Vegetos
(An International Journal of Plant Research & Biotechnology)
Microscopic characterization and proximate analysis of Belosynapsis vivipara leaves: a rare plant of Western Ghats region
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Research Articles | Published: 15 June, 2025
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Keywords: n Belosynapsis viviparan , GC–MS, Microscopy, Proximate analysis, SEM
Abstract
Belosynapsis vivipara (Dalzell) C.E.C. Fisch. (F: Commelinaceae) is distributed throughout Western Ghats regions in India. The plant is not well known in scientific platforms due to lack of availability in all the places in India. The plant was identified and described earlier but its therapeutic activities and other botanical aspects are still in demand. Traditionally, the leaves were established as a potential part for human applications. With that, the aim of the present study was carried out with the microscopic method and SEM analysis for determination of the anatomical arrangement and histological nature of the leaves and various proximate analyses such as moisture content, ash content, extractive values, crude fiber content, crude fat content, protein content and carbohydrate content were also determined. The fresh and dried leaves showed the presence of moisture content of 18.46% and 6.12%, total ash of 4.04% and 9.43%, alcohol-soluble extractives of 15.11% and 18.32%, water-soluble extractives of 11.08% and 15.34%, crude fiber of 8.08% and 11.61%, crude fat of 4.01% and 2.32%, and crude protein of 11.02% and 4.14%, respectively. GCMS study revealed the presence of 25 important bioactive compounds among them, five main compounds were identified and confirmed with the TLC and HPLC analysis, i.e., stigmasterol, beta-sitosterol, lupeol, gallic acid, and squalene. Finally, the overall result concluded that the microscopic analysis, proximate parameters, and bioactive compounds can serve as tools for the development of standards for proper authentication, quality, and purity of Belosynapsis vivipara leaves.
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Author Information
Department of Pharmacognosy and Phytochemistry, Mallige College of Pharmacy, Bengaluru, India