall. Ex nees. Ind J Bot Soc 99:56–65


Dalawai D, Aware C, Jadhav JP, Murthy HN (2019) RP-HPLC analysis of diterpene lactones in leaves and stem of different species of Andrographis. Nat Prod Res 35(13):2239–2242


Dandin VS, HN Murthy (2012a) Regeneration of Andrographis paniculata nees: analysis of genetic fidelity and andrographolide content in micropropagated plants. Afr J Biotechnol 11:12464–12471


Dandin VS, HN Murthy (2012b) Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants. Acta Physiol Plant 24:1381–1386


Dandin VS, Naik PM, Murthy HN, Park SY, Lee EJ, Paek KY (2014) Rapid plant regeneration and analysis of genetic fidelity and scopoletin contents of micropropagated plants of Spilanthes oleracea L. J Hortic Sci Biotechnol 89(1):79–85


Deepa VS, Rajaram K, Kumar PS (2013) In vitro and in vivo antidiabetic effect of Andrographis lineata Wall. Ex. Nees and Andrographis serphyllifolia Wt. Ic leaf extracts. Afr J Pham and Pharmacol 7:2112–2121


Deepa VS, Rajaram K, Kumar MA, Das S, Kumar PS (2011) High frequency regeneration and shoot multiplication in Andrographis lineata Wall. Ex. Nees: an endemic medicinal plant of South India. J Med Plants Res 5:5044–5049


Gamble JS (1956) Flora of the Presidency of Madras. Botanical Survey of India, Calcutta, Vol. 2, p. 1052


Ghosh B, Sen S (1994) Micropropagation of Asparagus cooperi as affected by growth regulators. Biol Plant 36(4):527–534


Huang H, Wei Y, Zhai Y, Ouyang K, Chen X, Bai L (2020) High frequency regeneration of plants via callus-mediated organogenesis from cotyledon and hypocotyl cultures in a multipurpose tropical tree (Neolamarkia cadamba). Sci Rep 10(1):1–10


Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinins for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105–119


Ignacimuthu S, Ayyanar M, Sharasivaraman K (2006) Ethnobotanical investigations among the tribes in Madurai district of Tamilnadu (India). J Ethno Enthanomed 2:1–20


Johansen DA (1940) Plant microtechnique. MCGraw-Hill Co., New York


Kadapatti SS, Murthy HN (2021a) Rapid plant regeneration, analysis of genetic fidelity and neoandrographolide content of micropropagated plants of Andrographis alata (Vahl) nees. J Genet Eng Biotechnol 19(1):20


Kadapatti SS, Murthy HN (2021b) In vitro micropropagation of Andrographis macrobotrys doi.org/10.1080/10496475.2021.1964012


Kadapatti SS, Murthy HN (2022) Micropropagation of Andrographis producta through axillary and adventitious shoot regeneration. J Genet Eng Biotechnol 20:152


Kantayos V, Bae CH (2019) Optimized shoot induction and histological study of in vitro cultured korean soybean cultivars. Korean J Plant Res 32(3):237–243


Kishore HP, Reddy MV, Reddy MK, Gunasekar D, Caux C, Bodo B (2003) Flavonoids from Andrographis lineata. Phytochemistry 63:457–461


Kshirsagar RD, Singh NP (2001) Some less known ethnomedicinal uses from Mysore and Coorg districts, Karnataka state India. J Ethnopharmacol 75:231–238


Kumar RA, Sridevi K, Kumar NV, Nanduri S, Rajagopal S (2004) Anticancer and immunostimulatory compounds from Andrographis paniculata. J Ethnopharmacol 92:291–295


Lee YC, Lin HH, Hsu CH, Wang CJ, Chiang TA, Chen JH (2010) Inhibitory effects of andrographolide on migration and invasion in human non-small cell lung cancer A549 cells via down-regulation of PI3K/Akt signaling pathway. Eur Pharmacol 632:23–32


Mohammed A, Chiruvella KK, Ghanta RG (2016) In vitro plant regeneration, flowering and fruiting from nodal explants of Andrographis lineata nees (Acanthaceae). J Crop Sci Biotech 19:195–202


Mohammed A, Chiruvella KK, Rao YK, Geethangili M, Raghavan SC, Ghanta RG (2015) In vitro production of echioidinin, 7-o-methywogonin from callus cultures of Andrographis lineata and their cytotoxicity on cancer cells. PLoS ONE 10:e0141154


Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497


Neeraja C, Krishna PH, Reddy CS, Giri CC, Rao KV, Reddy VD (2015) Distribution of Andrographis species in different districts of Andhra Pradesh. Proc Natl Acad Sci India Sect B Biol Sci 85:601–606


Pendli S, Rohela GK, Jogam P, Bylla P, Korra Thammidala C (2019) High frequency in vitro plantlet regeneration in Solanum trilobatum L., an important ethno-medicinal plant and confirmation of genetic fidelity of R1 plantlets by using ISSR and RAPD markers. Vegetos 32:508–520


Purkayastha J, Sugla T, Paul A, Solleti S, Sahoo L (2008) Rapid in vitro multiplication and plant regeneration from nodal explants of Andrographis paniculata: a valuable medicinal plant. In Vitro Cell Dev Biol Plant 44(5):442–447


Shailaja A, Bindu BBV, Srinath M, Giri CC (2020) Innovative technique for rapid in vitro multiplication of rootless shoots in Andrographis paniculata (burm. F) nees: a plant with immense pharmaceutical value. Ann Phytomed 9(1):98–106


Sheeja K, Shihab PK, Kuttan G (2006) Antioxidant and anti-inflammatory activities of the plant Andrographis paniculata nees. Immunopharmacol Immunotoxicol 28:129–140


Shen T, Yang WS, Yi YS, Sung GH, Rhee MH, Poo H, Kim KW, Kim JH, Cho JY (2013) Ap-1/IRF-3 targeted anti-inflammatory activity of andrographolide isolated from Andrographis paniculata. Evid Based Complement Altern Med 210736


Singh M, Yadav C, Bano F (2018) Plant regeneration from alginate encapsulated nodes of Andrographis paniculata (burm. F.) wallich ex nees. A potential medicinal herb. Vegetos 31:28–33


Singha PK, Roy S, Dey S (2003) Antimicrobial activity of Andrographis paniculata. Fitoterapia 74:692–694


Trivedi NP, Rawal UM, Patel BP (2007) Hepatoprotective effects of andrographolide against hexachlorocyclohexane-induced oxidative injury. Integr Cancer Ther 6:271–280


Xavier TF, Kumar S (2018) Indirect organogenesis of Andrographis echioides (L.) nees. World J Pharm Res 7(5):1655–1664


Savitikadi P, Jogam P, Rohela GK, Ellendula R, Sandhya D, Allini VR, Abbagani S (2020) Direct regeneration and genetic fidelity analysis of regenerated plants of Andrographis echiodes (L.) - An important medicinal plant. Ind Crops Prod 155:112766

 


Acknowledgements


Hosakatte Niranjana Murthy is thankful for the ?Brain Pool (BP)? program Grant No.2022H1D3A2A02056665.


Author Information


Kadapatti Sathish Shekhappa
Department of Botany, Karnatak University, Dharwad, India

Murthy Hosakatte Niranjana
Department of Botany, Karnatak University, Dharwad, India
hnmurthy60@gmail.com